Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS34965

Experiment: Carbon source D,L-Lactate 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS34960 and RR42_RS34965 are separated by 258 nucleotidesRR42_RS34965 and RR42_RS34970 are separated by 61 nucleotides RR42_RS34960: RR42_RS34960 - membrane protein, at 3,294,432 to 3,294,935 _RS34960 RR42_RS34965: RR42_RS34965 - dioxygenase, at 3,295,194 to 3,296,729 _RS34965 RR42_RS34970: RR42_RS34970 - bifunctional D-altronate/D-mannonate dehydratase, at 3,296,791 to 3,298,002 _RS34970 Position (kb) 3295 3296 3297Strain fitness (log2 ratio) -2 -1 0 1at 3294.307 kb on - strandat 3294.307 kb on - strandat 3294.441 kb on + strandat 3294.441 kb on + strandat 3294.441 kb on + strandat 3294.441 kb on + strandat 3294.442 kb on - strandat 3294.442 kb on - strandat 3294.512 kb on + strand, within RR42_RS34960at 3294.513 kb on - strand, within RR42_RS34960at 3294.513 kb on - strand, within RR42_RS34960at 3294.563 kb on - strand, within RR42_RS34960at 3294.563 kb on - strand, within RR42_RS34960at 3294.907 kb on + strandat 3294.936 kb on - strandat 3294.950 kb on - strandat 3295.014 kb on + strandat 3295.015 kb on - strandat 3295.015 kb on - strandat 3295.052 kb on - strandat 3295.064 kb on + strandat 3295.064 kb on + strandat 3295.064 kb on + strandat 3295.065 kb on - strandat 3295.065 kb on - strandat 3295.065 kb on - strandat 3295.065 kb on - strandat 3295.105 kb on + strandat 3295.105 kb on + strandat 3295.172 kb on + strandat 3295.172 kb on + strandat 3295.427 kb on + strand, within RR42_RS34965at 3295.595 kb on + strand, within RR42_RS34965at 3295.596 kb on - strand, within RR42_RS34965at 3295.596 kb on - strand, within RR42_RS34965at 3296.468 kb on - strand, within RR42_RS34965at 3296.676 kb on + strandat 3296.676 kb on + strandat 3296.676 kb on + strandat 3296.676 kb on + strandat 3296.677 kb on - strandat 3296.677 kb on - strandat 3296.792 kb on + strandat 3296.793 kb on - strandat 3296.840 kb on + strandat 3296.915 kb on + strand, within RR42_RS34970at 3296.984 kb on + strand, within RR42_RS34970at 3296.985 kb on - strand, within RR42_RS34970at 3297.162 kb on - strand, within RR42_RS34970at 3297.412 kb on + strand, within RR42_RS34970at 3297.413 kb on - strand, within RR42_RS34970at 3297.413 kb on - strand, within RR42_RS34970at 3297.528 kb on - strand, within RR42_RS34970at 3297.528 kb on - strand, within RR42_RS34970at 3297.528 kb on - strand, within RR42_RS34970at 3297.537 kb on + strand, within RR42_RS34970

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbon source D,L-Lactate 10 mM
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3,294,307 - +0.0
3,294,307 - +0.5
3,294,441 + +0.6
3,294,441 + -0.0
3,294,441 + -0.6
3,294,441 + -0.7
3,294,442 - -0.1
3,294,442 - -0.5
3,294,512 + RR42_RS34960 0.16 -0.1
3,294,513 - RR42_RS34960 0.16 +0.5
3,294,513 - RR42_RS34960 0.16 -0.4
3,294,563 - RR42_RS34960 0.26 -0.7
3,294,563 - RR42_RS34960 0.26 +0.0
3,294,907 + +0.5
3,294,936 - +0.1
3,294,950 - +0.8
3,295,014 + +0.2
3,295,015 - +0.4
3,295,015 - -0.2
3,295,052 - +0.4
3,295,064 + -0.4
3,295,064 + -0.1
3,295,064 + +0.8
3,295,065 - +0.2
3,295,065 - -0.1
3,295,065 - -0.8
3,295,065 - +0.0
3,295,105 + +1.0
3,295,105 + -0.3
3,295,172 + +0.4
3,295,172 + +0.3
3,295,427 + RR42_RS34965 0.15 +0.8
3,295,595 + RR42_RS34965 0.26 -0.8
3,295,596 - RR42_RS34965 0.26 +0.3
3,295,596 - RR42_RS34965 0.26 +0.6
3,296,468 - RR42_RS34965 0.83 +1.2
3,296,676 + -0.1
3,296,676 + +0.0
3,296,676 + -0.5
3,296,676 + +0.2
3,296,677 - -0.3
3,296,677 - +1.1
3,296,792 + -0.1
3,296,793 - +0.4
3,296,840 + +0.5
3,296,915 + RR42_RS34970 0.10 +0.3
3,296,984 + RR42_RS34970 0.16 -0.7
3,296,985 - RR42_RS34970 0.16 +0.1
3,297,162 - RR42_RS34970 0.31 +0.4
3,297,412 + RR42_RS34970 0.51 -0.3
3,297,413 - RR42_RS34970 0.51 -0.9
3,297,413 - RR42_RS34970 0.51 +0.1
3,297,528 - RR42_RS34970 0.61 +0.2
3,297,528 - RR42_RS34970 0.61 -0.9
3,297,528 - RR42_RS34970 0.61 -2.3
3,297,537 + RR42_RS34970 0.62 -0.2

Or see this region's nucleotide sequence