Experiment: MoYLS4 with Polyethylene glycol 5%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nifD and glnB-2 are separated by 11 nucleotides glnB-2 and glnB-3 are separated by 9 nucleotides glnB-3 and nifH are separated by 282 nucleotides
DVUA0012: nifD - nitrogenase molybdenum-iron protein alpha chain (TIGR), at 16,223 to 17,851
nifD
DVUA0013: glnB-2 - nitrogen regulatory protein P-II (TIGR), at 17,863 to 18,258
glnB-2
DVUA0014: glnB-3 - nitrogen regulatory protein P-II (TIGR), at 18,268 to 18,594
glnB-3
DVUA0015: nifH - nitrogenase iron protein (TIGR), at 18,877 to 19,710
nifH
Position (kb) 17
18
19 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 16.866 kb on - strand, within nifD at 16.920 kb on + strand, within nifD at 16.923 kb on - strand, within nifD at 16.928 kb on - strand, within nifD at 16.994 kb on - strand, within nifD at 17.040 kb on + strand, within nifD at 17.064 kb on + strand, within nifD at 17.072 kb on - strand, within nifD at 17.100 kb on + strand, within nifD at 17.131 kb on - strand, within nifD at 17.173 kb on - strand, within nifD at 17.250 kb on + strand, within nifD at 17.282 kb on + strand, within nifD at 17.288 kb on - strand, within nifD at 17.295 kb on + strand, within nifD at 17.305 kb on - strand, within nifD at 17.366 kb on + strand, within nifD at 17.405 kb on - strand, within nifD at 17.438 kb on + strand, within nifD at 17.557 kb on + strand, within nifD at 17.600 kb on + strand, within nifD at 17.655 kb on - strand, within nifD at 17.668 kb on + strand, within nifD at 17.668 kb on + strand, within nifD at 17.694 kb on + strand at 17.701 kb on - strand at 17.786 kb on + strand at 17.831 kb on - strand at 17.843 kb on - strand at 17.919 kb on - strand, within glnB-2 at 17.997 kb on + strand, within glnB-2 at 18.002 kb on + strand, within glnB-2 at 18.061 kb on - strand, within glnB-2 at 18.085 kb on - strand, within glnB-2 at 18.186 kb on + strand, within glnB-2 at 18.242 kb on - strand at 18.282 kb on + strand at 18.296 kb on + strand at 18.296 kb on + strand at 18.296 kb on + strand at 18.304 kb on - strand, within glnB-3 at 18.304 kb on - strand, within glnB-3 at 18.304 kb on - strand, within glnB-3 at 18.304 kb on - strand, within glnB-3 at 18.353 kb on + strand, within glnB-3 at 18.370 kb on + strand, within glnB-3 at 18.378 kb on - strand, within glnB-3 at 18.404 kb on + strand, within glnB-3 at 18.404 kb on + strand, within glnB-3 at 18.431 kb on + strand, within glnB-3 at 18.439 kb on - strand, within glnB-3 at 18.439 kb on - strand, within glnB-3 at 18.466 kb on - strand, within glnB-3 at 18.473 kb on - strand, within glnB-3 at 18.492 kb on - strand, within glnB-3 at 18.506 kb on - strand, within glnB-3 at 18.527 kb on + strand, within glnB-3 at 18.535 kb on - strand, within glnB-3 at 18.559 kb on - strand, within glnB-3 at 18.747 kb on + strand at 18.755 kb on - strand at 18.849 kb on - strand at 18.884 kb on + strand at 18.922 kb on - strand at 18.994 kb on - strand, within nifH at 19.054 kb on + strand, within nifH
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polyethylene glycol 5% remove 16,866 - nifD DVUA0012 0.39 -0.5 16,920 + nifD DVUA0012 0.43 +0.9 16,923 - nifD DVUA0012 0.43 +0.7 16,928 - nifD DVUA0012 0.43 +0.9 16,994 - nifD DVUA0012 0.47 +0.6 17,040 + nifD DVUA0012 0.50 +3.2 17,064 + nifD DVUA0012 0.52 -1.1 17,072 - nifD DVUA0012 0.52 -1.1 17,100 + nifD DVUA0012 0.54 -0.3 17,131 - nifD DVUA0012 0.56 -0.3 17,173 - nifD DVUA0012 0.58 +0.1 17,250 + nifD DVUA0012 0.63 -0.7 17,282 + nifD DVUA0012 0.65 -0.3 17,288 - nifD DVUA0012 0.65 +0.7 17,295 + nifD DVUA0012 0.66 +0.0 17,305 - nifD DVUA0012 0.66 -0.2 17,366 + nifD DVUA0012 0.70 -0.6 17,405 - nifD DVUA0012 0.73 -0.3 17,438 + nifD DVUA0012 0.75 -0.3 17,557 + nifD DVUA0012 0.82 +0.0 17,600 + nifD DVUA0012 0.85 +0.2 17,655 - nifD DVUA0012 0.88 -0.1 17,668 + nifD DVUA0012 0.89 +0.8 17,668 + nifD DVUA0012 0.89 -0.5 17,694 + +0.2 17,701 - -0.2 17,786 + -0.1 17,831 - +0.3 17,843 - +0.7 17,919 - glnB-2 DVUA0013 0.14 -1.8 17,997 + glnB-2 DVUA0013 0.34 -0.4 18,002 + glnB-2 DVUA0013 0.35 -0.8 18,061 - glnB-2 DVUA0013 0.50 -0.8 18,085 - glnB-2 DVUA0013 0.56 +1.8 18,186 + glnB-2 DVUA0013 0.82 -0.8 18,242 - +0.2 18,282 + -0.2 18,296 + +0.2 18,296 + +0.5 18,296 + +0.7 18,304 - glnB-3 DVUA0014 0.11 +0.9 18,304 - glnB-3 DVUA0014 0.11 -0.1 18,304 - glnB-3 DVUA0014 0.11 +1.2 18,304 - glnB-3 DVUA0014 0.11 +0.7 18,353 + glnB-3 DVUA0014 0.26 -0.6 18,370 + glnB-3 DVUA0014 0.31 +0.5 18,378 - glnB-3 DVUA0014 0.34 +1.1 18,404 + glnB-3 DVUA0014 0.42 -0.8 18,404 + glnB-3 DVUA0014 0.42 -0.6 18,431 + glnB-3 DVUA0014 0.50 -0.2 18,439 - glnB-3 DVUA0014 0.52 -1.1 18,439 - glnB-3 DVUA0014 0.52 +0.3 18,466 - glnB-3 DVUA0014 0.61 -0.5 18,473 - glnB-3 DVUA0014 0.63 -1.1 18,492 - glnB-3 DVUA0014 0.69 -0.5 18,506 - glnB-3 DVUA0014 0.73 -0.8 18,527 + glnB-3 DVUA0014 0.79 -0.8 18,535 - glnB-3 DVUA0014 0.82 +0.0 18,559 - glnB-3 DVUA0014 0.89 -0.1 18,747 + -0.2 18,755 - +0.1 18,849 - -0.2 18,884 + -0.2 18,922 - +0.1 18,994 - nifH DVUA0015 0.14 -0.8 19,054 + nifH DVUA0015 0.21 +0.1
Or see this region's nucleotide sequence