Experiment: MoYLS4 with Polyethylene glycol 5%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1082 and phoB are separated by 173 nucleotides phoB and pstB-1 are separated by 27 nucleotides pstB-1 and phoU are separated by 8 nucleotides
DVU1082: DVU1082 - 3- 5 exonuclease domain protein (TIGR), at 1,186,345 to 1,186,953
DVU1082
DVU1083: phoB - phosphate regulon transcriptional regulator PhoB (TIGR), at 1,187,127 to 1,187,816
phoB
DVU1084: pstB-1 - phosphate ABC transporter, ATP-binding protein (TIGR), at 1,187,844 to 1,188,611
pstB-1
DVU1085: phoU - phosphate transport system protein PhoU (TIGR), at 1,188,620 to 1,189,285
phoU
Position (kb)
1187
1188
1189 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1186.858 kb on - strand, within DVU1082 at 1187.052 kb on + strand at 1187.064 kb on + strand at 1187.118 kb on + strand at 1187.873 kb on + strand at 1187.923 kb on - strand, within pstB-1 at 1188.001 kb on + strand, within pstB-1 at 1188.071 kb on + strand, within pstB-1 at 1188.134 kb on + strand, within pstB-1 at 1188.183 kb on + strand, within pstB-1 at 1188.201 kb on - strand, within pstB-1 at 1188.228 kb on + strand, within pstB-1 at 1188.251 kb on + strand, within pstB-1 at 1188.257 kb on - strand, within pstB-1 at 1188.259 kb on - strand, within pstB-1 at 1188.276 kb on + strand, within pstB-1 at 1188.276 kb on + strand, within pstB-1 at 1188.284 kb on - strand, within pstB-1 at 1188.320 kb on - strand, within pstB-1 at 1188.370 kb on - strand, within pstB-1 at 1188.517 kb on - strand, within pstB-1 at 1188.548 kb on + strand at 1188.572 kb on + strand at 1188.595 kb on - strand at 1188.716 kb on + strand, within phoU at 1188.807 kb on + strand, within phoU at 1188.867 kb on + strand, within phoU at 1188.875 kb on - strand, within phoU at 1188.980 kb on - strand, within phoU at 1188.981 kb on + strand, within phoU at 1189.016 kb on + strand, within phoU at 1189.024 kb on - strand, within phoU at 1189.193 kb on - strand, within phoU at 1189.280 kb on + strand at 1189.336 kb on - strand at 1189.390 kb on - strand at 1189.514 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polyethylene glycol 5% remove 1,186,858 - DVU1082 0.84 +0.3 1,187,052 + -0.4 1,187,064 + -2.0 1,187,118 + +0.8 1,187,873 + +0.5 1,187,923 - pstB-1 DVU1084 0.10 +1.4 1,188,001 + pstB-1 DVU1084 0.20 -1.0 1,188,071 + pstB-1 DVU1084 0.30 +0.1 1,188,134 + pstB-1 DVU1084 0.38 +0.4 1,188,183 + pstB-1 DVU1084 0.44 -0.1 1,188,201 - pstB-1 DVU1084 0.46 +1.1 1,188,228 + pstB-1 DVU1084 0.50 +0.1 1,188,251 + pstB-1 DVU1084 0.53 -0.1 1,188,257 - pstB-1 DVU1084 0.54 +0.8 1,188,259 - pstB-1 DVU1084 0.54 -1.2 1,188,276 + pstB-1 DVU1084 0.56 +0.1 1,188,276 + pstB-1 DVU1084 0.56 -0.8 1,188,284 - pstB-1 DVU1084 0.57 +0.2 1,188,320 - pstB-1 DVU1084 0.62 +1.8 1,188,370 - pstB-1 DVU1084 0.68 +0.9 1,188,517 - pstB-1 DVU1084 0.88 +0.9 1,188,548 + -0.2 1,188,572 + +0.2 1,188,595 - +0.5 1,188,716 + phoU DVU1085 0.14 +0.2 1,188,807 + phoU DVU1085 0.28 -1.1 1,188,867 + phoU DVU1085 0.37 -0.4 1,188,875 - phoU DVU1085 0.38 +0.4 1,188,980 - phoU DVU1085 0.54 +1.3 1,188,981 + phoU DVU1085 0.54 -2.0 1,189,016 + phoU DVU1085 0.59 +0.8 1,189,024 - phoU DVU1085 0.61 -0.1 1,189,193 - phoU DVU1085 0.86 -0.2 1,189,280 + +0.4 1,189,336 - +1.0 1,189,390 - -0.2 1,189,514 - -0.4
Or see this region's nucleotide sequence