Experiment: RCH2_defined_glucose_5mM with Epiandrosterone 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_000221 and ABIE53_000222 overlap by 8 nucleotides ABIE53_000222 and ABIE53_000223 are separated by 959 nucleotides
ABIE53_000220: ABIE53_000220 - hypothetical protein, at 225,008 to 225,331
_000220
ABIE53_000221: ABIE53_000221 - hypothetical protein, at 225,315 to 226,325
_000221
ABIE53_000222: ABIE53_000222 - hypothetical protein, at 226,318 to 226,605
_000222
ABIE53_000223: ABIE53_000223 - hypothetical protein, at 227,565 to 227,804
_000223
Position (kb) 226
227 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 225.362 kb on + strand at 225.362 kb on + strand at 225.362 kb on + strand at 225.369 kb on - strand at 225.369 kb on - strand at 225.369 kb on - strand at 225.369 kb on - strand at 225.369 kb on - strand at 225.369 kb on - strand at 225.408 kb on - strand at 225.531 kb on - strand, within ABIE53_000221 at 225.572 kb on - strand, within ABIE53_000221 at 225.572 kb on - strand, within ABIE53_000221 at 225.579 kb on + strand, within ABIE53_000221 at 225.580 kb on - strand, within ABIE53_000221 at 225.580 kb on - strand, within ABIE53_000221 at 225.580 kb on - strand, within ABIE53_000221 at 225.580 kb on - strand, within ABIE53_000221 at 225.647 kb on - strand, within ABIE53_000221 at 225.691 kb on + strand, within ABIE53_000221 at 225.691 kb on + strand, within ABIE53_000221 at 225.692 kb on - strand, within ABIE53_000221 at 225.692 kb on - strand, within ABIE53_000221 at 225.692 kb on - strand, within ABIE53_000221 at 225.747 kb on + strand, within ABIE53_000221 at 225.858 kb on + strand, within ABIE53_000221 at 225.858 kb on + strand, within ABIE53_000221 at 225.859 kb on - strand, within ABIE53_000221 at 225.859 kb on - strand, within ABIE53_000221 at 225.859 kb on - strand, within ABIE53_000221 at 225.925 kb on + strand, within ABIE53_000221 at 225.925 kb on + strand, within ABIE53_000221 at 225.926 kb on - strand, within ABIE53_000221 at 225.926 kb on - strand, within ABIE53_000221 at 225.926 kb on - strand, within ABIE53_000221 at 225.926 kb on - strand, within ABIE53_000221 at 225.926 kb on - strand, within ABIE53_000221 at 225.970 kb on - strand, within ABIE53_000221 at 226.029 kb on + strand, within ABIE53_000221 at 226.080 kb on + strand, within ABIE53_000221 at 226.080 kb on + strand, within ABIE53_000221 at 226.080 kb on + strand, within ABIE53_000221 at 226.081 kb on - strand, within ABIE53_000221 at 226.081 kb on - strand, within ABIE53_000221 at 226.132 kb on + strand, within ABIE53_000221 at 226.132 kb on + strand, within ABIE53_000221 at 226.132 kb on + strand, within ABIE53_000221 at 226.132 kb on + strand, within ABIE53_000221 at 226.132 kb on + strand, within ABIE53_000221 at 226.133 kb on - strand, within ABIE53_000221 at 226.133 kb on - strand, within ABIE53_000221 at 226.143 kb on + strand, within ABIE53_000221 at 226.144 kb on - strand, within ABIE53_000221 at 226.257 kb on + strand at 226.257 kb on + strand at 226.258 kb on - strand at 226.276 kb on + strand at 226.276 kb on + strand at 226.276 kb on + strand at 226.276 kb on + strand at 226.277 kb on - strand at 226.277 kb on - strand at 226.281 kb on - strand at 226.320 kb on - strand at 226.351 kb on + strand, within ABIE53_000222 at 226.351 kb on + strand, within ABIE53_000222 at 226.352 kb on - strand, within ABIE53_000222 at 226.352 kb on - strand, within ABIE53_000222 at 226.352 kb on - strand, within ABIE53_000222 at 226.404 kb on + strand, within ABIE53_000222 at 226.483 kb on + strand, within ABIE53_000222 at 226.483 kb on + strand, within ABIE53_000222 at 226.483 kb on + strand, within ABIE53_000222 at 226.483 kb on + strand, within ABIE53_000222 at 226.483 kb on + strand, within ABIE53_000222 at 226.628 kb on + strand at 226.673 kb on + strand at 226.673 kb on + strand at 226.673 kb on + strand at 226.674 kb on - strand at 226.674 kb on - strand at 226.674 kb on - strand at 226.694 kb on + strand at 226.694 kb on + strand at 226.694 kb on + strand at 226.694 kb on + strand at 226.694 kb on + strand at 226.694 kb on + strand at 226.694 kb on + strand at 226.695 kb on - strand at 226.695 kb on - strand at 226.695 kb on - strand at 226.695 kb on - strand at 226.695 kb on - strand at 226.695 kb on - strand at 226.772 kb on - strand at 226.796 kb on + strand at 226.797 kb on - strand at 226.806 kb on + strand at 226.807 kb on - strand at 226.807 kb on - strand at 226.807 kb on - strand at 226.835 kb on + strand at 226.835 kb on + strand at 226.835 kb on + strand at 226.835 kb on + strand at 226.835 kb on + strand at 226.836 kb on - strand at 226.836 kb on - strand at 226.838 kb on + strand at 226.838 kb on + strand at 226.839 kb on - strand at 226.839 kb on - strand at 226.878 kb on + strand at 226.879 kb on - strand at 226.879 kb on - strand at 226.929 kb on + strand at 226.930 kb on - strand at 226.930 kb on - strand at 226.965 kb on + strand at 227.046 kb on + strand at 227.047 kb on - strand at 227.047 kb on - strand at 227.107 kb on + strand at 227.108 kb on - strand at 227.108 kb on - strand at 227.114 kb on + strand at 227.114 kb on + strand at 227.114 kb on + strand at 227.115 kb on - strand at 227.115 kb on - strand at 227.115 kb on - strand at 227.156 kb on - strand at 227.156 kb on - strand at 227.156 kb on - strand at 227.166 kb on - strand at 227.166 kb on - strand at 227.169 kb on + strand at 227.169 kb on + strand at 227.169 kb on + strand at 227.169 kb on + strand at 227.169 kb on + strand at 227.170 kb on - strand at 227.170 kb on - strand at 227.170 kb on - strand at 227.170 kb on - strand at 227.285 kb on + strand at 227.285 kb on + strand at 227.285 kb on + strand at 227.285 kb on + strand at 227.285 kb on + strand at 227.285 kb on + strand at 227.285 kb on + strand at 227.285 kb on + strand at 227.285 kb on + strand at 227.286 kb on - strand at 227.286 kb on - strand at 227.286 kb on - strand at 227.286 kb on - strand at 227.286 kb on - strand at 227.286 kb on - strand at 227.286 kb on - strand at 227.286 kb on - strand at 227.286 kb on - strand at 227.286 kb on - strand at 227.333 kb on + strand at 227.333 kb on + strand at 227.334 kb on - strand at 227.334 kb on - strand at 227.371 kb on + strand at 227.371 kb on + strand at 227.372 kb on - strand at 227.372 kb on - strand at 227.416 kb on + strand at 227.417 kb on - strand at 227.417 kb on - strand at 227.460 kb on + strand at 227.460 kb on + strand at 227.461 kb on - strand at 227.468 kb on + strand at 227.468 kb on + strand at 227.468 kb on + strand at 227.468 kb on + strand at 227.468 kb on + strand at 227.469 kb on - strand at 227.469 kb on - strand at 227.514 kb on + strand at 227.515 kb on - strand at 227.559 kb on + strand at 227.559 kb on + strand at 227.559 kb on + strand at 227.559 kb on + strand at 227.560 kb on - strand at 227.560 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose_5mM with Epiandrosterone 5 mM remove 225,362 + +0.3 225,362 + -0.5 225,362 + -2.6 225,369 - -0.8 225,369 - -0.4 225,369 - -0.8 225,369 - -0.8 225,369 - +0.5 225,369 - -1.0 225,408 - -1.5 225,531 - ABIE53_000221 0.21 -2.0 225,572 - ABIE53_000221 0.25 +1.2 225,572 - ABIE53_000221 0.25 +0.5 225,579 + ABIE53_000221 0.26 +1.2 225,580 - ABIE53_000221 0.26 -0.4 225,580 - ABIE53_000221 0.26 +0.2 225,580 - ABIE53_000221 0.26 +0.9 225,580 - ABIE53_000221 0.26 -0.0 225,647 - ABIE53_000221 0.33 -2.5 225,691 + ABIE53_000221 0.37 +0.5 225,691 + ABIE53_000221 0.37 -1.7 225,692 - ABIE53_000221 0.37 -0.2 225,692 - ABIE53_000221 0.37 +2.0 225,692 - ABIE53_000221 0.37 -0.1 225,747 + ABIE53_000221 0.43 +3.3 225,858 + ABIE53_000221 0.54 -1.5 225,858 + ABIE53_000221 0.54 +2.0 225,859 - ABIE53_000221 0.54 -0.2 225,859 - ABIE53_000221 0.54 +1.5 225,859 - ABIE53_000221 0.54 +0.4 225,925 + ABIE53_000221 0.60 -1.1 225,925 + ABIE53_000221 0.60 -0.5 225,926 - ABIE53_000221 0.60 -1.2 225,926 - ABIE53_000221 0.60 -1.1 225,926 - ABIE53_000221 0.60 +0.4 225,926 - ABIE53_000221 0.60 -0.5 225,926 - ABIE53_000221 0.60 +0.1 225,970 - ABIE53_000221 0.65 -0.7 226,029 + ABIE53_000221 0.71 +0.5 226,080 + ABIE53_000221 0.76 +0.6 226,080 + ABIE53_000221 0.76 -1.9 226,080 + ABIE53_000221 0.76 +0.6 226,081 - ABIE53_000221 0.76 -0.3 226,081 - ABIE53_000221 0.76 +4.0 226,132 + ABIE53_000221 0.81 -0.1 226,132 + ABIE53_000221 0.81 -0.5 226,132 + ABIE53_000221 0.81 -1.8 226,132 + ABIE53_000221 0.81 -0.0 226,132 + ABIE53_000221 0.81 +0.4 226,133 - ABIE53_000221 0.81 +1.0 226,133 - ABIE53_000221 0.81 -1.1 226,143 + ABIE53_000221 0.82 +1.3 226,144 - ABIE53_000221 0.82 -0.2 226,257 + -0.6 226,257 + -0.9 226,258 - -0.6 226,276 + -0.8 226,276 + -1.0 226,276 + +0.1 226,276 + -0.6 226,277 - +1.0 226,277 - -0.6 226,281 - -0.8 226,320 - +1.7 226,351 + ABIE53_000222 0.11 -0.8 226,351 + ABIE53_000222 0.11 -1.1 226,352 - ABIE53_000222 0.12 -0.8 226,352 - ABIE53_000222 0.12 +0.9 226,352 - ABIE53_000222 0.12 -3.2 226,404 + ABIE53_000222 0.30 -0.0 226,483 + ABIE53_000222 0.57 -1.2 226,483 + ABIE53_000222 0.57 -0.5 226,483 + ABIE53_000222 0.57 -0.6 226,483 + ABIE53_000222 0.57 -0.1 226,483 + ABIE53_000222 0.57 -0.8 226,628 + -0.6 226,673 + -1.0 226,673 + +1.4 226,673 + +0.2 226,674 - -0.6 226,674 - +1.2 226,674 - -0.2 226,694 + -0.1 226,694 + -0.8 226,694 + +1.0 226,694 + +0.1 226,694 + +0.5 226,694 + +0.5 226,694 + -0.6 226,695 - +2.7 226,695 - -1.6 226,695 - +1.5 226,695 - +0.6 226,695 - +0.2 226,695 - +1.0 226,772 - -1.0 226,796 + -0.8 226,797 - +0.3 226,806 + -0.2 226,807 - +0.2 226,807 - -0.6 226,807 - -0.8 226,835 + -3.3 226,835 + -0.6 226,835 + +0.1 226,835 + -1.3 226,835 + -0.5 226,836 - -1.0 226,836 - -1.4 226,838 + -2.7 226,838 + -0.8 226,839 - -0.0 226,839 - -0.4 226,878 + -0.2 226,879 - -0.2 226,879 - +0.7 226,929 + -1.6 226,930 - -0.6 226,930 - +0.7 226,965 + -2.0 227,046 + -1.1 227,047 - +0.5 227,047 - +0.1 227,107 + -0.8 227,108 - +0.6 227,108 - -0.5 227,114 + +0.4 227,114 + -2.3 227,114 + -1.2 227,115 - +1.5 227,115 - -1.3 227,115 - +2.0 227,156 - -0.8 227,156 - +0.2 227,156 - -1.8 227,166 - +0.6 227,166 - +0.3 227,169 + -0.5 227,169 + +0.3 227,169 + -1.0 227,169 + -0.5 227,169 + -0.9 227,170 - +0.6 227,170 - +1.0 227,170 - -1.2 227,170 - -0.5 227,285 + +0.6 227,285 + -1.1 227,285 + -0.7 227,285 + -1.5 227,285 + -0.3 227,285 + +0.5 227,285 + +0.1 227,285 + -0.0 227,285 + -0.9 227,286 - -0.0 227,286 - -0.2 227,286 - +1.4 227,286 - +1.0 227,286 - -0.7 227,286 - +1.6 227,286 - -0.6 227,286 - +0.5 227,286 - +1.2 227,286 - -0.9 227,333 + -0.5 227,333 + +0.5 227,334 - -1.5 227,334 - -0.7 227,371 + +1.1 227,371 + -0.2 227,372 - +0.9 227,372 - +0.2 227,416 + -0.5 227,417 - -1.2 227,417 - -1.9 227,460 + -1.4 227,460 + +2.0 227,461 - +0.4 227,468 + +1.0 227,468 + -1.1 227,468 + +0.1 227,468 + -1.1 227,468 + -1.3 227,469 - -0.8 227,469 - -0.4 227,514 + -0.8 227,515 - -0.8 227,559 + +0.5 227,559 + +0.7 227,559 + -1.0 227,559 + +0.4 227,560 - +1.0 227,560 - -0.5
Or see this region's nucleotide sequence