Experiment: Recovery broth with Thioridazine 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12180 and BBR_RS12185 are separated by 269 nucleotides BBR_RS12185 and BBR_RS12190 are separated by 289 nucleotides BBR_RS12190 and BBR_RS12195 are separated by 164 nucleotides
BBR_RS12180: BBR_RS12180 - DUF2974 domain-containing protein, at 481,393 to 482,730
_RS12180
BBR_RS12185: BBR_RS12185 - methylated-DNA--[protein]-cysteine S-methyltransferase, at 483,000 to 483,554
_RS12185
BBR_RS12190: BBR_RS12190 - holo-ACP synthase, at 483,844 to 484,293
_RS12190
BBR_RS12195: BBR_RS12195 - alpha-amylase, at 484,458 to 485,000
_RS12195
Position (kb) 482
483
484 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 482.010 kb on - strand, within BBR_RS12180 at 482.010 kb on - strand, within BBR_RS12180 at 482.054 kb on + strand, within BBR_RS12180 at 482.062 kb on - strand, within BBR_RS12180 at 482.076 kb on + strand, within BBR_RS12180 at 482.112 kb on + strand, within BBR_RS12180 at 482.120 kb on - strand, within BBR_RS12180 at 482.120 kb on - strand, within BBR_RS12180 at 482.120 kb on - strand, within BBR_RS12180 at 482.139 kb on + strand, within BBR_RS12180 at 482.139 kb on + strand, within BBR_RS12180 at 482.139 kb on + strand, within BBR_RS12180 at 482.139 kb on + strand, within BBR_RS12180 at 482.147 kb on - strand, within BBR_RS12180 at 482.147 kb on - strand, within BBR_RS12180 at 482.147 kb on - strand, within BBR_RS12180 at 482.147 kb on - strand, within BBR_RS12180 at 482.147 kb on - strand, within BBR_RS12180 at 482.169 kb on + strand, within BBR_RS12180 at 482.170 kb on - strand, within BBR_RS12180 at 482.202 kb on + strand, within BBR_RS12180 at 482.202 kb on + strand, within BBR_RS12180 at 482.214 kb on + strand, within BBR_RS12180 at 482.232 kb on + strand, within BBR_RS12180 at 482.237 kb on + strand, within BBR_RS12180 at 482.240 kb on - strand, within BBR_RS12180 at 482.246 kb on - strand, within BBR_RS12180 at 482.255 kb on - strand, within BBR_RS12180 at 482.303 kb on + strand, within BBR_RS12180 at 482.305 kb on + strand, within BBR_RS12180 at 482.339 kb on - strand, within BBR_RS12180 at 482.339 kb on - strand, within BBR_RS12180 at 482.339 kb on - strand, within BBR_RS12180 at 482.339 kb on - strand, within BBR_RS12180 at 482.339 kb on - strand, within BBR_RS12180 at 482.347 kb on + strand, within BBR_RS12180 at 482.347 kb on + strand, within BBR_RS12180 at 482.376 kb on + strand, within BBR_RS12180 at 482.376 kb on + strand, within BBR_RS12180 at 482.376 kb on + strand, within BBR_RS12180 at 482.376 kb on + strand, within BBR_RS12180 at 482.376 kb on + strand, within BBR_RS12180 at 482.376 kb on + strand, within BBR_RS12180 at 482.376 kb on + strand, within BBR_RS12180 at 482.376 kb on + strand, within BBR_RS12180 at 482.376 kb on + strand, within BBR_RS12180 at 482.376 kb on + strand, within BBR_RS12180 at 482.378 kb on + strand, within BBR_RS12180 at 482.380 kb on - strand, within BBR_RS12180 at 482.384 kb on - strand, within BBR_RS12180 at 482.384 kb on - strand, within BBR_RS12180 at 482.384 kb on - strand, within BBR_RS12180 at 482.384 kb on - strand, within BBR_RS12180 at 482.384 kb on - strand, within BBR_RS12180 at 482.384 kb on - strand, within BBR_RS12180 at 482.384 kb on - strand, within BBR_RS12180 at 482.384 kb on - strand, within BBR_RS12180 at 482.384 kb on - strand, within BBR_RS12180 at 482.386 kb on - strand, within BBR_RS12180 at 482.388 kb on + strand, within BBR_RS12180 at 482.388 kb on + strand, within BBR_RS12180 at 482.390 kb on - strand, within BBR_RS12180 at 482.391 kb on - strand, within BBR_RS12180 at 482.409 kb on + strand, within BBR_RS12180 at 482.410 kb on + strand, within BBR_RS12180 at 482.410 kb on + strand, within BBR_RS12180 at 482.415 kb on - strand, within BBR_RS12180 at 482.433 kb on - strand, within BBR_RS12180 at 482.478 kb on + strand, within BBR_RS12180 at 482.478 kb on + strand, within BBR_RS12180 at 482.478 kb on - strand, within BBR_RS12180 at 482.486 kb on - strand, within BBR_RS12180 at 482.496 kb on - strand, within BBR_RS12180 at 482.524 kb on - strand, within BBR_RS12180 at 482.524 kb on - strand, within BBR_RS12180 at 482.538 kb on - strand, within BBR_RS12180 at 482.544 kb on + strand, within BBR_RS12180 at 482.557 kb on - strand, within BBR_RS12180 at 482.589 kb on - strand, within BBR_RS12180 at 482.604 kb on + strand at 482.604 kb on + strand at 482.604 kb on + strand at 482.604 kb on + strand at 482.604 kb on + strand at 482.609 kb on + strand at 482.612 kb on - strand at 482.622 kb on - strand at 482.622 kb on - strand at 482.622 kb on - strand at 482.706 kb on + strand at 482.714 kb on - strand at 482.726 kb on + strand at 482.726 kb on + strand at 482.750 kb on + strand at 482.771 kb on - strand at 482.789 kb on - strand at 482.798 kb on + strand at 482.820 kb on + strand at 482.829 kb on + strand at 482.857 kb on + strand at 482.871 kb on - strand at 482.871 kb on - strand at 482.884 kb on - strand at 482.928 kb on + strand at 482.928 kb on + strand at 482.928 kb on + strand at 482.928 kb on + strand at 482.928 kb on + strand at 482.933 kb on + strand at 482.936 kb on - strand at 482.936 kb on - strand at 482.936 kb on - strand at 482.936 kb on - strand at 482.936 kb on - strand at 482.936 kb on - strand at 482.936 kb on - strand at 482.938 kb on - strand at 482.943 kb on - strand at 482.943 kb on - strand at 482.997 kb on + strand at 482.997 kb on + strand at 483.005 kb on - strand at 483.016 kb on - strand at 483.039 kb on + strand at 483.039 kb on + strand at 483.046 kb on - strand at 483.047 kb on + strand at 483.051 kb on + strand at 483.052 kb on + strand at 483.054 kb on - strand at 483.060 kb on - strand, within BBR_RS12185 at 483.075 kb on - strand, within BBR_RS12185 at 483.107 kb on + strand, within BBR_RS12185 at 483.112 kb on + strand, within BBR_RS12185 at 483.141 kb on - strand, within BBR_RS12185 at 483.141 kb on - strand, within BBR_RS12185 at 483.147 kb on + strand, within BBR_RS12185 at 483.164 kb on + strand, within BBR_RS12185 at 483.164 kb on - strand, within BBR_RS12185 at 483.169 kb on + strand, within BBR_RS12185 at 483.187 kb on + strand, within BBR_RS12185 at 483.216 kb on - strand, within BBR_RS12185 at 483.226 kb on - strand, within BBR_RS12185 at 483.233 kb on + strand, within BBR_RS12185 at 483.233 kb on + strand, within BBR_RS12185 at 483.233 kb on + strand, within BBR_RS12185 at 483.233 kb on + strand, within BBR_RS12185 at 483.245 kb on + strand, within BBR_RS12185 at 483.293 kb on + strand, within BBR_RS12185 at 483.329 kb on + strand, within BBR_RS12185 at 483.335 kb on + strand, within BBR_RS12185 at 483.336 kb on + strand, within BBR_RS12185 at 483.343 kb on - strand, within BBR_RS12185 at 483.355 kb on + strand, within BBR_RS12185 at 483.363 kb on - strand, within BBR_RS12185 at 483.368 kb on - strand, within BBR_RS12185 at 483.370 kb on - strand, within BBR_RS12185 at 483.380 kb on - strand, within BBR_RS12185 at 483.384 kb on + strand, within BBR_RS12185 at 483.386 kb on + strand, within BBR_RS12185 at 483.398 kb on + strand, within BBR_RS12185 at 483.409 kb on + strand, within BBR_RS12185 at 483.425 kb on - strand, within BBR_RS12185 at 483.450 kb on - strand, within BBR_RS12185 at 483.460 kb on + strand, within BBR_RS12185 at 483.463 kb on - strand, within BBR_RS12185 at 483.463 kb on - strand, within BBR_RS12185 at 483.485 kb on - strand, within BBR_RS12185 at 483.486 kb on + strand, within BBR_RS12185 at 483.500 kb on + strand at 483.551 kb on - strand at 483.621 kb on + strand at 483.639 kb on + strand at 483.639 kb on + strand at 483.647 kb on - strand at 483.654 kb on - strand at 483.687 kb on + strand at 483.687 kb on + strand at 483.687 kb on + strand at 483.687 kb on + strand at 483.687 kb on + strand at 483.695 kb on - strand at 483.718 kb on - strand at 483.758 kb on + strand at 483.763 kb on + strand at 483.805 kb on + strand at 483.813 kb on - strand at 483.825 kb on + strand at 483.833 kb on - strand at 484.324 kb on + strand at 484.334 kb on - strand at 484.353 kb on + strand at 484.395 kb on + strand at 484.396 kb on + strand at 484.403 kb on - strand at 484.420 kb on + strand at 484.453 kb on + strand at 484.459 kb on - strand at 484.466 kb on + strand at 484.492 kb on - strand at 484.511 kb on + strand at 484.511 kb on + strand at 484.518 kb on + strand, within BBR_RS12195 at 484.518 kb on + strand, within BBR_RS12195 at 484.518 kb on + strand, within BBR_RS12195 at 484.544 kb on + strand, within BBR_RS12195 at 484.550 kb on - strand, within BBR_RS12195 at 484.551 kb on + strand, within BBR_RS12195 at 484.552 kb on - strand, within BBR_RS12195 at 484.552 kb on - strand, within BBR_RS12195 at 484.552 kb on - strand, within BBR_RS12195 at 484.554 kb on - strand, within BBR_RS12195
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Thioridazine 0.1 mM remove 482,010 - BBR_RS12180 0.46 -1.3 482,010 - BBR_RS12180 0.46 -1.7 482,054 + BBR_RS12180 0.49 -1.3 482,062 - BBR_RS12180 0.50 +0.3 482,076 + BBR_RS12180 0.51 +0.2 482,112 + BBR_RS12180 0.54 -0.7 482,120 - BBR_RS12180 0.54 -2.5 482,120 - BBR_RS12180 0.54 -0.2 482,120 - BBR_RS12180 0.54 +0.1 482,139 + BBR_RS12180 0.56 -1.4 482,139 + BBR_RS12180 0.56 -1.5 482,139 + BBR_RS12180 0.56 -1.3 482,139 + BBR_RS12180 0.56 -2.0 482,147 - BBR_RS12180 0.56 +1.3 482,147 - BBR_RS12180 0.56 +0.4 482,147 - BBR_RS12180 0.56 +1.9 482,147 - BBR_RS12180 0.56 -0.1 482,147 - BBR_RS12180 0.56 +3.8 482,169 + BBR_RS12180 0.58 -0.4 482,170 - BBR_RS12180 0.58 -1.3 482,202 + BBR_RS12180 0.60 -2.0 482,202 + BBR_RS12180 0.60 +1.7 482,214 + BBR_RS12180 0.61 +0.0 482,232 + BBR_RS12180 0.63 +0.5 482,237 + BBR_RS12180 0.63 +0.9 482,240 - BBR_RS12180 0.63 +0.0 482,246 - BBR_RS12180 0.64 -0.2 482,255 - BBR_RS12180 0.64 +2.4 482,303 + BBR_RS12180 0.68 +1.7 482,305 + BBR_RS12180 0.68 -0.4 482,339 - BBR_RS12180 0.71 -1.5 482,339 - BBR_RS12180 0.71 -1.5 482,339 - BBR_RS12180 0.71 +1.9 482,339 - BBR_RS12180 0.71 -1.8 482,339 - BBR_RS12180 0.71 +0.7 482,347 + BBR_RS12180 0.71 -1.4 482,347 + BBR_RS12180 0.71 +0.2 482,376 + BBR_RS12180 0.73 +0.7 482,376 + BBR_RS12180 0.73 +0.9 482,376 + BBR_RS12180 0.73 -1.2 482,376 + BBR_RS12180 0.73 -0.2 482,376 + BBR_RS12180 0.73 -0.4 482,376 + BBR_RS12180 0.73 -0.7 482,376 + BBR_RS12180 0.73 -0.9 482,376 + BBR_RS12180 0.73 -0.7 482,376 + BBR_RS12180 0.73 -0.3 482,376 + BBR_RS12180 0.73 -1.2 482,378 + BBR_RS12180 0.74 -2.2 482,380 - BBR_RS12180 0.74 +1.5 482,384 - BBR_RS12180 0.74 -2.2 482,384 - BBR_RS12180 0.74 +0.1 482,384 - BBR_RS12180 0.74 -0.2 482,384 - BBR_RS12180 0.74 -0.8 482,384 - BBR_RS12180 0.74 +1.5 482,384 - BBR_RS12180 0.74 +0.5 482,384 - BBR_RS12180 0.74 -0.6 482,384 - BBR_RS12180 0.74 +0.3 482,384 - BBR_RS12180 0.74 -0.2 482,386 - BBR_RS12180 0.74 +2.1 482,388 + BBR_RS12180 0.74 -2.2 482,388 + BBR_RS12180 0.74 -0.2 482,390 - BBR_RS12180 0.75 +1.9 482,391 - BBR_RS12180 0.75 -1.8 482,409 + BBR_RS12180 0.76 +2.9 482,410 + BBR_RS12180 0.76 +1.4 482,410 + BBR_RS12180 0.76 -0.1 482,415 - BBR_RS12180 0.76 +0.3 482,433 - BBR_RS12180 0.78 +1.9 482,478 + BBR_RS12180 0.81 -0.0 482,478 + BBR_RS12180 0.81 +1.1 482,478 - BBR_RS12180 0.81 +2.5 482,486 - BBR_RS12180 0.82 -1.8 482,496 - BBR_RS12180 0.82 -1.3 482,524 - BBR_RS12180 0.85 +2.1 482,524 - BBR_RS12180 0.85 -0.5 482,538 - BBR_RS12180 0.86 +1.5 482,544 + BBR_RS12180 0.86 +0.3 482,557 - BBR_RS12180 0.87 +1.1 482,589 - BBR_RS12180 0.89 +0.6 482,604 + +1.2 482,604 + +3.0 482,604 + +4.1 482,604 + -0.8 482,604 + -0.5 482,609 + +0.5 482,612 - +0.1 482,622 - -0.6 482,622 - +2.2 482,622 - -0.3 482,706 + -1.2 482,714 - -0.6 482,726 + +0.4 482,726 + +0.7 482,750 + +0.3 482,771 - +1.5 482,789 - +1.8 482,798 + +0.4 482,820 + +0.5 482,829 + -0.4 482,857 + +1.3 482,871 - -1.1 482,871 - +0.6 482,884 - +1.4 482,928 + +0.6 482,928 + +0.5 482,928 + -0.6 482,928 + -3.0 482,928 + -1.2 482,933 + +2.4 482,936 - -0.5 482,936 - -1.5 482,936 - -2.3 482,936 - +0.4 482,936 - +1.0 482,936 - +1.5 482,936 - +1.8 482,938 - -0.7 482,943 - +0.2 482,943 - +1.6 482,997 + -1.2 482,997 + +1.4 483,005 - +1.8 483,016 - +1.7 483,039 + -0.2 483,039 + +0.2 483,046 - +0.5 483,047 + -0.5 483,051 + -1.8 483,052 + -0.4 483,054 - -1.7 483,060 - BBR_RS12185 0.11 -1.7 483,075 - BBR_RS12185 0.14 -1.7 483,107 + BBR_RS12185 0.19 +1.2 483,112 + BBR_RS12185 0.20 -2.0 483,141 - BBR_RS12185 0.25 -0.3 483,141 - BBR_RS12185 0.25 +0.6 483,147 + BBR_RS12185 0.26 +0.8 483,164 + BBR_RS12185 0.30 -1.1 483,164 - BBR_RS12185 0.30 +2.2 483,169 + BBR_RS12185 0.30 -1.9 483,187 + BBR_RS12185 0.34 -0.5 483,216 - BBR_RS12185 0.39 -0.9 483,226 - BBR_RS12185 0.41 +0.6 483,233 + BBR_RS12185 0.42 -1.5 483,233 + BBR_RS12185 0.42 -1.7 483,233 + BBR_RS12185 0.42 -1.0 483,233 + BBR_RS12185 0.42 +0.7 483,245 + BBR_RS12185 0.44 +1.8 483,293 + BBR_RS12185 0.53 -1.3 483,329 + BBR_RS12185 0.59 +1.0 483,335 + BBR_RS12185 0.60 +0.7 483,336 + BBR_RS12185 0.61 -0.8 483,343 - BBR_RS12185 0.62 +0.7 483,355 + BBR_RS12185 0.64 -1.3 483,363 - BBR_RS12185 0.65 +2.1 483,368 - BBR_RS12185 0.66 -1.7 483,370 - BBR_RS12185 0.67 +1.6 483,380 - BBR_RS12185 0.68 -0.2 483,384 + BBR_RS12185 0.69 +0.1 483,386 + BBR_RS12185 0.70 +0.2 483,398 + BBR_RS12185 0.72 +0.8 483,409 + BBR_RS12185 0.74 -0.5 483,425 - BBR_RS12185 0.77 +1.1 483,450 - BBR_RS12185 0.81 -0.2 483,460 + BBR_RS12185 0.83 -0.8 483,463 - BBR_RS12185 0.83 -1.2 483,463 - BBR_RS12185 0.83 -1.0 483,485 - BBR_RS12185 0.87 -0.9 483,486 + BBR_RS12185 0.88 -1.0 483,500 + -0.4 483,551 - -0.8 483,621 + -1.0 483,639 + -1.7 483,639 + +2.0 483,647 - -0.2 483,654 - -1.6 483,687 + +1.9 483,687 + +1.1 483,687 + +1.1 483,687 + -1.1 483,687 + -1.8 483,695 - -1.6 483,718 - -0.3 483,758 + +1.9 483,763 + -0.6 483,805 + -0.2 483,813 - +0.7 483,825 + +0.2 483,833 - +1.5 484,324 + -1.6 484,334 - +2.5 484,353 + -2.2 484,395 + +1.6 484,396 + +0.3 484,403 - +1.5 484,420 + +0.4 484,453 + -2.2 484,459 - +0.5 484,466 + +0.3 484,492 - -1.2 484,511 + +1.1 484,511 + +2.0 484,518 + BBR_RS12195 0.11 -1.0 484,518 + BBR_RS12195 0.11 +2.2 484,518 + BBR_RS12195 0.11 +0.8 484,544 + BBR_RS12195 0.16 -0.4 484,550 - BBR_RS12195 0.17 +0.7 484,551 + BBR_RS12195 0.17 +2.1 484,552 - BBR_RS12195 0.17 -1.5 484,552 - BBR_RS12195 0.17 +0.8 484,552 - BBR_RS12195 0.17 +0.4 484,554 - BBR_RS12195 0.18 -1.3
Or see this region's nucleotide sequence