Experiment: Carbon source Phenylacetic 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23725 and RR42_RS23730 are separated by 13 nucleotides RR42_RS23730 and RR42_RS23735 are separated by 15 nucleotides RR42_RS23735 and RR42_RS23740 are separated by 6 nucleotides RR42_RS23740 and RR42_RS23745 are separated by 26 nucleotides
RR42_RS23725: RR42_RS23725 - phenylacetate-CoA oxygenase, at 648,557 to 649,561
_RS23725
RR42_RS23730: RR42_RS23730 - phenylacetate-CoA oxygenase subunit PaaB, at 649,575 to 649,859
_RS23730
RR42_RS23735: RR42_RS23735 - phenylacetyl-CoA 1,2-epoxidase, structural subunit (EC 1.14.13.149) <small>(from data)</small>, at 649,875 to 650,675
_RS23735
RR42_RS23740: RR42_RS23740 - phenylacetate-CoA oxygenase, at 650,682 to 651,248
_RS23740
RR42_RS23745: RR42_RS23745 - phenylacetic acid degradation protein, at 651,275 to 652,363
_RS23745
Position (kb) 649
650
651 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 648.932 kb on + strand, within RR42_RS23725 at 648.932 kb on + strand, within RR42_RS23725 at 648.933 kb on - strand, within RR42_RS23725 at 649.043 kb on + strand, within RR42_RS23725 at 649.043 kb on + strand, within RR42_RS23725 at 649.388 kb on + strand, within RR42_RS23725 at 649.388 kb on + strand, within RR42_RS23725 at 649.614 kb on - strand, within RR42_RS23730 at 649.698 kb on + strand, within RR42_RS23730 at 649.699 kb on - strand, within RR42_RS23730 at 649.699 kb on - strand, within RR42_RS23730 at 649.857 kb on + strand at 649.857 kb on + strand at 649.857 kb on + strand at 649.858 kb on - strand at 649.858 kb on - strand at 649.928 kb on + strand at 649.987 kb on + strand, within RR42_RS23735 at 649.987 kb on + strand, within RR42_RS23735 at 650.038 kb on - strand, within RR42_RS23735 at 650.038 kb on - strand, within RR42_RS23735 at 650.097 kb on + strand, within RR42_RS23735 at 650.097 kb on + strand, within RR42_RS23735 at 650.133 kb on + strand, within RR42_RS23735 at 650.133 kb on + strand, within RR42_RS23735 at 650.133 kb on + strand, within RR42_RS23735 at 650.133 kb on + strand, within RR42_RS23735 at 650.134 kb on - strand, within RR42_RS23735 at 650.134 kb on - strand, within RR42_RS23735 at 650.196 kb on + strand, within RR42_RS23735 at 650.197 kb on - strand, within RR42_RS23735 at 650.197 kb on - strand, within RR42_RS23735 at 650.226 kb on + strand, within RR42_RS23735 at 650.226 kb on + strand, within RR42_RS23735 at 650.410 kb on - strand, within RR42_RS23735 at 650.620 kb on - strand at 650.673 kb on + strand at 650.856 kb on + strand, within RR42_RS23740 at 651.198 kb on + strand at 651.199 kb on - strand at 651.199 kb on - strand at 651.231 kb on + strand at 651.244 kb on - strand at 651.636 kb on - strand, within RR42_RS23745
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Phenylacetic 2.5 mM remove 648,932 + RR42_RS23725 0.37 -3.1 648,932 + RR42_RS23725 0.37 -2.1 648,933 - RR42_RS23725 0.37 -3.1 649,043 + RR42_RS23725 0.48 -2.1 649,043 + RR42_RS23725 0.48 -4.1 649,388 + RR42_RS23725 0.83 -2.1 649,388 + RR42_RS23725 0.83 -3.3 649,614 - RR42_RS23730 0.14 -4.5 649,698 + RR42_RS23730 0.43 -4.2 649,699 - RR42_RS23730 0.44 -3.1 649,699 - RR42_RS23730 0.44 -0.5 649,857 + -4.1 649,857 + -3.4 649,857 + -3.6 649,858 - -3.0 649,858 - -3.9 649,928 + -1.2 649,987 + RR42_RS23735 0.14 -2.2 649,987 + RR42_RS23735 0.14 -4.8 650,038 - RR42_RS23735 0.20 -2.8 650,038 - RR42_RS23735 0.20 -1.5 650,097 + RR42_RS23735 0.28 -1.8 650,097 + RR42_RS23735 0.28 +0.1 650,133 + RR42_RS23735 0.32 +0.5 650,133 + RR42_RS23735 0.32 -3.5 650,133 + RR42_RS23735 0.32 -3.6 650,133 + RR42_RS23735 0.32 -2.2 650,134 - RR42_RS23735 0.32 -2.3 650,134 - RR42_RS23735 0.32 -1.4 650,196 + RR42_RS23735 0.40 -3.2 650,197 - RR42_RS23735 0.40 -4.5 650,197 - RR42_RS23735 0.40 -3.7 650,226 + RR42_RS23735 0.44 -4.4 650,226 + RR42_RS23735 0.44 -2.6 650,410 - RR42_RS23735 0.67 -2.9 650,620 - -1.8 650,673 + -1.2 650,856 + RR42_RS23740 0.31 -1.1 651,198 + -0.8 651,199 - -3.5 651,199 - -3.2 651,231 + -2.8 651,244 - -1.7 651,636 - RR42_RS23745 0.33 -0.1
Or see this region's nucleotide sequence