Strain Fitness in Ralstonia sp. UNC404CL21Col around ABZR87_RS13515

Experiment: Ra1_0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABZR87_RS13505 and ABZR87_RS13510 are separated by 39 nucleotidesABZR87_RS13510 and ABZR87_RS13515 are separated by 3 nucleotidesABZR87_RS13515 and ABZR87_RS13520 are separated by 10 nucleotidesABZR87_RS13520 and ABZR87_RS13525 are separated by 52 nucleotides ABZR87_RS13505: ABZR87_RS13505 - 4-hydroxy-2-oxoheptanedioate aldolase, at 2,863,488 to 2,864,294 _RS13505 ABZR87_RS13510: ABZR87_RS13510 - 2-oxo-hept-4-ene-1,7-dioate hydratase, at 2,864,334 to 2,865,137 _RS13510 ABZR87_RS13515: ABZR87_RS13515 - 5-carboxymethyl-2-hydroxymuconate Delta-isomerase, at 2,865,141 to 2,865,530 _RS13515 ABZR87_RS13520: ABZR87_RS13520 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase, at 2,865,541 to 2,866,410 _RS13520 ABZR87_RS13525: ABZR87_RS13525 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase, at 2,866,463 to 2,867,944 _RS13525 Position (kb) 2865 2866Strain fitness (log2 ratio) -2 -1 0 1 2at 2864.318 kb on - strandat 2864.318 kb on - strandat 2864.336 kb on - strandat 2864.371 kb on + strandat 2864.371 kb on + strandat 2864.387 kb on + strandat 2864.388 kb on - strandat 2864.388 kb on - strandat 2864.388 kb on - strandat 2864.388 kb on - strandat 2864.388 kb on - strandat 2864.388 kb on - strandat 2864.388 kb on - strandat 2864.436 kb on + strand, within ABZR87_RS13510at 2864.437 kb on - strand, within ABZR87_RS13510at 2864.437 kb on - strand, within ABZR87_RS13510at 2864.437 kb on - strand, within ABZR87_RS13510at 2864.756 kb on - strand, within ABZR87_RS13510at 2864.899 kb on + strand, within ABZR87_RS13510at 2864.900 kb on - strand, within ABZR87_RS13510at 2865.181 kb on + strand, within ABZR87_RS13515at 2865.181 kb on + strand, within ABZR87_RS13515at 2865.181 kb on + strand, within ABZR87_RS13515at 2865.182 kb on - strand, within ABZR87_RS13515at 2865.182 kb on - strand, within ABZR87_RS13515at 2865.229 kb on + strand, within ABZR87_RS13515at 2865.509 kb on - strandat 2865.509 kb on - strandat 2865.509 kb on - strandat 2865.644 kb on + strand, within ABZR87_RS13520at 2865.645 kb on - strand, within ABZR87_RS13520at 2865.645 kb on - strand, within ABZR87_RS13520at 2865.645 kb on - strand, within ABZR87_RS13520at 2865.645 kb on - strand, within ABZR87_RS13520at 2865.719 kb on + strand, within ABZR87_RS13520at 2865.767 kb on - strand, within ABZR87_RS13520at 2865.767 kb on - strand, within ABZR87_RS13520at 2865.767 kb on - strand, within ABZR87_RS13520at 2865.767 kb on - strand, within ABZR87_RS13520at 2866.013 kb on + strand, within ABZR87_RS13520at 2866.013 kb on + strand, within ABZR87_RS13520at 2866.013 kb on + strand, within ABZR87_RS13520at 2866.014 kb on - strand, within ABZR87_RS13520at 2866.142 kb on + strand, within ABZR87_RS13520at 2866.142 kb on + strand, within ABZR87_RS13520at 2866.143 kb on - strand, within ABZR87_RS13520at 2866.182 kb on + strand, within ABZR87_RS13520at 2866.183 kb on - strand, within ABZR87_RS13520at 2866.183 kb on - strand, within ABZR87_RS13520at 2866.183 kb on - strand, within ABZR87_RS13520at 2866.184 kb on + strand, within ABZR87_RS13520at 2866.184 kb on + strand, within ABZR87_RS13520at 2866.184 kb on + strand, within ABZR87_RS13520at 2866.361 kb on + strandat 2866.362 kb on - strandat 2866.483 kb on + strandat 2866.483 kb on + strandat 2866.484 kb on - strandat 2866.484 kb on - strandat 2866.484 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ra1_0.1
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2,864,318 - -0.3
2,864,318 - -0.1
2,864,336 - -0.4
2,864,371 + +0.9
2,864,371 + -0.1
2,864,387 + -1.0
2,864,388 - +0.1
2,864,388 - +2.2
2,864,388 - -0.2
2,864,388 - +1.7
2,864,388 - -0.2
2,864,388 - +1.1
2,864,388 - -0.6
2,864,436 + ABZR87_RS13510 0.13 +0.3
2,864,437 - ABZR87_RS13510 0.13 +0.3
2,864,437 - ABZR87_RS13510 0.13 -0.7
2,864,437 - ABZR87_RS13510 0.13 +2.7
2,864,756 - ABZR87_RS13510 0.52 -0.3
2,864,899 + ABZR87_RS13510 0.70 -0.6
2,864,900 - ABZR87_RS13510 0.70 -0.0
2,865,181 + ABZR87_RS13515 0.10 -1.9
2,865,181 + ABZR87_RS13515 0.10 -2.5
2,865,181 + ABZR87_RS13515 0.10 -0.8
2,865,182 - ABZR87_RS13515 0.11 -0.8
2,865,182 - ABZR87_RS13515 0.11 -0.8
2,865,229 + ABZR87_RS13515 0.23 -1.4
2,865,509 - -0.1
2,865,509 - -1.0
2,865,509 - +0.3
2,865,644 + ABZR87_RS13520 0.12 +0.5
2,865,645 - ABZR87_RS13520 0.12 +0.1
2,865,645 - ABZR87_RS13520 0.12 -0.1
2,865,645 - ABZR87_RS13520 0.12 +0.4
2,865,645 - ABZR87_RS13520 0.12 +0.9
2,865,719 + ABZR87_RS13520 0.20 +0.2
2,865,767 - ABZR87_RS13520 0.26 -0.3
2,865,767 - ABZR87_RS13520 0.26 +0.2
2,865,767 - ABZR87_RS13520 0.26 +1.2
2,865,767 - ABZR87_RS13520 0.26 +1.2
2,866,013 + ABZR87_RS13520 0.54 -0.5
2,866,013 + ABZR87_RS13520 0.54 -0.6
2,866,013 + ABZR87_RS13520 0.54 -0.2
2,866,014 - ABZR87_RS13520 0.54 -0.3
2,866,142 + ABZR87_RS13520 0.69 -1.7
2,866,142 + ABZR87_RS13520 0.69 -0.1
2,866,143 - ABZR87_RS13520 0.69 +0.2
2,866,182 + ABZR87_RS13520 0.74 -1.7
2,866,183 - ABZR87_RS13520 0.74 +0.7
2,866,183 - ABZR87_RS13520 0.74 +1.1
2,866,183 - ABZR87_RS13520 0.74 -0.5
2,866,184 + ABZR87_RS13520 0.74 -0.8
2,866,184 + ABZR87_RS13520 0.74 +0.8
2,866,184 + ABZR87_RS13520 0.74 -1.3
2,866,361 + -1.9
2,866,362 - -0.9
2,866,483 + -0.1
2,866,483 + +0.8
2,866,484 - -0.0
2,866,484 - +2.1
2,866,484 - +1.0

Or see this region's nucleotide sequence