Experiment: MoYLS4 with Polyethylene glycol 5%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1525 and DVU1526 are separated by 363 nucleotides DVU1526 and DVU1527 overlap by 4 nucleotides DVU1527 and tRNA-Ser are separated by 176 nucleotides tRNA-Ser and DVU1528 are separated by 342 nucleotides
DVU1525: DVU1525 - conserved domain protein (TIGR), at 1,593,138 to 1,594,130
DVU1525
DVU1526: DVU1526 - hypothetical protein (TIGR), at 1,594,494 to 1,594,712
DVU1526
DVU1527: DVU1527 - site-specific recombinase, phage integrase family (TIGR), at 1,594,709 to 1,595,902
DVU1527
DVU_tRNA-Ser-4: tRNA-Ser - tRNA with anticodon CGA for Ser (VIMSS-AUTO), at 1,596,079 to 1,596,173
tRNA-Ser
DVU1528: DVU1528 - cytidine/deoxycytidylate deaminase family protein (TIGR), at 1,596,516 to 1,597,001
DVU1528
Position (kb)
1594
1595
1596 Strain fitness (log2 ratio)
-1
0
1
2 at 1593.712 kb on + strand, within DVU1525 at 1593.712 kb on - strand, within DVU1525 at 1593.725 kb on - strand, within DVU1525 at 1593.766 kb on + strand, within DVU1525 at 1593.854 kb on + strand, within DVU1525 at 1593.905 kb on + strand, within DVU1525 at 1593.907 kb on + strand, within DVU1525 at 1593.934 kb on - strand, within DVU1525 at 1593.945 kb on + strand, within DVU1525 at 1593.955 kb on + strand, within DVU1525 at 1593.971 kb on + strand, within DVU1525 at 1593.972 kb on + strand, within DVU1525 at 1593.991 kb on + strand, within DVU1525 at 1594.055 kb on + strand at 1594.136 kb on + strand at 1594.139 kb on + strand at 1594.183 kb on + strand at 1594.191 kb on - strand at 1594.225 kb on - strand at 1594.234 kb on + strand at 1594.242 kb on - strand at 1594.242 kb on - strand at 1594.312 kb on - strand at 1594.313 kb on + strand at 1594.392 kb on + strand at 1594.416 kb on + strand at 1594.417 kb on - strand at 1594.446 kb on - strand at 1594.446 kb on - strand at 1594.568 kb on + strand, within DVU1526 at 1594.580 kb on - strand, within DVU1526 at 1594.586 kb on - strand, within DVU1526 at 1594.586 kb on - strand, within DVU1526 at 1594.666 kb on - strand, within DVU1526 at 1594.668 kb on - strand, within DVU1526 at 1594.707 kb on - strand at 1594.720 kb on - strand at 1594.760 kb on - strand at 1594.780 kb on + strand at 1594.824 kb on + strand at 1594.832 kb on - strand, within DVU1527 at 1595.014 kb on + strand, within DVU1527 at 1595.014 kb on + strand, within DVU1527 at 1595.054 kb on + strand, within DVU1527 at 1595.072 kb on - strand, within DVU1527 at 1595.093 kb on - strand, within DVU1527 at 1595.130 kb on + strand, within DVU1527 at 1595.206 kb on - strand, within DVU1527 at 1595.233 kb on - strand, within DVU1527 at 1595.272 kb on - strand, within DVU1527 at 1595.403 kb on - strand, within DVU1527 at 1595.403 kb on - strand, within DVU1527 at 1595.531 kb on - strand, within DVU1527 at 1595.587 kb on + strand, within DVU1527 at 1595.818 kb on + strand at 1595.826 kb on - strand at 1595.883 kb on + strand at 1595.891 kb on - strand at 1595.925 kb on - strand at 1595.958 kb on + strand at 1596.343 kb on + strand at 1596.343 kb on + strand at 1596.358 kb on - strand at 1596.371 kb on - strand at 1596.378 kb on + strand at 1596.403 kb on - strand at 1596.405 kb on + strand at 1596.405 kb on + strand at 1596.499 kb on + strand at 1596.499 kb on + strand at 1596.507 kb on - strand at 1596.507 kb on - strand at 1596.523 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polyethylene glycol 5% remove 1,593,712 + DVU1525 0.58 +0.2 1,593,712 - DVU1525 0.58 +0.3 1,593,725 - DVU1525 0.59 -0.3 1,593,766 + DVU1525 0.63 -0.1 1,593,854 + DVU1525 0.72 -0.4 1,593,905 + DVU1525 0.77 +0.0 1,593,907 + DVU1525 0.77 -0.6 1,593,934 - DVU1525 0.80 +1.1 1,593,945 + DVU1525 0.81 +0.3 1,593,955 + DVU1525 0.82 -0.5 1,593,971 + DVU1525 0.84 -1.4 1,593,972 + DVU1525 0.84 -0.5 1,593,991 + DVU1525 0.86 +1.1 1,594,055 + -0.4 1,594,136 + -0.8 1,594,139 + +0.9 1,594,183 + +0.7 1,594,191 - -0.4 1,594,225 - -0.2 1,594,234 + +0.7 1,594,242 - -0.1 1,594,242 - -0.6 1,594,312 - -0.8 1,594,313 + +0.4 1,594,392 + -0.2 1,594,416 + +2.0 1,594,417 - +1.7 1,594,446 - -0.4 1,594,446 - -0.3 1,594,568 + DVU1526 0.34 +0.2 1,594,580 - DVU1526 0.39 -0.2 1,594,586 - DVU1526 0.42 -0.4 1,594,586 - DVU1526 0.42 -0.5 1,594,666 - DVU1526 0.79 -0.4 1,594,668 - DVU1526 0.79 +0.2 1,594,707 - +0.0 1,594,720 - -1.4 1,594,760 - -0.6 1,594,780 + +0.8 1,594,824 + +0.4 1,594,832 - DVU1527 0.10 -1.1 1,595,014 + DVU1527 0.26 -1.2 1,595,014 + DVU1527 0.26 -0.7 1,595,054 + DVU1527 0.29 +0.1 1,595,072 - DVU1527 0.30 +0.2 1,595,093 - DVU1527 0.32 +0.6 1,595,130 + DVU1527 0.35 -0.2 1,595,206 - DVU1527 0.42 -0.2 1,595,233 - DVU1527 0.44 +0.2 1,595,272 - DVU1527 0.47 -1.6 1,595,403 - DVU1527 0.58 -0.4 1,595,403 - DVU1527 0.58 -0.1 1,595,531 - DVU1527 0.69 -0.4 1,595,587 + DVU1527 0.74 -1.2 1,595,818 + -0.6 1,595,826 - -0.2 1,595,883 + -1.3 1,595,891 - +0.0 1,595,925 - +0.3 1,595,958 + -0.2 1,596,343 + -1.0 1,596,343 + -1.2 1,596,358 - -0.2 1,596,371 - -0.4 1,596,378 + +0.9 1,596,403 - -1.1 1,596,405 + -0.7 1,596,405 + -0.4 1,596,499 + +0.4 1,596,499 + -0.3 1,596,507 - -0.1 1,596,507 - -0.2 1,596,523 + +0.1
Or see this region's nucleotide sequence