Experiment: Recovery broth with Thioridazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13585 and BBR_RS13590 are separated by 31 nucleotides BBR_RS13590 and BBR_RS13595 are separated by 379 nucleotides BBR_RS13595 and BBR_RS13600 are separated by 238 nucleotides
BBR_RS13585: BBR_RS13585 - transporter, at 818,642 to 819,712
_RS13585
BBR_RS13590: BBR_RS13590 - hypothetical protein, at 819,744 to 819,993
_RS13590
BBR_RS13595: BBR_RS13595 - glycosyl transferase, at 820,373 to 822,640
_RS13595
BBR_RS13600: BBR_RS13600 - carbohydrate-binding protein, at 822,879 to 824,192
_RS13600
Position (kb) 820
821
822
823 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 819.415 kb on + strand, within BBR_RS13585 at 819.425 kb on - strand, within BBR_RS13585 at 819.425 kb on - strand, within BBR_RS13585 at 819.447 kb on + strand, within BBR_RS13585 at 819.447 kb on + strand, within BBR_RS13585 at 819.506 kb on + strand, within BBR_RS13585 at 819.506 kb on + strand, within BBR_RS13585 at 819.519 kb on - strand, within BBR_RS13585 at 819.583 kb on + strand, within BBR_RS13585 at 819.622 kb on + strand at 819.701 kb on + strand at 819.719 kb on + strand at 819.764 kb on + strand at 819.772 kb on - strand, within BBR_RS13590 at 819.782 kb on - strand, within BBR_RS13590 at 819.803 kb on + strand, within BBR_RS13590 at 819.810 kb on - strand, within BBR_RS13590 at 819.811 kb on - strand, within BBR_RS13590 at 819.811 kb on - strand, within BBR_RS13590 at 819.836 kb on - strand, within BBR_RS13590 at 819.848 kb on - strand, within BBR_RS13590 at 819.897 kb on + strand, within BBR_RS13590 at 819.908 kb on + strand, within BBR_RS13590 at 819.915 kb on - strand, within BBR_RS13590 at 819.927 kb on - strand, within BBR_RS13590 at 819.988 kb on + strand at 819.990 kb on + strand at 819.996 kb on - strand at 820.003 kb on + strand at 820.054 kb on - strand at 820.054 kb on - strand at 820.134 kb on + strand at 820.177 kb on + strand at 820.258 kb on + strand at 820.301 kb on + strand at 820.301 kb on + strand at 820.309 kb on - strand at 820.310 kb on + strand at 820.366 kb on - strand at 820.442 kb on + strand at 820.580 kb on + strand at 820.665 kb on + strand, within BBR_RS13595 at 820.665 kb on + strand, within BBR_RS13595 at 820.665 kb on + strand, within BBR_RS13595 at 820.747 kb on + strand, within BBR_RS13595 at 820.819 kb on + strand, within BBR_RS13595 at 820.837 kb on - strand, within BBR_RS13595 at 820.851 kb on + strand, within BBR_RS13595 at 820.894 kb on + strand, within BBR_RS13595 at 820.950 kb on + strand, within BBR_RS13595 at 820.987 kb on - strand, within BBR_RS13595 at 821.033 kb on + strand, within BBR_RS13595 at 821.041 kb on - strand, within BBR_RS13595 at 821.170 kb on + strand, within BBR_RS13595 at 821.170 kb on + strand, within BBR_RS13595 at 821.259 kb on + strand, within BBR_RS13595 at 821.340 kb on + strand, within BBR_RS13595 at 821.445 kb on + strand, within BBR_RS13595 at 821.467 kb on + strand, within BBR_RS13595 at 821.475 kb on - strand, within BBR_RS13595 at 821.475 kb on - strand, within BBR_RS13595 at 821.488 kb on - strand, within BBR_RS13595 at 821.499 kb on - strand, within BBR_RS13595 at 821.602 kb on + strand, within BBR_RS13595 at 821.644 kb on - strand, within BBR_RS13595 at 821.677 kb on - strand, within BBR_RS13595 at 821.691 kb on + strand, within BBR_RS13595 at 821.715 kb on - strand, within BBR_RS13595 at 821.741 kb on + strand, within BBR_RS13595 at 821.756 kb on + strand, within BBR_RS13595 at 821.766 kb on - strand, within BBR_RS13595 at 821.813 kb on + strand, within BBR_RS13595 at 821.818 kb on + strand, within BBR_RS13595 at 821.901 kb on + strand, within BBR_RS13595 at 821.909 kb on - strand, within BBR_RS13595 at 821.922 kb on - strand, within BBR_RS13595 at 821.944 kb on + strand, within BBR_RS13595 at 821.944 kb on + strand, within BBR_RS13595 at 821.944 kb on + strand, within BBR_RS13595 at 821.945 kb on - strand, within BBR_RS13595 at 821.945 kb on - strand, within BBR_RS13595 at 821.952 kb on - strand, within BBR_RS13595 at 821.954 kb on + strand, within BBR_RS13595 at 821.954 kb on - strand, within BBR_RS13595 at 821.962 kb on - strand, within BBR_RS13595 at 821.962 kb on - strand, within BBR_RS13595 at 821.962 kb on - strand, within BBR_RS13595 at 821.962 kb on - strand, within BBR_RS13595 at 821.979 kb on - strand, within BBR_RS13595 at 821.981 kb on + strand, within BBR_RS13595 at 821.991 kb on - strand, within BBR_RS13595 at 822.080 kb on - strand, within BBR_RS13595 at 822.081 kb on - strand, within BBR_RS13595 at 822.085 kb on + strand, within BBR_RS13595 at 822.140 kb on + strand, within BBR_RS13595 at 822.183 kb on - strand, within BBR_RS13595 at 822.201 kb on + strand, within BBR_RS13595 at 822.211 kb on + strand, within BBR_RS13595 at 822.244 kb on - strand, within BBR_RS13595 at 822.249 kb on + strand, within BBR_RS13595 at 822.322 kb on - strand, within BBR_RS13595 at 822.328 kb on + strand, within BBR_RS13595 at 822.347 kb on - strand, within BBR_RS13595 at 822.424 kb on - strand at 822.448 kb on - strand at 822.458 kb on + strand at 822.469 kb on + strand at 822.503 kb on + strand at 822.508 kb on + strand at 822.536 kb on - strand at 822.583 kb on - strand at 822.583 kb on - strand at 822.583 kb on - strand at 822.583 kb on - strand at 822.714 kb on - strand at 822.716 kb on + strand at 822.716 kb on + strand at 822.716 kb on + strand at 822.716 kb on + strand at 822.716 kb on + strand at 822.716 kb on + strand at 822.716 kb on + strand at 822.724 kb on - strand at 822.724 kb on - strand at 822.724 kb on - strand at 822.724 kb on - strand at 822.737 kb on + strand at 822.745 kb on - strand at 822.761 kb on - strand at 822.795 kb on - strand at 822.814 kb on + strand at 822.822 kb on - strand at 822.822 kb on - strand at 822.833 kb on + strand at 822.896 kb on - strand at 822.931 kb on + strand at 822.971 kb on + strand at 823.041 kb on - strand, within BBR_RS13600 at 823.062 kb on - strand, within BBR_RS13600 at 823.079 kb on + strand, within BBR_RS13600 at 823.079 kb on + strand, within BBR_RS13600 at 823.203 kb on + strand, within BBR_RS13600 at 823.248 kb on + strand, within BBR_RS13600 at 823.266 kb on - strand, within BBR_RS13600 at 823.356 kb on - strand, within BBR_RS13600 at 823.412 kb on - strand, within BBR_RS13600 at 823.560 kb on + strand, within BBR_RS13600 at 823.568 kb on - strand, within BBR_RS13600
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Thioridazine 0.1 mM remove 819,415 + BBR_RS13585 0.72 +1.6 819,425 - BBR_RS13585 0.73 -1.4 819,425 - BBR_RS13585 0.73 -1.2 819,447 + BBR_RS13585 0.75 +1.9 819,447 + BBR_RS13585 0.75 +0.0 819,506 + BBR_RS13585 0.81 -0.3 819,506 + BBR_RS13585 0.81 +0.8 819,519 - BBR_RS13585 0.82 +0.8 819,583 + BBR_RS13585 0.88 +1.5 819,622 + -0.6 819,701 + +3.0 819,719 + +3.1 819,764 + +1.3 819,772 - BBR_RS13590 0.11 -2.3 819,782 - BBR_RS13590 0.15 +0.8 819,803 + BBR_RS13590 0.24 +0.3 819,810 - BBR_RS13590 0.26 -0.8 819,811 - BBR_RS13590 0.27 +0.8 819,811 - BBR_RS13590 0.27 +1.0 819,836 - BBR_RS13590 0.37 -1.8 819,848 - BBR_RS13590 0.42 -1.4 819,897 + BBR_RS13590 0.61 +0.4 819,908 + BBR_RS13590 0.66 -0.4 819,915 - BBR_RS13590 0.68 +0.1 819,927 - BBR_RS13590 0.73 -1.9 819,988 + -1.3 819,990 + -1.6 819,996 - +0.7 820,003 + +1.9 820,054 - -0.2 820,054 - +0.9 820,134 + +0.7 820,177 + +0.8 820,258 + +1.7 820,301 + +1.5 820,301 + -0.6 820,309 - -0.8 820,310 + -0.4 820,366 - -0.6 820,442 + +1.6 820,580 + +0.4 820,665 + BBR_RS13595 0.13 +0.8 820,665 + BBR_RS13595 0.13 +1.1 820,665 + BBR_RS13595 0.13 -2.1 820,747 + BBR_RS13595 0.16 -0.2 820,819 + BBR_RS13595 0.20 +0.3 820,837 - BBR_RS13595 0.20 +1.8 820,851 + BBR_RS13595 0.21 +1.0 820,894 + BBR_RS13595 0.23 +2.3 820,950 + BBR_RS13595 0.25 +2.5 820,987 - BBR_RS13595 0.27 +0.1 821,033 + BBR_RS13595 0.29 +1.4 821,041 - BBR_RS13595 0.29 +0.7 821,170 + BBR_RS13595 0.35 +1.4 821,170 + BBR_RS13595 0.35 -1.2 821,259 + BBR_RS13595 0.39 -1.6 821,340 + BBR_RS13595 0.43 +1.4 821,445 + BBR_RS13595 0.47 -1.1 821,467 + BBR_RS13595 0.48 -0.5 821,475 - BBR_RS13595 0.49 -0.0 821,475 - BBR_RS13595 0.49 +2.6 821,488 - BBR_RS13595 0.49 +0.4 821,499 - BBR_RS13595 0.50 -0.8 821,602 + BBR_RS13595 0.54 +1.2 821,644 - BBR_RS13595 0.56 -1.2 821,677 - BBR_RS13595 0.57 -0.7 821,691 + BBR_RS13595 0.58 -1.4 821,715 - BBR_RS13595 0.59 +2.6 821,741 + BBR_RS13595 0.60 +1.9 821,756 + BBR_RS13595 0.61 +1.1 821,766 - BBR_RS13595 0.61 -1.3 821,813 + BBR_RS13595 0.63 +0.5 821,818 + BBR_RS13595 0.64 +0.4 821,901 + BBR_RS13595 0.67 +0.6 821,909 - BBR_RS13595 0.68 +2.4 821,922 - BBR_RS13595 0.68 -1.4 821,944 + BBR_RS13595 0.69 -0.8 821,944 + BBR_RS13595 0.69 +1.4 821,944 + BBR_RS13595 0.69 +2.6 821,945 - BBR_RS13595 0.69 -0.3 821,945 - BBR_RS13595 0.69 -1.1 821,952 - BBR_RS13595 0.70 -1.3 821,954 + BBR_RS13595 0.70 -0.4 821,954 - BBR_RS13595 0.70 -0.3 821,962 - BBR_RS13595 0.70 +2.2 821,962 - BBR_RS13595 0.70 -1.4 821,962 - BBR_RS13595 0.70 +0.3 821,962 - BBR_RS13595 0.70 -2.3 821,979 - BBR_RS13595 0.71 -2.4 821,981 + BBR_RS13595 0.71 +1.0 821,991 - BBR_RS13595 0.71 +0.2 822,080 - BBR_RS13595 0.75 -1.1 822,081 - BBR_RS13595 0.75 -2.2 822,085 + BBR_RS13595 0.75 +1.5 822,140 + BBR_RS13595 0.78 -1.0 822,183 - BBR_RS13595 0.80 +1.2 822,201 + BBR_RS13595 0.81 +0.2 822,211 + BBR_RS13595 0.81 +2.6 822,244 - BBR_RS13595 0.82 -2.4 822,249 + BBR_RS13595 0.83 -0.6 822,322 - BBR_RS13595 0.86 +0.6 822,328 + BBR_RS13595 0.86 +0.9 822,347 - BBR_RS13595 0.87 +1.1 822,424 - +0.8 822,448 - -0.5 822,458 + -0.4 822,469 + -0.6 822,503 + +0.1 822,508 + -1.0 822,536 - -0.9 822,583 - -2.7 822,583 - +1.8 822,583 - -1.5 822,583 - -1.2 822,714 - -1.0 822,716 + +3.2 822,716 + +0.9 822,716 + +2.3 822,716 + -0.1 822,716 + -0.9 822,716 + -0.3 822,716 + +0.6 822,724 - -1.6 822,724 - +0.3 822,724 - -0.9 822,724 - -0.4 822,737 + -3.8 822,745 - -1.9 822,761 - -1.8 822,795 - +0.5 822,814 + +1.5 822,822 - +1.3 822,822 - -1.4 822,833 + -1.8 822,896 - +0.8 822,931 + +0.1 822,971 + -0.4 823,041 - BBR_RS13600 0.12 -1.6 823,062 - BBR_RS13600 0.14 -0.4 823,079 + BBR_RS13600 0.15 +0.4 823,079 + BBR_RS13600 0.15 +1.3 823,203 + BBR_RS13600 0.25 -2.0 823,248 + BBR_RS13600 0.28 +2.1 823,266 - BBR_RS13600 0.29 +1.5 823,356 - BBR_RS13600 0.36 -1.0 823,412 - BBR_RS13600 0.41 +0.3 823,560 + BBR_RS13600 0.52 +0.3 823,568 - BBR_RS13600 0.52 +0.0
Or see this region's nucleotide sequence