Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS15600

Experiment: Carbon source D,L-Lactate 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS15595 and RR42_RS15600 are separated by 76 nucleotidesRR42_RS15600 and RR42_RS15605 are separated by 525 nucleotides RR42_RS15595: RR42_RS15595 - acetoacetyl-CoA synthetase, at 3,368,532 to 3,370,568 _RS15595 RR42_RS15600: RR42_RS15600 - glutathione S-transferase, at 3,370,645 to 3,371,301 _RS15600 RR42_RS15605: RR42_RS15605 - poly(3-hydroxybutyrate) depolymerase, at 3,371,827 to 3,373,041 _RS15605 Position (kb) 3370 3371 3372Strain fitness (log2 ratio) -2 -1 0 1at 3369.719 kb on - strand, within RR42_RS15595at 3369.771 kb on + strand, within RR42_RS15595at 3369.771 kb on + strand, within RR42_RS15595at 3369.772 kb on - strand, within RR42_RS15595at 3369.879 kb on + strand, within RR42_RS15595at 3370.122 kb on + strand, within RR42_RS15595at 3370.123 kb on - strand, within RR42_RS15595at 3370.240 kb on - strand, within RR42_RS15595at 3370.240 kb on - strand, within RR42_RS15595at 3370.240 kb on - strand, within RR42_RS15595at 3370.257 kb on + strand, within RR42_RS15595at 3370.257 kb on + strand, within RR42_RS15595at 3370.258 kb on - strand, within RR42_RS15595at 3370.258 kb on - strand, within RR42_RS15595at 3370.258 kb on - strand, within RR42_RS15595at 3370.258 kb on - strand, within RR42_RS15595at 3370.259 kb on + strand, within RR42_RS15595at 3370.260 kb on - strand, within RR42_RS15595at 3370.260 kb on - strand, within RR42_RS15595at 3370.509 kb on + strandat 3370.510 kb on - strandat 3370.742 kb on + strand, within RR42_RS15600at 3370.775 kb on + strand, within RR42_RS15600at 3370.775 kb on + strand, within RR42_RS15600at 3370.776 kb on - strand, within RR42_RS15600at 3370.776 kb on - strand, within RR42_RS15600at 3370.806 kb on - strand, within RR42_RS15600at 3370.806 kb on - strand, within RR42_RS15600at 3370.845 kb on - strand, within RR42_RS15600at 3371.079 kb on - strand, within RR42_RS15600at 3371.272 kb on - strandat 3371.435 kb on + strandat 3371.436 kb on - strandat 3371.449 kb on - strandat 3371.451 kb on + strandat 3371.451 kb on + strandat 3371.451 kb on + strandat 3371.497 kb on + strandat 3371.591 kb on - strandat 3371.786 kb on + strandat 3371.811 kb on + strandat 3371.843 kb on - strandat 3371.851 kb on + strandat 3372.115 kb on + strand, within RR42_RS15605at 3372.214 kb on + strand, within RR42_RS15605at 3372.215 kb on - strand, within RR42_RS15605at 3372.215 kb on - strand, within RR42_RS15605at 3372.280 kb on + strand, within RR42_RS15605at 3372.286 kb on + strand, within RR42_RS15605at 3372.287 kb on - strand, within RR42_RS15605

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbon source D,L-Lactate 10 mM
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3,369,719 - RR42_RS15595 0.58 +0.4
3,369,771 + RR42_RS15595 0.61 -0.3
3,369,771 + RR42_RS15595 0.61 -1.3
3,369,772 - RR42_RS15595 0.61 -0.4
3,369,879 + RR42_RS15595 0.66 +0.9
3,370,122 + RR42_RS15595 0.78 +0.9
3,370,123 - RR42_RS15595 0.78 +0.3
3,370,240 - RR42_RS15595 0.84 -0.4
3,370,240 - RR42_RS15595 0.84 -0.3
3,370,240 - RR42_RS15595 0.84 -0.0
3,370,257 + RR42_RS15595 0.85 -1.2
3,370,257 + RR42_RS15595 0.85 -0.3
3,370,258 - RR42_RS15595 0.85 +0.9
3,370,258 - RR42_RS15595 0.85 -0.8
3,370,258 - RR42_RS15595 0.85 -0.3
3,370,258 - RR42_RS15595 0.85 +0.6
3,370,259 + RR42_RS15595 0.85 -0.1
3,370,260 - RR42_RS15595 0.85 -1.0
3,370,260 - RR42_RS15595 0.85 +0.3
3,370,509 + -0.1
3,370,510 - +0.3
3,370,742 + RR42_RS15600 0.15 -1.9
3,370,775 + RR42_RS15600 0.20 -0.5
3,370,775 + RR42_RS15600 0.20 +0.2
3,370,776 - RR42_RS15600 0.20 -1.1
3,370,776 - RR42_RS15600 0.20 -0.1
3,370,806 - RR42_RS15600 0.25 -0.1
3,370,806 - RR42_RS15600 0.25 -1.3
3,370,845 - RR42_RS15600 0.30 +0.1
3,371,079 - RR42_RS15600 0.66 -0.1
3,371,272 - +1.0
3,371,435 + +1.2
3,371,436 - +1.2
3,371,449 - -0.1
3,371,451 + +1.2
3,371,451 + +0.1
3,371,451 + -0.1
3,371,497 + +0.5
3,371,591 - -0.5
3,371,786 + +0.6
3,371,811 + -0.2
3,371,843 - +0.3
3,371,851 + -0.1
3,372,115 + RR42_RS15605 0.24 +0.8
3,372,214 + RR42_RS15605 0.32 -0.5
3,372,215 - RR42_RS15605 0.32 -0.1
3,372,215 - RR42_RS15605 0.32 +0.3
3,372,280 + RR42_RS15605 0.37 -0.4
3,372,286 + RR42_RS15605 0.38 +0.3
3,372,287 - RR42_RS15605 0.38 +0.5

Or see this region's nucleotide sequence