Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS19700

Experiment: Carbon source Phenylacetic 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS19690 and RR42_RS19695 are separated by 172 nucleotidesRR42_RS19695 and RR42_RS19700 are separated by 120 nucleotidesRR42_RS19700 and RR42_RS19705 are separated by 25 nucleotides RR42_RS19690: RR42_RS19690 - Tat pathway signal protein, at 4,253,737 to 4,254,192 _RS19690 RR42_RS19695: RR42_RS19695 - cytochrome C protein, at 4,254,365 to 4,254,694 _RS19695 RR42_RS19700: RR42_RS19700 - cytochrome C, at 4,254,815 to 4,255,828 _RS19700 RR42_RS19705: RR42_RS19705 - sulfite dehydrogenase, at 4,255,854 to 4,257,218 _RS19705 Position (kb) 4254 4255 4256Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4253.816 kb on + strand, within RR42_RS19690at 4253.817 kb on - strand, within RR42_RS19690at 4254.169 kb on + strandat 4254.169 kb on + strandat 4254.170 kb on - strandat 4254.366 kb on + strandat 4254.366 kb on + strandat 4254.366 kb on + strandat 4254.366 kb on + strandat 4254.366 kb on + strandat 4254.366 kb on + strandat 4254.366 kb on + strandat 4254.367 kb on - strandat 4254.367 kb on - strandat 4254.367 kb on - strandat 4254.367 kb on - strandat 4254.367 kb on - strandat 4254.367 kb on - strandat 4254.526 kb on - strand, within RR42_RS19695at 4254.526 kb on - strand, within RR42_RS19695at 4254.526 kb on - strand, within RR42_RS19695at 4254.546 kb on + strand, within RR42_RS19695at 4254.697 kb on + strandat 4254.698 kb on + strandat 4254.698 kb on + strandat 4254.698 kb on + strandat 4254.698 kb on + strandat 4254.699 kb on - strandat 4254.699 kb on - strandat 4254.699 kb on - strandat 4254.753 kb on + strandat 4254.964 kb on - strand, within RR42_RS19700at 4255.036 kb on - strand, within RR42_RS19700at 4255.389 kb on + strand, within RR42_RS19700at 4255.390 kb on - strand, within RR42_RS19700at 4255.404 kb on + strand, within RR42_RS19700at 4255.461 kb on + strand, within RR42_RS19700at 4255.461 kb on + strand, within RR42_RS19700at 4255.462 kb on - strand, within RR42_RS19700at 4255.806 kb on - strandat 4255.855 kb on + strandat 4255.855 kb on + strandat 4255.855 kb on + strandat 4255.856 kb on - strandat 4255.925 kb on - strandat 4255.925 kb on - strandat 4256.176 kb on + strand, within RR42_RS19705at 4256.176 kb on + strand, within RR42_RS19705at 4256.177 kb on - strand, within RR42_RS19705at 4256.177 kb on - strand, within RR42_RS19705at 4256.223 kb on - strand, within RR42_RS19705at 4256.223 kb on - strand, within RR42_RS19705at 4256.287 kb on + strand, within RR42_RS19705at 4256.288 kb on - strand, within RR42_RS19705at 4256.519 kb on - strand, within RR42_RS19705at 4256.611 kb on + strand, within RR42_RS19705at 4256.612 kb on - strand, within RR42_RS19705at 4256.654 kb on + strand, within RR42_RS19705at 4256.654 kb on + strand, within RR42_RS19705at 4256.655 kb on - strand, within RR42_RS19705at 4256.723 kb on - strand, within RR42_RS19705

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbon source Phenylacetic 2.5 mM
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4,253,816 + RR42_RS19690 0.17 -0.1
4,253,817 - RR42_RS19690 0.18 +0.2
4,254,169 + +0.0
4,254,169 + -0.3
4,254,170 - -0.5
4,254,366 + +0.9
4,254,366 + -0.6
4,254,366 + -0.1
4,254,366 + -0.0
4,254,366 + -0.3
4,254,366 + +0.8
4,254,366 + -0.5
4,254,367 - +1.2
4,254,367 - -0.1
4,254,367 - +1.0
4,254,367 - +0.3
4,254,367 - +1.0
4,254,367 - +0.6
4,254,526 - RR42_RS19695 0.49 -1.5
4,254,526 - RR42_RS19695 0.49 -1.1
4,254,526 - RR42_RS19695 0.49 -0.7
4,254,546 + RR42_RS19695 0.55 -0.1
4,254,697 + +1.0
4,254,698 + -0.3
4,254,698 + -0.8
4,254,698 + +1.4
4,254,698 + +0.1
4,254,699 - +0.2
4,254,699 - +0.4
4,254,699 - -0.2
4,254,753 + -0.4
4,254,964 - RR42_RS19700 0.15 +0.3
4,255,036 - RR42_RS19700 0.22 -1.2
4,255,389 + RR42_RS19700 0.57 +0.4
4,255,390 - RR42_RS19700 0.57 -0.4
4,255,404 + RR42_RS19700 0.58 +0.0
4,255,461 + RR42_RS19700 0.64 -1.9
4,255,461 + RR42_RS19700 0.64 +1.3
4,255,462 - RR42_RS19700 0.64 +0.4
4,255,806 - +0.1
4,255,855 + -0.1
4,255,855 + -1.8
4,255,855 + -1.2
4,255,856 - +0.1
4,255,925 - -0.5
4,255,925 - +0.2
4,256,176 + RR42_RS19705 0.24 +0.1
4,256,176 + RR42_RS19705 0.24 +1.3
4,256,177 - RR42_RS19705 0.24 +2.5
4,256,177 - RR42_RS19705 0.24 +1.6
4,256,223 - RR42_RS19705 0.27 +0.9
4,256,223 - RR42_RS19705 0.27 -0.5
4,256,287 + RR42_RS19705 0.32 -0.8
4,256,288 - RR42_RS19705 0.32 -1.1
4,256,519 - RR42_RS19705 0.49 -0.1
4,256,611 + RR42_RS19705 0.55 -3.2
4,256,612 - RR42_RS19705 0.56 +1.9
4,256,654 + RR42_RS19705 0.59 +0.8
4,256,654 + RR42_RS19705 0.59 -0.1
4,256,655 - RR42_RS19705 0.59 -1.7
4,256,723 - RR42_RS19705 0.64 +0.8

Or see this region's nucleotide sequence