Experiment: Carbon source L-Ornithine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c11750 and PGA1_c11760 are separated by 135 nucleotides PGA1_c11760 and PGA1_c11770 are separated by 69 nucleotides PGA1_c11770 and PGA1_c11780 are separated by 109 nucleotides
PGA1_c11750: PGA1_c11750 - L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88); Proline dehydrogenase (EC 1.5.5.2) (from data) , at 1,215,988 to 1,219,464
_c11750
PGA1_c11760: PGA1_c11760 - putative proline dehydrogenase transcriptional activator, at 1,219,600 to 1,220,073
_c11760
PGA1_c11770: PGA1_c11770 - tetratricopeptide repeat-containing protein, at 1,220,143 to 1,220,775
_c11770
PGA1_c11780: PGA1_c11780 - tRNA-Leu, at 1,220,885 to 1,220,970
_c11780
Position (kb)
1219
1220
1221 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1218.669 kb on + strand, within PGA1_c11750 at 1218.736 kb on + strand, within PGA1_c11750 at 1218.736 kb on + strand, within PGA1_c11750 at 1218.945 kb on - strand, within PGA1_c11750 at 1218.945 kb on - strand, within PGA1_c11750 at 1218.985 kb on + strand, within PGA1_c11750 at 1218.985 kb on + strand, within PGA1_c11750 at 1218.990 kb on + strand, within PGA1_c11750 at 1219.055 kb on + strand, within PGA1_c11750 at 1219.055 kb on + strand, within PGA1_c11750 at 1219.063 kb on - strand, within PGA1_c11750 at 1219.073 kb on - strand, within PGA1_c11750 at 1219.076 kb on + strand, within PGA1_c11750 at 1219.175 kb on + strand at 1219.180 kb on + strand at 1219.180 kb on + strand at 1219.180 kb on + strand at 1219.239 kb on + strand at 1219.395 kb on + strand at 1219.402 kb on + strand at 1219.651 kb on + strand, within PGA1_c11760 at 1219.651 kb on + strand, within PGA1_c11760 at 1219.665 kb on - strand, within PGA1_c11760 at 1219.687 kb on - strand, within PGA1_c11760 at 1219.748 kb on - strand, within PGA1_c11760 at 1219.803 kb on + strand, within PGA1_c11760 at 1219.897 kb on + strand, within PGA1_c11760 at 1219.897 kb on + strand, within PGA1_c11760 at 1219.989 kb on + strand, within PGA1_c11760 at 1220.017 kb on + strand, within PGA1_c11760 at 1220.025 kb on - strand, within PGA1_c11760 at 1220.060 kb on - strand at 1220.070 kb on - strand at 1220.133 kb on + strand at 1220.133 kb on + strand at 1220.151 kb on + strand at 1220.151 kb on + strand at 1220.151 kb on + strand at 1220.151 kb on + strand at 1220.159 kb on - strand at 1220.159 kb on - strand at 1220.210 kb on - strand, within PGA1_c11770 at 1220.224 kb on - strand, within PGA1_c11770 at 1220.230 kb on + strand, within PGA1_c11770 at 1220.290 kb on - strand, within PGA1_c11770 at 1220.290 kb on - strand, within PGA1_c11770 at 1220.305 kb on + strand, within PGA1_c11770 at 1220.466 kb on + strand, within PGA1_c11770 at 1220.548 kb on + strand, within PGA1_c11770 at 1220.556 kb on - strand, within PGA1_c11770 at 1220.556 kb on - strand, within PGA1_c11770 at 1220.556 kb on - strand, within PGA1_c11770 at 1220.556 kb on - strand, within PGA1_c11770 at 1220.556 kb on - strand, within PGA1_c11770 at 1220.570 kb on + strand, within PGA1_c11770 at 1220.580 kb on - strand, within PGA1_c11770 at 1220.599 kb on + strand, within PGA1_c11770 at 1220.599 kb on + strand, within PGA1_c11770 at 1220.665 kb on + strand, within PGA1_c11770 at 1220.711 kb on - strand, within PGA1_c11770 at 1220.769 kb on + strand at 1220.777 kb on + strand at 1220.870 kb on + strand at 1220.870 kb on + strand at 1221.030 kb on + strand at 1221.070 kb on + strand at 1221.073 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source L-Ornithine 5 mM remove 1,218,669 + PGA1_c11750 0.77 -2.9 1,218,736 + PGA1_c11750 0.79 -3.1 1,218,736 + PGA1_c11750 0.79 -0.8 1,218,945 - PGA1_c11750 0.85 -2.6 1,218,945 - PGA1_c11750 0.85 -3.1 1,218,985 + PGA1_c11750 0.86 -2.0 1,218,985 + PGA1_c11750 0.86 -1.0 1,218,990 + PGA1_c11750 0.86 -0.7 1,219,055 + PGA1_c11750 0.88 -0.5 1,219,055 + PGA1_c11750 0.88 -1.4 1,219,063 - PGA1_c11750 0.88 -1.3 1,219,073 - PGA1_c11750 0.89 -1.5 1,219,076 + PGA1_c11750 0.89 -0.7 1,219,175 + -1.9 1,219,180 + -2.3 1,219,180 + -0.2 1,219,180 + -3.2 1,219,239 + +1.6 1,219,395 + -2.0 1,219,402 + -2.6 1,219,651 + PGA1_c11760 0.11 -2.0 1,219,651 + PGA1_c11760 0.11 -1.9 1,219,665 - PGA1_c11760 0.14 -1.6 1,219,687 - PGA1_c11760 0.18 -3.0 1,219,748 - PGA1_c11760 0.31 -0.7 1,219,803 + PGA1_c11760 0.43 -0.9 1,219,897 + PGA1_c11760 0.63 -1.8 1,219,897 + PGA1_c11760 0.63 -1.8 1,219,989 + PGA1_c11760 0.82 -1.1 1,220,017 + PGA1_c11760 0.88 -2.4 1,220,025 - PGA1_c11760 0.90 -1.4 1,220,060 - -2.6 1,220,070 - -1.0 1,220,133 + -1.4 1,220,133 + -0.8 1,220,151 + -0.9 1,220,151 + +0.9 1,220,151 + -0.8 1,220,151 + -1.0 1,220,159 - +0.6 1,220,159 - -0.6 1,220,210 - PGA1_c11770 0.11 +0.1 1,220,224 - PGA1_c11770 0.13 -2.0 1,220,230 + PGA1_c11770 0.14 +0.6 1,220,290 - PGA1_c11770 0.23 -0.1 1,220,290 - PGA1_c11770 0.23 -0.1 1,220,305 + PGA1_c11770 0.26 +0.3 1,220,466 + PGA1_c11770 0.51 -0.4 1,220,548 + PGA1_c11770 0.64 -1.5 1,220,556 - PGA1_c11770 0.65 +0.6 1,220,556 - PGA1_c11770 0.65 +0.4 1,220,556 - PGA1_c11770 0.65 -0.8 1,220,556 - PGA1_c11770 0.65 +0.5 1,220,556 - PGA1_c11770 0.65 -1.4 1,220,570 + PGA1_c11770 0.67 +0.6 1,220,580 - PGA1_c11770 0.69 -2.1 1,220,599 + PGA1_c11770 0.72 -0.7 1,220,599 + PGA1_c11770 0.72 -0.2 1,220,665 + PGA1_c11770 0.82 -0.7 1,220,711 - PGA1_c11770 0.90 +0.8 1,220,769 + +1.8 1,220,777 + +0.6 1,220,870 + -0.0 1,220,870 + +0.3 1,221,030 + -0.2 1,221,070 + +0.9 1,221,073 - -0.3
Or see this region's nucleotide sequence