Strain Fitness in Ralstonia sp. UNC404CL21Col around ABZR87_RS00135

Experiment: Ra1_0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABZR87_RS00130 and ABZR87_RS00135 are separated by 128 nucleotidesABZR87_RS00135 and ABZR87_RS00140 are separated by 55 nucleotidesABZR87_RS00140 and ABZR87_RS00145 overlap by 4 nucleotidesABZR87_RS00145 and ABZR87_RS00150 are separated by 22 nucleotides ABZR87_RS00130: ABZR87_RS00130 - LysR substrate-binding domain-containing protein, at 25,764 to 26,708 _RS00130 ABZR87_RS00135: ABZR87_RS00135 - dihydroxy-acid dehydratase, at 26,837 to 28,510 _RS00135 ABZR87_RS00140: ABZR87_RS00140 - DUF2486 family protein, at 28,566 to 28,991 _RS00140 ABZR87_RS00145: ABZR87_RS00145 - DNA polymerase III subunit chi, at 28,988 to 29,416 _RS00145 ABZR87_RS00150: ABZR87_RS00150 - leucyl aminopeptidase, at 29,439 to 30,947 _RS00150 Position (kb) 26 27 28 29Strain fitness (log2 ratio) -1 0 1 2 3 4at 25.901 kb on - strand, within ABZR87_RS00130at 25.901 kb on - strand, within ABZR87_RS00130at 25.930 kb on - strand, within ABZR87_RS00130at 25.930 kb on - strand, within ABZR87_RS00130at 26.062 kb on + strand, within ABZR87_RS00130at 26.063 kb on - strand, within ABZR87_RS00130at 26.069 kb on + strand, within ABZR87_RS00130at 26.080 kb on + strand, within ABZR87_RS00130at 26.080 kb on + strand, within ABZR87_RS00130at 26.080 kb on + strand, within ABZR87_RS00130at 26.080 kb on + strand, within ABZR87_RS00130at 26.080 kb on + strand, within ABZR87_RS00130at 26.080 kb on + strand, within ABZR87_RS00130at 26.081 kb on - strand, within ABZR87_RS00130at 26.081 kb on - strand, within ABZR87_RS00130at 26.081 kb on - strand, within ABZR87_RS00130at 26.081 kb on - strand, within ABZR87_RS00130at 26.081 kb on - strand, within ABZR87_RS00130at 26.081 kb on - strand, within ABZR87_RS00130at 26.081 kb on - strand, within ABZR87_RS00130at 26.081 kb on - strand, within ABZR87_RS00130at 26.401 kb on + strand, within ABZR87_RS00130at 26.402 kb on - strand, within ABZR87_RS00130at 26.686 kb on + strandat 26.687 kb on - strandat 26.687 kb on - strandat 26.709 kb on - strandat 26.804 kb on + strandat 26.804 kb on + strandat 26.804 kb on + strandat 26.805 kb on - strandat 26.805 kb on - strandat 26.812 kb on + strandat 26.812 kb on + strandat 26.812 kb on + strandat 26.812 kb on + strandat 26.812 kb on + strandat 26.813 kb on - strandat 26.903 kb on + strandat 26.903 kb on + strandat 26.903 kb on + strandat 26.903 kb on + strandat 26.904 kb on - strandat 26.904 kb on - strandat 26.904 kb on - strandat 26.904 kb on - strandat 26.904 kb on - strandat 26.906 kb on + strandat 26.918 kb on + strandat 26.919 kb on - strandat 27.263 kb on + strand, within ABZR87_RS00135at 27.263 kb on + strand, within ABZR87_RS00135at 27.263 kb on + strand, within ABZR87_RS00135at 27.263 kb on + strand, within ABZR87_RS00135at 27.263 kb on + strand, within ABZR87_RS00135at 27.264 kb on - strand, within ABZR87_RS00135at 27.264 kb on - strand, within ABZR87_RS00135at 27.264 kb on - strand, within ABZR87_RS00135at 27.264 kb on - strand, within ABZR87_RS00135at 27.264 kb on - strand, within ABZR87_RS00135at 27.264 kb on - strand, within ABZR87_RS00135at 27.264 kb on - strand, within ABZR87_RS00135at 27.264 kb on - strand, within ABZR87_RS00135at 27.269 kb on + strand, within ABZR87_RS00135at 27.270 kb on - strand, within ABZR87_RS00135at 27.270 kb on - strand, within ABZR87_RS00135at 27.431 kb on + strand, within ABZR87_RS00135at 27.431 kb on + strand, within ABZR87_RS00135at 27.431 kb on + strand, within ABZR87_RS00135at 27.431 kb on + strand, within ABZR87_RS00135at 27.432 kb on - strand, within ABZR87_RS00135at 27.432 kb on - strand, within ABZR87_RS00135at 27.485 kb on + strand, within ABZR87_RS00135at 27.485 kb on + strand, within ABZR87_RS00135at 27.486 kb on - strand, within ABZR87_RS00135at 27.668 kb on + strand, within ABZR87_RS00135at 27.668 kb on + strand, within ABZR87_RS00135at 27.668 kb on + strand, within ABZR87_RS00135at 27.669 kb on - strand, within ABZR87_RS00135at 27.669 kb on - strand, within ABZR87_RS00135at 27.669 kb on - strand, within ABZR87_RS00135at 27.669 kb on - strand, within ABZR87_RS00135at 27.669 kb on - strand, within ABZR87_RS00135at 27.773 kb on + strand, within ABZR87_RS00135at 27.773 kb on + strand, within ABZR87_RS00135at 27.773 kb on + strand, within ABZR87_RS00135at 27.773 kb on + strand, within ABZR87_RS00135at 27.773 kb on + strand, within ABZR87_RS00135at 27.774 kb on - strand, within ABZR87_RS00135at 27.774 kb on - strand, within ABZR87_RS00135at 27.774 kb on - strand, within ABZR87_RS00135at 27.774 kb on - strand, within ABZR87_RS00135at 27.774 kb on - strand, within ABZR87_RS00135at 27.774 kb on - strand, within ABZR87_RS00135at 27.959 kb on + strand, within ABZR87_RS00135at 27.959 kb on + strand, within ABZR87_RS00135at 27.960 kb on - strand, within ABZR87_RS00135at 28.134 kb on + strand, within ABZR87_RS00135at 28.135 kb on - strand, within ABZR87_RS00135at 28.135 kb on - strand, within ABZR87_RS00135at 28.142 kb on + strand, within ABZR87_RS00135at 28.143 kb on - strand, within ABZR87_RS00135at 28.143 kb on - strand, within ABZR87_RS00135at 28.234 kb on + strand, within ABZR87_RS00135at 28.261 kb on + strand, within ABZR87_RS00135at 28.301 kb on + strand, within ABZR87_RS00135at 28.301 kb on + strand, within ABZR87_RS00135at 28.301 kb on + strand, within ABZR87_RS00135at 28.301 kb on + strand, within ABZR87_RS00135at 28.301 kb on + strand, within ABZR87_RS00135at 28.301 kb on + strand, within ABZR87_RS00135at 28.302 kb on - strand, within ABZR87_RS00135at 28.302 kb on - strand, within ABZR87_RS00135at 28.439 kb on + strandat 28.439 kb on + strandat 28.439 kb on + strandat 28.440 kb on - strandat 28.441 kb on + strandat 28.441 kb on + strandat 28.442 kb on - strandat 28.442 kb on - strandat 28.442 kb on - strandat 28.442 kb on - strandat 28.899 kb on + strand, within ABZR87_RS00140at 28.899 kb on + strand, within ABZR87_RS00140at 28.899 kb on + strand, within ABZR87_RS00140at 28.900 kb on - strand, within ABZR87_RS00140at 28.900 kb on - strand, within ABZR87_RS00140at 28.975 kb on + strandat 29.371 kb on - strand, within ABZR87_RS00145at 29.416 kb on + strandat 29.416 kb on + strandat 29.416 kb on + strandat 29.416 kb on + strandat 29.416 kb on + strandat 29.416 kb on + strandat 29.417 kb on - strandat 29.417 kb on - strandat 29.417 kb on - strandat 29.417 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ra1_0.1
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25,901 - ABZR87_RS00130 0.14 -0.3
25,901 - ABZR87_RS00130 0.14 -1.0
25,930 - ABZR87_RS00130 0.18 +1.7
25,930 - ABZR87_RS00130 0.18 +1.0
26,062 + ABZR87_RS00130 0.32 +0.1
26,063 - ABZR87_RS00130 0.32 +0.1
26,069 + ABZR87_RS00130 0.32 -0.8
26,080 + ABZR87_RS00130 0.33 +0.6
26,080 + ABZR87_RS00130 0.33 -0.9
26,080 + ABZR87_RS00130 0.33 -1.1
26,080 + ABZR87_RS00130 0.33 -0.2
26,080 + ABZR87_RS00130 0.33 +1.3
26,080 + ABZR87_RS00130 0.33 -1.7
26,081 - ABZR87_RS00130 0.34 +0.4
26,081 - ABZR87_RS00130 0.34 +0.8
26,081 - ABZR87_RS00130 0.34 +0.2
26,081 - ABZR87_RS00130 0.34 +0.5
26,081 - ABZR87_RS00130 0.34 +0.5
26,081 - ABZR87_RS00130 0.34 +2.7
26,081 - ABZR87_RS00130 0.34 +0.7
26,081 - ABZR87_RS00130 0.34 +1.9
26,401 + ABZR87_RS00130 0.67 -0.9
26,402 - ABZR87_RS00130 0.68 +0.9
26,686 + -0.9
26,687 - +0.4
26,687 - +0.2
26,709 - +0.8
26,804 + -1.2
26,804 + +0.4
26,804 + +0.0
26,805 - +0.3
26,805 - -0.5
26,812 + +1.0
26,812 + -1.4
26,812 + -1.3
26,812 + +0.8
26,812 + +0.6
26,813 - +1.1
26,903 + -0.8
26,903 + +0.9
26,903 + +0.3
26,903 + -0.3
26,904 - +0.4
26,904 - +0.3
26,904 - +1.0
26,904 - +0.2
26,904 - -0.1
26,906 + -1.2
26,918 + +1.0
26,919 - +1.5
27,263 + ABZR87_RS00135 0.25 -0.3
27,263 + ABZR87_RS00135 0.25 +0.7
27,263 + ABZR87_RS00135 0.25 -0.2
27,263 + ABZR87_RS00135 0.25 -0.2
27,263 + ABZR87_RS00135 0.25 -0.5
27,264 - ABZR87_RS00135 0.26 +0.3
27,264 - ABZR87_RS00135 0.26 +1.2
27,264 - ABZR87_RS00135 0.26 +1.1
27,264 - ABZR87_RS00135 0.26 +0.9
27,264 - ABZR87_RS00135 0.26 +1.1
27,264 - ABZR87_RS00135 0.26 +1.5
27,264 - ABZR87_RS00135 0.26 +2.6
27,264 - ABZR87_RS00135 0.26 +1.3
27,269 + ABZR87_RS00135 0.26 -0.9
27,270 - ABZR87_RS00135 0.26 +0.5
27,270 - ABZR87_RS00135 0.26 -0.1
27,431 + ABZR87_RS00135 0.35 -0.5
27,431 + ABZR87_RS00135 0.35 -0.4
27,431 + ABZR87_RS00135 0.35 +0.9
27,431 + ABZR87_RS00135 0.35 -1.2
27,432 - ABZR87_RS00135 0.36 +1.2
27,432 - ABZR87_RS00135 0.36 +0.7
27,485 + ABZR87_RS00135 0.39 -1.7
27,485 + ABZR87_RS00135 0.39 +0.8
27,486 - ABZR87_RS00135 0.39 +0.2
27,668 + ABZR87_RS00135 0.50 -0.2
27,668 + ABZR87_RS00135 0.50 +0.2
27,668 + ABZR87_RS00135 0.50 +1.5
27,669 - ABZR87_RS00135 0.50 -0.1
27,669 - ABZR87_RS00135 0.50 -0.3
27,669 - ABZR87_RS00135 0.50 +0.4
27,669 - ABZR87_RS00135 0.50 -0.0
27,669 - ABZR87_RS00135 0.50 +0.4
27,773 + ABZR87_RS00135 0.56 -0.4
27,773 + ABZR87_RS00135 0.56 +0.6
27,773 + ABZR87_RS00135 0.56 +1.4
27,773 + ABZR87_RS00135 0.56 +0.4
27,773 + ABZR87_RS00135 0.56 -0.5
27,774 - ABZR87_RS00135 0.56 -0.2
27,774 - ABZR87_RS00135 0.56 +0.6
27,774 - ABZR87_RS00135 0.56 +0.1
27,774 - ABZR87_RS00135 0.56 +1.5
27,774 - ABZR87_RS00135 0.56 +0.7
27,774 - ABZR87_RS00135 0.56 +0.9
27,959 + ABZR87_RS00135 0.67 -0.2
27,959 + ABZR87_RS00135 0.67 +0.9
27,960 - ABZR87_RS00135 0.67 +2.3
28,134 + ABZR87_RS00135 0.77 +0.3
28,135 - ABZR87_RS00135 0.78 +0.1
28,135 - ABZR87_RS00135 0.78 -0.9
28,142 + ABZR87_RS00135 0.78 +0.2
28,143 - ABZR87_RS00135 0.78 +0.9
28,143 - ABZR87_RS00135 0.78 -0.1
28,234 + ABZR87_RS00135 0.83 -0.1
28,261 + ABZR87_RS00135 0.85 +0.7
28,301 + ABZR87_RS00135 0.87 -0.8
28,301 + ABZR87_RS00135 0.87 -1.5
28,301 + ABZR87_RS00135 0.87 -1.5
28,301 + ABZR87_RS00135 0.87 -1.2
28,301 + ABZR87_RS00135 0.87 -0.3
28,301 + ABZR87_RS00135 0.87 -0.2
28,302 - ABZR87_RS00135 0.88 -0.1
28,302 - ABZR87_RS00135 0.88 +0.9
28,439 + +0.6
28,439 + +0.1
28,439 + -0.2
28,440 - +0.2
28,441 + -0.1
28,441 + +0.1
28,442 - +0.7
28,442 - -1.1
28,442 - -0.3
28,442 - +0.5
28,899 + ABZR87_RS00140 0.78 +3.0
28,899 + ABZR87_RS00140 0.78 +1.4
28,899 + ABZR87_RS00140 0.78 +3.1
28,900 - ABZR87_RS00140 0.78 +3.8
28,900 - ABZR87_RS00140 0.78 +3.1
28,975 + +3.4
29,371 - ABZR87_RS00145 0.89 +0.0
29,416 + +1.1
29,416 + +1.7
29,416 + -0.3
29,416 + +1.3
29,416 + +0.6
29,416 + +3.4
29,417 - +1.0
29,417 - -0.9
29,417 - -0.6
29,417 - -0.2

Or see this region's nucleotide sequence