Strain Fitness in Ralstonia sp. UNC404CL21Col around ABZR87_RS00150

Experiment: Ra3_1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABZR87_RS00135 and ABZR87_RS00140 are separated by 55 nucleotidesABZR87_RS00140 and ABZR87_RS00145 overlap by 4 nucleotidesABZR87_RS00145 and ABZR87_RS00150 are separated by 22 nucleotidesABZR87_RS00150 and ABZR87_RS00155 are separated by 114 nucleotides ABZR87_RS00135: ABZR87_RS00135 - dihydroxy-acid dehydratase, at 26,837 to 28,510 _RS00135 ABZR87_RS00140: ABZR87_RS00140 - DUF2486 family protein, at 28,566 to 28,991 _RS00140 ABZR87_RS00145: ABZR87_RS00145 - DNA polymerase III subunit chi, at 28,988 to 29,416 _RS00145 ABZR87_RS00150: ABZR87_RS00150 - leucyl aminopeptidase, at 29,439 to 30,947 _RS00150 ABZR87_RS00155: ABZR87_RS00155 - LPS export ABC transporter permease LptF, at 31,062 to 32,180 _RS00155 Position (kb) 29 30 31Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 28.439 kb on + strandat 28.439 kb on + strandat 28.439 kb on + strandat 28.440 kb on - strandat 28.441 kb on + strandat 28.441 kb on + strandat 28.442 kb on - strandat 28.442 kb on - strandat 28.442 kb on - strandat 28.442 kb on - strandat 28.899 kb on + strand, within ABZR87_RS00140at 28.899 kb on + strand, within ABZR87_RS00140at 28.899 kb on + strand, within ABZR87_RS00140at 28.900 kb on - strand, within ABZR87_RS00140at 28.900 kb on - strand, within ABZR87_RS00140at 28.975 kb on + strandat 29.371 kb on - strand, within ABZR87_RS00145at 29.416 kb on + strandat 29.416 kb on + strandat 29.416 kb on + strandat 29.416 kb on + strandat 29.416 kb on + strandat 29.416 kb on + strandat 29.417 kb on - strandat 29.417 kb on - strandat 29.417 kb on - strandat 29.417 kb on - strandat 29.551 kb on + strandat 29.656 kb on + strand, within ABZR87_RS00150at 29.657 kb on - strand, within ABZR87_RS00150at 29.657 kb on - strand, within ABZR87_RS00150at 29.657 kb on - strand, within ABZR87_RS00150at 29.746 kb on + strand, within ABZR87_RS00150at 29.746 kb on + strand, within ABZR87_RS00150at 29.746 kb on + strand, within ABZR87_RS00150at 29.747 kb on - strand, within ABZR87_RS00150at 29.747 kb on - strand, within ABZR87_RS00150at 29.747 kb on - strand, within ABZR87_RS00150at 29.747 kb on - strand, within ABZR87_RS00150at 29.839 kb on + strand, within ABZR87_RS00150at 29.839 kb on + strand, within ABZR87_RS00150at 29.839 kb on + strand, within ABZR87_RS00150at 29.840 kb on - strand, within ABZR87_RS00150at 29.840 kb on - strand, within ABZR87_RS00150at 29.840 kb on - strand, within ABZR87_RS00150at 29.840 kb on - strand, within ABZR87_RS00150at 29.840 kb on - strand, within ABZR87_RS00150at 29.840 kb on - strand, within ABZR87_RS00150at 29.840 kb on - strand, within ABZR87_RS00150at 29.840 kb on - strand, within ABZR87_RS00150at 29.840 kb on - strand, within ABZR87_RS00150at 30.058 kb on + strand, within ABZR87_RS00150at 30.058 kb on + strand, within ABZR87_RS00150at 30.058 kb on + strand, within ABZR87_RS00150at 30.058 kb on + strand, within ABZR87_RS00150at 30.058 kb on + strand, within ABZR87_RS00150at 30.059 kb on - strand, within ABZR87_RS00150at 30.059 kb on - strand, within ABZR87_RS00150at 30.059 kb on - strand, within ABZR87_RS00150at 30.169 kb on + strand, within ABZR87_RS00150at 30.169 kb on + strand, within ABZR87_RS00150at 30.319 kb on + strand, within ABZR87_RS00150at 30.320 kb on - strand, within ABZR87_RS00150at 30.320 kb on - strand, within ABZR87_RS00150at 30.521 kb on - strand, within ABZR87_RS00150at 30.985 kb on + strandat 30.985 kb on + strandat 30.985 kb on + strandat 30.986 kb on - strandat 30.988 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ra3_1
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28,439 + -1.3
28,439 + +0.8
28,439 + +0.1
28,440 - +0.3
28,441 + +1.2
28,441 + +0.8
28,442 - +0.4
28,442 - -0.0
28,442 - -1.1
28,442 - -2.0
28,899 + ABZR87_RS00140 0.78 +3.1
28,899 + ABZR87_RS00140 0.78 +2.1
28,899 + ABZR87_RS00140 0.78 +2.7
28,900 - ABZR87_RS00140 0.78 +4.0
28,900 - ABZR87_RS00140 0.78 +3.3
28,975 + +3.5
29,371 - ABZR87_RS00145 0.89 +0.3
29,416 + +0.8
29,416 + +1.7
29,416 + +0.4
29,416 + +2.9
29,416 + +2.0
29,416 + +2.4
29,417 - -0.9
29,417 - -0.0
29,417 - -0.6
29,417 - -1.5
29,551 + +2.6
29,656 + ABZR87_RS00150 0.14 +1.6
29,657 - ABZR87_RS00150 0.14 +1.5
29,657 - ABZR87_RS00150 0.14 +0.5
29,657 - ABZR87_RS00150 0.14 +1.0
29,746 + ABZR87_RS00150 0.20 +2.6
29,746 + ABZR87_RS00150 0.20 +2.0
29,746 + ABZR87_RS00150 0.20 +1.7
29,747 - ABZR87_RS00150 0.20 +1.2
29,747 - ABZR87_RS00150 0.20 +0.7
29,747 - ABZR87_RS00150 0.20 -0.1
29,747 - ABZR87_RS00150 0.20 +1.2
29,839 + ABZR87_RS00150 0.27 +2.3
29,839 + ABZR87_RS00150 0.27 +2.1
29,839 + ABZR87_RS00150 0.27 +1.7
29,840 - ABZR87_RS00150 0.27 +0.5
29,840 - ABZR87_RS00150 0.27 +0.7
29,840 - ABZR87_RS00150 0.27 +0.0
29,840 - ABZR87_RS00150 0.27 +1.6
29,840 - ABZR87_RS00150 0.27 +1.5
29,840 - ABZR87_RS00150 0.27 +2.3
29,840 - ABZR87_RS00150 0.27 +1.2
29,840 - ABZR87_RS00150 0.27 +0.4
29,840 - ABZR87_RS00150 0.27 +0.4
30,058 + ABZR87_RS00150 0.41 +1.9
30,058 + ABZR87_RS00150 0.41 +3.2
30,058 + ABZR87_RS00150 0.41 +1.5
30,058 + ABZR87_RS00150 0.41 +2.7
30,058 + ABZR87_RS00150 0.41 +2.7
30,059 - ABZR87_RS00150 0.41 +0.3
30,059 - ABZR87_RS00150 0.41 +0.5
30,059 - ABZR87_RS00150 0.41 +0.7
30,169 + ABZR87_RS00150 0.48 +2.0
30,169 + ABZR87_RS00150 0.48 +2.9
30,319 + ABZR87_RS00150 0.58 +2.4
30,320 - ABZR87_RS00150 0.58 +1.9
30,320 - ABZR87_RS00150 0.58 +0.9
30,521 - ABZR87_RS00150 0.72 +0.7
30,985 + +2.6
30,985 + +1.6
30,985 + +2.9
30,986 - -0.2
30,988 + -0.4

Or see this region's nucleotide sequence