Experiment: Carbon source L-Ornithine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05990 and PGA1_c06000 are separated by 58 nucleotides PGA1_c06000 and PGA1_c06010 are separated by 40 nucleotides
PGA1_c05990: PGA1_c05990 - formate--tetrahydrofolate ligase Fhs, at 612,539 to 614,215
_c05990
PGA1_c06000: PGA1_c06000 - chorismate mutase-like protein, at 614,274 to 614,579
_c06000
PGA1_c06010: PGA1_c06010 - bifunctional protein FolD, at 614,620 to 615,522
_c06010
Position (kb)
614
615 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 613.293 kb on - strand, within PGA1_c05990 at 613.312 kb on + strand, within PGA1_c05990 at 613.320 kb on - strand, within PGA1_c05990 at 613.449 kb on + strand, within PGA1_c05990 at 613.462 kb on - strand, within PGA1_c05990 at 613.533 kb on - strand, within PGA1_c05990 at 613.533 kb on - strand, within PGA1_c05990 at 613.614 kb on + strand, within PGA1_c05990 at 613.650 kb on - strand, within PGA1_c05990 at 613.658 kb on - strand, within PGA1_c05990 at 613.708 kb on + strand, within PGA1_c05990 at 613.726 kb on - strand, within PGA1_c05990 at 613.737 kb on + strand, within PGA1_c05990 at 613.751 kb on - strand, within PGA1_c05990 at 613.852 kb on + strand, within PGA1_c05990 at 613.852 kb on + strand, within PGA1_c05990 at 613.857 kb on + strand, within PGA1_c05990 at 613.924 kb on + strand, within PGA1_c05990 at 614.185 kb on - strand at 614.205 kb on - strand at 614.307 kb on + strand, within PGA1_c06000 at 614.339 kb on + strand, within PGA1_c06000 at 614.348 kb on + strand, within PGA1_c06000 at 614.417 kb on + strand, within PGA1_c06000 at 614.497 kb on + strand, within PGA1_c06000 at 614.548 kb on + strand, within PGA1_c06000 at 614.586 kb on + strand at 614.586 kb on + strand at 614.586 kb on + strand at 614.586 kb on + strand at 614.586 kb on + strand at 614.586 kb on + strand at 614.594 kb on - strand at 614.594 kb on - strand at 614.618 kb on - strand at 615.491 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source L-Ornithine 5 mM remove 613,293 - PGA1_c05990 0.45 +0.4 613,312 + PGA1_c05990 0.46 -1.2 613,320 - PGA1_c05990 0.47 -0.4 613,449 + PGA1_c05990 0.54 -1.0 613,462 - PGA1_c05990 0.55 -1.2 613,533 - PGA1_c05990 0.59 -1.6 613,533 - PGA1_c05990 0.59 -1.0 613,614 + PGA1_c05990 0.64 -1.2 613,650 - PGA1_c05990 0.66 -0.9 613,658 - PGA1_c05990 0.67 -1.0 613,708 + PGA1_c05990 0.70 -0.1 613,726 - PGA1_c05990 0.71 -1.1 613,737 + PGA1_c05990 0.71 +0.1 613,751 - PGA1_c05990 0.72 -0.9 613,852 + PGA1_c05990 0.78 +0.8 613,852 + PGA1_c05990 0.78 -0.4 613,857 + PGA1_c05990 0.79 -0.7 613,924 + PGA1_c05990 0.83 +0.0 614,185 - -1.9 614,205 - +0.6 614,307 + PGA1_c06000 0.11 -1.8 614,339 + PGA1_c06000 0.21 -1.0 614,348 + PGA1_c06000 0.24 -1.4 614,417 + PGA1_c06000 0.47 +0.6 614,497 + PGA1_c06000 0.73 -0.2 614,548 + PGA1_c06000 0.90 -0.7 614,586 + -0.6 614,586 + +0.4 614,586 + -1.6 614,586 + +0.8 614,586 + -0.0 614,586 + +0.3 614,594 - -1.4 614,594 - +1.8 614,618 - +0.5 615,491 + -0.3
Or see this region's nucleotide sequence