Experiment: MoYLS4 with Polyethylene glycol 1%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tRNA-Gly and tRNA-Cys are separated by 11 nucleotides tRNA-Cys and tRNA-Gly are separated by 7 nucleotides tRNA-Gly and tRNA-Gly are separated by 32 nucleotides tRNA-Gly and DVU2151 are separated by 93 nucleotides DVU2151 and DVU2152 are separated by 9 nucleotides
DVU_tRNA-Gly-1: tRNA-Gly - tRNA with anticodon GCC for Gly (VIMSS-AUTO), at 2,250,385 to 2,250,459
tRNA-Gly
DVU_tRNA-Cys-1: tRNA-Cys - tRNA with anticodon GCA for Cys (VIMSS-AUTO), at 2,250,471 to 2,250,545
tRNA-Cys
DVU_tRNA-Gly-2: tRNA-Gly - tRNA with anticodon GCC for Gly (VIMSS-AUTO), at 2,250,553 to 2,250,627
tRNA-Gly
DVU_tRNA-Gly-3: tRNA-Gly - tRNA with anticodon CCC for Gly (VIMSS-AUTO), at 2,250,660 to 2,250,734
tRNA-Gly
DVU2151: DVU2151 - conserved hypothetical protein (TIGR), at 2,250,828 to 2,251,187
DVU2151
DVU2152: DVU2152 - hypothetical protein (TIGR), at 2,251,197 to 2,252,213
DVU2152
Position (kb)
2250
2251
2252 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2249.863 kb on + strand at 2249.871 kb on - strand at 2249.871 kb on - strand at 2249.898 kb on + strand at 2249.903 kb on + strand at 2250.167 kb on + strand at 2250.188 kb on + strand at 2250.234 kb on - strand at 2250.834 kb on - strand at 2250.843 kb on - strand at 2250.863 kb on + strand at 2250.865 kb on + strand, within DVU2151 at 2250.873 kb on - strand, within DVU2151 at 2250.873 kb on - strand, within DVU2151 at 2250.892 kb on + strand, within DVU2151 at 2251.016 kb on + strand, within DVU2151 at 2251.035 kb on - strand, within DVU2151 at 2251.063 kb on - strand, within DVU2151 at 2251.081 kb on - strand, within DVU2151 at 2251.088 kb on + strand, within DVU2151 at 2251.088 kb on + strand, within DVU2151 at 2251.096 kb on - strand, within DVU2151 at 2251.106 kb on - strand, within DVU2151 at 2251.106 kb on - strand, within DVU2151 at 2251.136 kb on - strand, within DVU2151 at 2251.160 kb on + strand at 2251.192 kb on - strand at 2251.482 kb on + strand, within DVU2152 at 2251.539 kb on + strand, within DVU2152 at 2251.648 kb on - strand, within DVU2152 at 2251.668 kb on - strand, within DVU2152 at 2251.675 kb on + strand, within DVU2152 at 2251.683 kb on - strand, within DVU2152 at 2251.838 kb on + strand, within DVU2152 at 2251.838 kb on + strand, within DVU2152 at 2251.846 kb on - strand, within DVU2152 at 2251.852 kb on + strand, within DVU2152 at 2251.886 kb on - strand, within DVU2152 at 2251.901 kb on + strand, within DVU2152 at 2251.903 kb on + strand, within DVU2152 at 2251.909 kb on - strand, within DVU2152 at 2252.106 kb on + strand, within DVU2152 at 2252.176 kb on + strand at 2252.181 kb on + strand at 2252.181 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Polyethylene glycol 1% remove 2,249,863 + +0.3 2,249,871 - -0.1 2,249,871 - +0.2 2,249,898 + -2.3 2,249,903 + -0.3 2,250,167 + +0.4 2,250,188 + -0.4 2,250,234 - -0.2 2,250,834 - +0.2 2,250,843 - +0.7 2,250,863 + -0.1 2,250,865 + DVU2151 0.10 +0.9 2,250,873 - DVU2151 0.12 -0.1 2,250,873 - DVU2151 0.12 +0.5 2,250,892 + DVU2151 0.18 +0.1 2,251,016 + DVU2151 0.52 +1.7 2,251,035 - DVU2151 0.57 -0.6 2,251,063 - DVU2151 0.65 +0.4 2,251,081 - DVU2151 0.70 -0.5 2,251,088 + DVU2151 0.72 -0.5 2,251,088 + DVU2151 0.72 +2.2 2,251,096 - DVU2151 0.74 +0.0 2,251,106 - DVU2151 0.77 +0.7 2,251,106 - DVU2151 0.77 +0.2 2,251,136 - DVU2151 0.86 +0.0 2,251,160 + -0.1 2,251,192 - +0.6 2,251,482 + DVU2152 0.28 +0.4 2,251,539 + DVU2152 0.34 -0.1 2,251,648 - DVU2152 0.44 +0.0 2,251,668 - DVU2152 0.46 +1.1 2,251,675 + DVU2152 0.47 -0.5 2,251,683 - DVU2152 0.48 -0.2 2,251,838 + DVU2152 0.63 -0.8 2,251,838 + DVU2152 0.63 +1.2 2,251,846 - DVU2152 0.64 -0.0 2,251,852 + DVU2152 0.64 -0.6 2,251,886 - DVU2152 0.68 -0.1 2,251,901 + DVU2152 0.69 -0.3 2,251,903 + DVU2152 0.69 -1.5 2,251,909 - DVU2152 0.70 -0.6 2,252,106 + DVU2152 0.89 -0.8 2,252,176 + -0.6 2,252,181 + -1.3 2,252,181 + +0.1
Or see this region's nucleotide sequence