Experiment: MoYLS4 with Polyethylene glycol 5%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt amt and glnB-1 are separated by 122 nucleotides glnB-1 and glnD are separated by 55 nucleotides glnD and DVU1234 are separated by 70 nucleotides
DVU1231: amt - ammonium transporter (TIGR), at 1,318,601 to 1,319,809
amt
DVU1232: glnB-1 - nitrogen regulatory protein P-II (TIGR), at 1,319,932 to 1,320,270
glnB-1
DVU1233: glnD - protein-P-II uridylyltransferase, putative (TIGR), at 1,320,326 to 1,323,046
glnD
DVU1234: DVU1234 - membrane protein, putative (TIGR), at 1,323,117 to 1,323,848
DVU1234
Position (kb)
1320
1321
1322
1323
1324 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1319.361 kb on + strand, within amt at 1319.368 kb on + strand, within amt at 1319.564 kb on - strand, within amt at 1319.597 kb on + strand, within amt at 1319.605 kb on - strand, within amt at 1319.615 kb on + strand, within amt at 1319.653 kb on + strand, within amt at 1319.670 kb on + strand, within amt at 1319.683 kb on + strand, within amt at 1319.707 kb on + strand at 1319.715 kb on - strand at 1319.820 kb on - strand at 1319.883 kb on - strand at 1319.902 kb on + strand at 1319.924 kb on - strand at 1319.980 kb on + strand, within glnB-1 at 1320.057 kb on + strand, within glnB-1 at 1320.105 kb on + strand, within glnB-1 at 1320.105 kb on + strand, within glnB-1 at 1320.138 kb on + strand, within glnB-1 at 1320.146 kb on - strand, within glnB-1 at 1320.146 kb on - strand, within glnB-1 at 1320.162 kb on + strand, within glnB-1 at 1320.168 kb on - strand, within glnB-1 at 1320.379 kb on + strand at 1320.483 kb on - strand at 1320.506 kb on - strand at 1320.508 kb on + strand at 1320.518 kb on + strand at 1320.518 kb on + strand at 1320.518 kb on + strand at 1320.526 kb on - strand at 1320.568 kb on + strand at 1320.568 kb on + strand at 1320.568 kb on + strand at 1320.660 kb on - strand, within glnD at 1320.728 kb on + strand, within glnD at 1320.743 kb on + strand, within glnD at 1320.791 kb on - strand, within glnD at 1320.813 kb on - strand, within glnD at 1320.814 kb on - strand, within glnD at 1320.821 kb on - strand, within glnD at 1320.929 kb on + strand, within glnD at 1320.938 kb on + strand, within glnD at 1320.938 kb on + strand, within glnD at 1321.016 kb on - strand, within glnD at 1321.060 kb on - strand, within glnD at 1321.266 kb on + strand, within glnD at 1321.365 kb on - strand, within glnD at 1321.497 kb on + strand, within glnD at 1321.497 kb on + strand, within glnD at 1321.505 kb on - strand, within glnD at 1321.577 kb on - strand, within glnD at 1321.587 kb on - strand, within glnD at 1321.597 kb on + strand, within glnD at 1321.628 kb on + strand, within glnD at 1321.636 kb on - strand, within glnD at 1321.779 kb on + strand, within glnD at 1321.829 kb on - strand, within glnD at 1321.858 kb on - strand, within glnD at 1321.884 kb on + strand, within glnD at 1321.892 kb on - strand, within glnD at 1321.921 kb on + strand, within glnD at 1322.047 kb on + strand, within glnD at 1322.102 kb on + strand, within glnD at 1322.117 kb on + strand, within glnD at 1322.201 kb on + strand, within glnD at 1322.232 kb on + strand, within glnD at 1322.267 kb on + strand, within glnD at 1322.268 kb on - strand, within glnD at 1322.318 kb on - strand, within glnD at 1322.323 kb on + strand, within glnD at 1322.376 kb on - strand, within glnD at 1322.423 kb on - strand, within glnD at 1322.462 kb on - strand, within glnD at 1322.673 kb on + strand, within glnD at 1322.732 kb on + strand, within glnD at 1322.759 kb on - strand, within glnD at 1322.800 kb on - strand at 1322.815 kb on + strand at 1322.815 kb on + strand at 1322.823 kb on - strand at 1322.860 kb on + strand at 1322.860 kb on + strand at 1322.860 kb on + strand at 1322.860 kb on + strand at 1322.868 kb on - strand at 1322.868 kb on - strand at 1322.868 kb on - strand at 1322.868 kb on - strand at 1322.888 kb on - strand at 1322.917 kb on + strand at 1322.925 kb on - strand at 1322.966 kb on + strand at 1322.966 kb on + strand at 1322.973 kb on + strand at 1322.974 kb on - strand at 1322.983 kb on - strand at 1323.015 kb on - strand at 1323.060 kb on + strand at 1323.087 kb on + strand at 1323.098 kb on - strand at 1323.170 kb on - strand at 1323.278 kb on - strand, within DVU1234 at 1323.313 kb on + strand, within DVU1234 at 1323.397 kb on + strand, within DVU1234 at 1323.405 kb on - strand, within DVU1234 at 1323.405 kb on - strand, within DVU1234 at 1323.405 kb on - strand, within DVU1234 at 1323.410 kb on - strand, within DVU1234 at 1323.410 kb on - strand, within DVU1234 at 1323.438 kb on - strand, within DVU1234 at 1323.471 kb on + strand, within DVU1234 at 1323.486 kb on + strand, within DVU1234 at 1323.486 kb on + strand, within DVU1234 at 1323.486 kb on + strand, within DVU1234 at 1323.519 kb on - strand, within DVU1234 at 1323.573 kb on - strand, within DVU1234 at 1323.645 kb on - strand, within DVU1234 at 1323.684 kb on - strand, within DVU1234 at 1323.741 kb on - strand, within DVU1234 at 1323.819 kb on + strand at 1323.847 kb on - strand at 1323.937 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polyethylene glycol 5% remove 1,319,361 + amt DVU1231 0.63 -0.3 1,319,368 + amt DVU1231 0.63 -1.5 1,319,564 - amt DVU1231 0.80 -0.2 1,319,597 + amt DVU1231 0.82 -0.1 1,319,605 - amt DVU1231 0.83 +0.5 1,319,615 + amt DVU1231 0.84 +0.5 1,319,653 + amt DVU1231 0.87 +1.2 1,319,670 + amt DVU1231 0.88 -0.1 1,319,683 + amt DVU1231 0.89 +1.2 1,319,707 + -0.1 1,319,715 - -0.4 1,319,820 - -0.3 1,319,883 - +0.1 1,319,902 + +3.2 1,319,924 - -0.3 1,319,980 + glnB-1 DVU1232 0.14 -0.9 1,320,057 + glnB-1 DVU1232 0.37 -0.1 1,320,105 + glnB-1 DVU1232 0.51 -0.8 1,320,105 + glnB-1 DVU1232 0.51 -0.2 1,320,138 + glnB-1 DVU1232 0.61 -0.7 1,320,146 - glnB-1 DVU1232 0.63 -0.2 1,320,146 - glnB-1 DVU1232 0.63 -0.6 1,320,162 + glnB-1 DVU1232 0.68 -0.4 1,320,168 - glnB-1 DVU1232 0.70 -0.8 1,320,379 + -1.1 1,320,483 - -0.1 1,320,506 - -0.2 1,320,508 + +1.2 1,320,518 + -0.3 1,320,518 + -0.3 1,320,518 + -0.2 1,320,526 - -1.3 1,320,568 + -0.8 1,320,568 + -0.2 1,320,568 + -0.3 1,320,660 - glnD DVU1233 0.12 +0.2 1,320,728 + glnD DVU1233 0.15 -0.5 1,320,743 + glnD DVU1233 0.15 +1.2 1,320,791 - glnD DVU1233 0.17 +0.9 1,320,813 - glnD DVU1233 0.18 +0.0 1,320,814 - glnD DVU1233 0.18 +0.0 1,320,821 - glnD DVU1233 0.18 -0.3 1,320,929 + glnD DVU1233 0.22 +0.3 1,320,938 + glnD DVU1233 0.22 -0.2 1,320,938 + glnD DVU1233 0.22 -0.5 1,321,016 - glnD DVU1233 0.25 +0.2 1,321,060 - glnD DVU1233 0.27 +0.6 1,321,266 + glnD DVU1233 0.35 -0.1 1,321,365 - glnD DVU1233 0.38 +0.0 1,321,497 + glnD DVU1233 0.43 +0.2 1,321,497 + glnD DVU1233 0.43 -0.3 1,321,505 - glnD DVU1233 0.43 -0.0 1,321,577 - glnD DVU1233 0.46 -0.4 1,321,587 - glnD DVU1233 0.46 -2.1 1,321,597 + glnD DVU1233 0.47 -0.0 1,321,628 + glnD DVU1233 0.48 -0.1 1,321,636 - glnD DVU1233 0.48 +2.2 1,321,779 + glnD DVU1233 0.53 +1.2 1,321,829 - glnD DVU1233 0.55 +0.2 1,321,858 - glnD DVU1233 0.56 -2.5 1,321,884 + glnD DVU1233 0.57 -0.2 1,321,892 - glnD DVU1233 0.58 +0.1 1,321,921 + glnD DVU1233 0.59 -0.8 1,322,047 + glnD DVU1233 0.63 -1.8 1,322,102 + glnD DVU1233 0.65 -2.0 1,322,117 + glnD DVU1233 0.66 -0.1 1,322,201 + glnD DVU1233 0.69 -0.2 1,322,232 + glnD DVU1233 0.70 -1.3 1,322,267 + glnD DVU1233 0.71 -0.9 1,322,268 - glnD DVU1233 0.71 -0.0 1,322,318 - glnD DVU1233 0.73 +0.0 1,322,323 + glnD DVU1233 0.73 -1.7 1,322,376 - glnD DVU1233 0.75 -1.0 1,322,423 - glnD DVU1233 0.77 -1.7 1,322,462 - glnD DVU1233 0.79 +0.6 1,322,673 + glnD DVU1233 0.86 -0.3 1,322,732 + glnD DVU1233 0.88 +0.1 1,322,759 - glnD DVU1233 0.89 -1.6 1,322,800 - -1.3 1,322,815 + -0.1 1,322,815 + +0.9 1,322,823 - +0.4 1,322,860 + -0.2 1,322,860 + +0.2 1,322,860 + +0.4 1,322,860 + -0.3 1,322,868 - +0.3 1,322,868 - -1.8 1,322,868 - +0.8 1,322,868 - -1.1 1,322,888 - -0.1 1,322,917 + -0.2 1,322,925 - +0.7 1,322,966 + -0.6 1,322,966 + +0.4 1,322,973 + +2.2 1,322,974 - -0.3 1,322,983 - +0.0 1,323,015 - +0.8 1,323,060 + +0.9 1,323,087 + +0.3 1,323,098 - +0.1 1,323,170 - -0.4 1,323,278 - DVU1234 0.22 +0.1 1,323,313 + DVU1234 0.27 +0.1 1,323,397 + DVU1234 0.38 -1.1 1,323,405 - DVU1234 0.39 -0.3 1,323,405 - DVU1234 0.39 -1.3 1,323,405 - DVU1234 0.39 -0.4 1,323,410 - DVU1234 0.40 -0.4 1,323,410 - DVU1234 0.40 -0.6 1,323,438 - DVU1234 0.44 -0.3 1,323,471 + DVU1234 0.48 +0.5 1,323,486 + DVU1234 0.50 -0.7 1,323,486 + DVU1234 0.50 -1.1 1,323,486 + DVU1234 0.50 -0.6 1,323,519 - DVU1234 0.55 -0.0 1,323,573 - DVU1234 0.62 -0.4 1,323,645 - DVU1234 0.72 +0.1 1,323,684 - DVU1234 0.77 -0.4 1,323,741 - DVU1234 0.85 -0.3 1,323,819 + -0.8 1,323,847 - +0.3 1,323,937 - -0.4
Or see this region's nucleotide sequence