Experiment: RCH2_defined_glucose_5mM with Epiandrosterone 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_000690 and ABIE53_000691 are separated by 38 nucleotides ABIE53_000691 and ABIE53_000692 overlap by 11 nucleotides ABIE53_000692 and ABIE53_000693 are separated by 73 nucleotides
ABIE53_000690: ABIE53_000690 - glucosamine--fructose-6-phosphate aminotransferase (isomerizing), at 744,428 to 745,444
_000690
ABIE53_000691: ABIE53_000691 - glucose-specific phosphotransferase system IIA component, at 745,483 to 746,124
_000691
ABIE53_000692: ABIE53_000692 - phosphoenolpyruvate-protein phosphotransferase, at 746,114 to 748,048
_000692
ABIE53_000693: ABIE53_000693 - PTS system N-acetylglucosamine-specific IIC component, at 748,122 to 749,897
_000693
Position (kb) 746
747
748
749 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 745.186 kb on - strand, within ABIE53_000690 at 745.237 kb on + strand, within ABIE53_000690 at 745.358 kb on + strand at 745.358 kb on + strand at 745.358 kb on + strand at 745.358 kb on + strand at 745.358 kb on + strand at 745.358 kb on + strand at 745.359 kb on - strand at 745.359 kb on - strand at 745.359 kb on - strand at 745.359 kb on - strand at 745.359 kb on - strand at 745.359 kb on - strand at 745.481 kb on + strand at 745.482 kb on - strand at 745.503 kb on + strand at 745.503 kb on + strand at 745.504 kb on - strand at 745.504 kb on - strand at 745.504 kb on - strand at 745.504 kb on - strand at 745.504 kb on - strand at 745.504 kb on - strand at 746.120 kb on + strand at 746.356 kb on + strand, within ABIE53_000692 at 746.356 kb on + strand, within ABIE53_000692 at 746.357 kb on - strand, within ABIE53_000692 at 746.357 kb on - strand, within ABIE53_000692 at 746.357 kb on - strand, within ABIE53_000692 at 746.682 kb on + strand, within ABIE53_000692 at 746.682 kb on + strand, within ABIE53_000692 at 746.683 kb on - strand, within ABIE53_000692 at 746.774 kb on + strand, within ABIE53_000692 at 746.776 kb on + strand, within ABIE53_000692 at 746.776 kb on + strand, within ABIE53_000692 at 746.776 kb on + strand, within ABIE53_000692 at 746.776 kb on + strand, within ABIE53_000692 at 746.776 kb on + strand, within ABIE53_000692 at 746.776 kb on + strand, within ABIE53_000692 at 746.777 kb on - strand, within ABIE53_000692 at 746.777 kb on - strand, within ABIE53_000692 at 746.777 kb on - strand, within ABIE53_000692 at 747.293 kb on + strand, within ABIE53_000692 at 747.294 kb on - strand, within ABIE53_000692 at 747.294 kb on - strand, within ABIE53_000692 at 747.294 kb on - strand, within ABIE53_000692 at 747.294 kb on - strand, within ABIE53_000692 at 747.294 kb on - strand, within ABIE53_000692 at 747.294 kb on - strand, within ABIE53_000692 at 747.294 kb on - strand, within ABIE53_000692 at 747.374 kb on + strand, within ABIE53_000692 at 747.559 kb on + strand, within ABIE53_000692 at 747.559 kb on + strand, within ABIE53_000692 at 747.560 kb on - strand, within ABIE53_000692 at 747.560 kb on - strand, within ABIE53_000692 at 747.704 kb on + strand, within ABIE53_000692 at 747.704 kb on + strand, within ABIE53_000692 at 747.704 kb on + strand, within ABIE53_000692 at 747.704 kb on + strand, within ABIE53_000692 at 747.704 kb on + strand, within ABIE53_000692 at 747.705 kb on - strand, within ABIE53_000692 at 747.705 kb on - strand, within ABIE53_000692 at 747.705 kb on - strand, within ABIE53_000692 at 747.705 kb on - strand, within ABIE53_000692 at 747.705 kb on - strand, within ABIE53_000692 at 747.705 kb on - strand, within ABIE53_000692 at 747.705 kb on - strand, within ABIE53_000692 at 747.705 kb on - strand, within ABIE53_000692 at 747.953 kb on + strand at 747.953 kb on + strand at 747.953 kb on + strand at 747.953 kb on + strand at 747.953 kb on + strand at 748.011 kb on + strand at 748.011 kb on + strand at 748.011 kb on + strand at 748.012 kb on - strand at 748.070 kb on + strand at 748.070 kb on + strand at 748.070 kb on + strand at 748.070 kb on + strand at 748.070 kb on + strand at 748.070 kb on + strand at 748.071 kb on - strand at 748.071 kb on - strand at 748.071 kb on - strand at 748.071 kb on - strand at 748.071 kb on - strand at 748.074 kb on + strand at 748.074 kb on + strand at 748.074 kb on + strand at 748.074 kb on + strand at 748.074 kb on + strand at 748.074 kb on + strand at 748.074 kb on + strand at 748.074 kb on + strand at 748.074 kb on + strand at 748.074 kb on + strand at 748.074 kb on + strand at 748.074 kb on + strand at 748.075 kb on - strand at 748.075 kb on - strand at 748.075 kb on - strand at 748.075 kb on - strand at 748.075 kb on - strand at 748.075 kb on - strand at 748.075 kb on - strand at 748.075 kb on - strand at 748.079 kb on + strand at 748.079 kb on + strand at 748.079 kb on + strand at 748.080 kb on - strand at 748.080 kb on - strand at 748.080 kb on - strand at 748.116 kb on + strand at 748.116 kb on + strand at 748.116 kb on + strand at 748.116 kb on + strand at 748.117 kb on - strand at 748.117 kb on - strand at 748.117 kb on - strand at 748.117 kb on - strand at 748.117 kb on - strand at 748.147 kb on + strand at 748.147 kb on + strand at 748.147 kb on + strand at 748.147 kb on + strand at 748.147 kb on + strand at 748.147 kb on + strand at 748.147 kb on + strand at 748.148 kb on - strand at 748.148 kb on - strand at 748.148 kb on - strand at 748.148 kb on - strand at 748.277 kb on + strand at 748.278 kb on - strand at 748.461 kb on + strand, within ABIE53_000693 at 748.461 kb on + strand, within ABIE53_000693 at 748.461 kb on + strand, within ABIE53_000693 at 748.461 kb on + strand, within ABIE53_000693 at 748.461 kb on + strand, within ABIE53_000693 at 748.461 kb on + strand, within ABIE53_000693 at 748.462 kb on - strand, within ABIE53_000693 at 748.462 kb on - strand, within ABIE53_000693 at 748.462 kb on - strand, within ABIE53_000693 at 748.462 kb on - strand, within ABIE53_000693 at 748.462 kb on - strand, within ABIE53_000693 at 748.462 kb on - strand, within ABIE53_000693 at 748.470 kb on + strand, within ABIE53_000693 at 748.470 kb on + strand, within ABIE53_000693 at 748.470 kb on + strand, within ABIE53_000693 at 748.471 kb on - strand, within ABIE53_000693 at 748.471 kb on - strand, within ABIE53_000693 at 748.471 kb on - strand, within ABIE53_000693 at 748.471 kb on - strand, within ABIE53_000693 at 748.471 kb on - strand, within ABIE53_000693 at 748.494 kb on + strand, within ABIE53_000693 at 748.494 kb on + strand, within ABIE53_000693 at 748.495 kb on - strand, within ABIE53_000693 at 748.495 kb on - strand, within ABIE53_000693 at 748.575 kb on + strand, within ABIE53_000693 at 748.576 kb on - strand, within ABIE53_000693 at 748.576 kb on - strand, within ABIE53_000693 at 748.576 kb on - strand, within ABIE53_000693 at 748.576 kb on - strand, within ABIE53_000693 at 749.001 kb on + strand, within ABIE53_000693 at 749.001 kb on + strand, within ABIE53_000693 at 749.001 kb on + strand, within ABIE53_000693 at 749.001 kb on + strand, within ABIE53_000693 at 749.001 kb on + strand, within ABIE53_000693 at 749.001 kb on + strand, within ABIE53_000693 at 749.001 kb on + strand, within ABIE53_000693 at 749.001 kb on + strand, within ABIE53_000693 at 749.001 kb on + strand, within ABIE53_000693 at 749.001 kb on + strand, within ABIE53_000693 at 749.002 kb on - strand, within ABIE53_000693 at 749.002 kb on - strand, within ABIE53_000693 at 749.002 kb on - strand, within ABIE53_000693 at 749.002 kb on - strand, within ABIE53_000693 at 749.002 kb on - strand, within ABIE53_000693 at 749.002 kb on - strand, within ABIE53_000693 at 749.002 kb on - strand, within ABIE53_000693 at 749.002 kb on - strand, within ABIE53_000693 at 749.002 kb on - strand, within ABIE53_000693
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose_5mM with Epiandrosterone 5 mM remove 745,186 - ABIE53_000690 0.75 +0.9 745,237 + ABIE53_000690 0.80 -0.6 745,358 + +1.0 745,358 + -0.1 745,358 + -0.6 745,358 + +0.7 745,358 + +0.5 745,358 + -0.3 745,359 - -1.3 745,359 - +1.3 745,359 - +0.3 745,359 - -0.8 745,359 - -0.1 745,359 - -1.4 745,481 + -0.6 745,482 - -0.3 745,503 + +2.3 745,503 + +1.3 745,504 - +1.7 745,504 - -0.7 745,504 - -0.1 745,504 - -0.4 745,504 - +0.1 745,504 - -0.2 746,120 + +2.1 746,356 + ABIE53_000692 0.13 -1.4 746,356 + ABIE53_000692 0.13 -0.3 746,357 - ABIE53_000692 0.13 -1.1 746,357 - ABIE53_000692 0.13 +1.5 746,357 - ABIE53_000692 0.13 +0.4 746,682 + ABIE53_000692 0.29 +0.0 746,682 + ABIE53_000692 0.29 -0.3 746,683 - ABIE53_000692 0.29 +0.1 746,774 + ABIE53_000692 0.34 -0.8 746,776 + ABIE53_000692 0.34 -0.6 746,776 + ABIE53_000692 0.34 -0.6 746,776 + ABIE53_000692 0.34 -0.6 746,776 + ABIE53_000692 0.34 +0.0 746,776 + ABIE53_000692 0.34 -0.2 746,776 + ABIE53_000692 0.34 -0.3 746,777 - ABIE53_000692 0.34 -0.7 746,777 - ABIE53_000692 0.34 +1.1 746,777 - ABIE53_000692 0.34 +0.1 747,293 + ABIE53_000692 0.61 -0.9 747,294 - ABIE53_000692 0.61 -0.6 747,294 - ABIE53_000692 0.61 -1.2 747,294 - ABIE53_000692 0.61 -0.3 747,294 - ABIE53_000692 0.61 -1.1 747,294 - ABIE53_000692 0.61 -0.9 747,294 - ABIE53_000692 0.61 -1.1 747,294 - ABIE53_000692 0.61 -1.4 747,374 + ABIE53_000692 0.65 -0.6 747,559 + ABIE53_000692 0.75 +0.1 747,559 + ABIE53_000692 0.75 +1.7 747,560 - ABIE53_000692 0.75 +2.7 747,560 - ABIE53_000692 0.75 -1.7 747,704 + ABIE53_000692 0.82 -1.4 747,704 + ABIE53_000692 0.82 -0.9 747,704 + ABIE53_000692 0.82 -0.4 747,704 + ABIE53_000692 0.82 -1.3 747,704 + ABIE53_000692 0.82 -1.3 747,705 - ABIE53_000692 0.82 +0.5 747,705 - ABIE53_000692 0.82 -0.2 747,705 - ABIE53_000692 0.82 -0.3 747,705 - ABIE53_000692 0.82 -1.1 747,705 - ABIE53_000692 0.82 -0.6 747,705 - ABIE53_000692 0.82 -2.1 747,705 - ABIE53_000692 0.82 +1.4 747,705 - ABIE53_000692 0.82 -0.2 747,953 + -0.1 747,953 + -0.7 747,953 + -1.1 747,953 + -2.0 747,953 + +0.5 748,011 + -1.5 748,011 + -0.5 748,011 + +1.6 748,012 - +1.0 748,070 + -1.2 748,070 + -0.6 748,070 + -0.1 748,070 + -2.2 748,070 + +0.8 748,070 + -1.9 748,071 - -0.3 748,071 - -0.4 748,071 - +0.8 748,071 - -1.1 748,071 - -0.4 748,074 + -0.5 748,074 + -0.5 748,074 + +2.7 748,074 + -0.1 748,074 + -1.2 748,074 + -1.7 748,074 + +0.1 748,074 + -1.3 748,074 + -2.7 748,074 + -0.8 748,074 + -0.6 748,074 + -0.7 748,075 - -0.2 748,075 - -1.2 748,075 - +0.7 748,075 - +0.2 748,075 - +0.5 748,075 - +0.7 748,075 - +0.3 748,075 - -0.7 748,079 + +2.1 748,079 + +0.1 748,079 + -3.1 748,080 - +0.0 748,080 - +0.7 748,080 - -0.9 748,116 + +0.9 748,116 + +1.0 748,116 + +0.1 748,116 + -0.0 748,117 - -1.6 748,117 - +0.9 748,117 - +0.4 748,117 - +2.7 748,117 - +0.9 748,147 + +1.4 748,147 + -1.3 748,147 + -0.4 748,147 + +3.5 748,147 + +0.1 748,147 + +1.7 748,147 + -0.8 748,148 - +0.2 748,148 - -0.3 748,148 - -0.1 748,148 - +1.6 748,277 + -0.9 748,278 - -0.1 748,461 + ABIE53_000693 0.19 +1.2 748,461 + ABIE53_000693 0.19 +1.0 748,461 + ABIE53_000693 0.19 +0.3 748,461 + ABIE53_000693 0.19 +0.2 748,461 + ABIE53_000693 0.19 +1.1 748,461 + ABIE53_000693 0.19 -1.6 748,462 - ABIE53_000693 0.19 +0.1 748,462 - ABIE53_000693 0.19 -0.5 748,462 - ABIE53_000693 0.19 -1.2 748,462 - ABIE53_000693 0.19 -1.7 748,462 - ABIE53_000693 0.19 +2.7 748,462 - ABIE53_000693 0.19 -1.7 748,470 + ABIE53_000693 0.20 -1.2 748,470 + ABIE53_000693 0.20 +0.6 748,470 + ABIE53_000693 0.20 +0.2 748,471 - ABIE53_000693 0.20 -0.9 748,471 - ABIE53_000693 0.20 -0.3 748,471 - ABIE53_000693 0.20 +0.1 748,471 - ABIE53_000693 0.20 -0.1 748,471 - ABIE53_000693 0.20 +0.1 748,494 + ABIE53_000693 0.21 -0.1 748,494 + ABIE53_000693 0.21 +1.5 748,495 - ABIE53_000693 0.21 +1.4 748,495 - ABIE53_000693 0.21 +1.1 748,575 + ABIE53_000693 0.26 +0.6 748,576 - ABIE53_000693 0.26 -0.3 748,576 - ABIE53_000693 0.26 -0.0 748,576 - ABIE53_000693 0.26 +2.7 748,576 - ABIE53_000693 0.26 +0.0 749,001 + ABIE53_000693 0.49 -0.2 749,001 + ABIE53_000693 0.49 +0.1 749,001 + ABIE53_000693 0.49 -0.9 749,001 + ABIE53_000693 0.49 +0.3 749,001 + ABIE53_000693 0.49 -0.6 749,001 + ABIE53_000693 0.49 -0.7 749,001 + ABIE53_000693 0.49 -0.8 749,001 + ABIE53_000693 0.49 -0.3 749,001 + ABIE53_000693 0.49 -1.3 749,001 + ABIE53_000693 0.49 +0.7 749,002 - ABIE53_000693 0.50 +0.7 749,002 - ABIE53_000693 0.50 -1.4 749,002 - ABIE53_000693 0.50 -1.0 749,002 - ABIE53_000693 0.50 -1.3 749,002 - ABIE53_000693 0.50 +0.5 749,002 - ABIE53_000693 0.50 -0.7 749,002 - ABIE53_000693 0.50 -0.9 749,002 - ABIE53_000693 0.50 -0.7 749,002 - ABIE53_000693 0.50 +1.7
Or see this region's nucleotide sequence