Experiment: Recovery broth with Norfloxacin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17745 and BBR_RS21015 are separated by 21 nucleotides BBR_RS21015 and BBR_RS17750 are separated by 285 nucleotides
BBR_RS17745: BBR_RS17745 - isoleucine--tRNA ligase, at 1,761,043 to 1,764,351
_RS17745
BBR_RS21015: BBR_RS21015 - hypothetical protein, at 1,764,373 to 1,764,738
_RS21015
BBR_RS17750: BBR_RS17750 - ribokinase, at 1,765,024 to 1,766,025
_RS17750
Position (kb)
1764
1765 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1764.373 kb on - strand at 1764.381 kb on - strand at 1764.521 kb on - strand, within BBR_RS21015 at 1764.684 kb on - strand, within BBR_RS21015 at 1764.727 kb on + strand at 1764.745 kb on + strand at 1764.767 kb on - strand at 1764.767 kb on - strand at 1764.796 kb on + strand at 1764.804 kb on + strand at 1764.804 kb on + strand at 1764.804 kb on - strand at 1764.811 kb on - strand at 1764.819 kb on - strand at 1764.821 kb on - strand at 1764.858 kb on - strand at 1764.858 kb on - strand at 1764.928 kb on + strand at 1764.928 kb on + strand at 1764.928 kb on + strand at 1764.930 kb on + strand at 1764.930 kb on + strand at 1764.936 kb on - strand at 1764.938 kb on - strand at 1764.938 kb on - strand at 1764.940 kb on + strand at 1764.943 kb on - strand at 1764.948 kb on - strand at 1764.950 kb on + strand at 1764.962 kb on + strand at 1765.001 kb on + strand at 1765.001 kb on + strand at 1765.001 kb on + strand at 1765.008 kb on + strand at 1765.008 kb on + strand at 1765.009 kb on - strand at 1765.009 kb on - strand at 1765.009 kb on - strand at 1765.016 kb on - strand at 1765.016 kb on - strand at 1765.088 kb on - strand at 1765.088 kb on - strand at 1765.110 kb on + strand at 1765.118 kb on - strand at 1765.125 kb on - strand, within BBR_RS17750 at 1765.125 kb on - strand, within BBR_RS17750 at 1765.125 kb on - strand, within BBR_RS17750 at 1765.141 kb on + strand, within BBR_RS17750 at 1765.149 kb on - strand, within BBR_RS17750 at 1765.149 kb on - strand, within BBR_RS17750 at 1765.170 kb on - strand, within BBR_RS17750 at 1765.217 kb on + strand, within BBR_RS17750 at 1765.218 kb on - strand, within BBR_RS17750 at 1765.260 kb on + strand, within BBR_RS17750 at 1765.296 kb on + strand, within BBR_RS17750 at 1765.329 kb on + strand, within BBR_RS17750 at 1765.329 kb on + strand, within BBR_RS17750 at 1765.337 kb on - strand, within BBR_RS17750 at 1765.339 kb on + strand, within BBR_RS17750 at 1765.347 kb on - strand, within BBR_RS17750 at 1765.347 kb on - strand, within BBR_RS17750 at 1765.352 kb on - strand, within BBR_RS17750 at 1765.357 kb on - strand, within BBR_RS17750 at 1765.437 kb on + strand, within BBR_RS17750 at 1765.452 kb on - strand, within BBR_RS17750 at 1765.457 kb on - strand, within BBR_RS17750 at 1765.499 kb on + strand, within BBR_RS17750 at 1765.540 kb on + strand, within BBR_RS17750 at 1765.549 kb on - strand, within BBR_RS17750 at 1765.549 kb on - strand, within BBR_RS17750 at 1765.549 kb on - strand, within BBR_RS17750 at 1765.586 kb on - strand, within BBR_RS17750 at 1765.617 kb on - strand, within BBR_RS17750 at 1765.720 kb on - strand, within BBR_RS17750 at 1765.722 kb on + strand, within BBR_RS17750 at 1765.730 kb on - strand, within BBR_RS17750
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Norfloxacin 0.2 mM remove 1,764,373 - +0.7 1,764,381 - -1.2 1,764,521 - BBR_RS21015 0.40 -2.2 1,764,684 - BBR_RS21015 0.85 +0.3 1,764,727 + +0.5 1,764,745 + -0.9 1,764,767 - +0.4 1,764,767 - +1.0 1,764,796 + +0.8 1,764,804 + +0.6 1,764,804 + +0.1 1,764,804 - -0.2 1,764,811 - -0.4 1,764,819 - -0.7 1,764,821 - +0.4 1,764,858 - +0.6 1,764,858 - +0.4 1,764,928 + -0.7 1,764,928 + +0.5 1,764,928 + -0.2 1,764,930 + +1.6 1,764,930 + -0.5 1,764,936 - +0.7 1,764,938 - -0.2 1,764,938 - +0.4 1,764,940 + -0.2 1,764,943 - +0.6 1,764,948 - -0.5 1,764,950 + -1.8 1,764,962 + +0.8 1,765,001 + -0.1 1,765,001 + -0.3 1,765,001 + +1.1 1,765,008 + -0.2 1,765,008 + -0.0 1,765,009 - +0.1 1,765,009 - -0.1 1,765,009 - +0.0 1,765,016 - -0.3 1,765,016 - +0.6 1,765,088 - -0.8 1,765,088 - -0.0 1,765,110 + +0.8 1,765,118 - -2.8 1,765,125 - BBR_RS17750 0.10 +1.0 1,765,125 - BBR_RS17750 0.10 -1.0 1,765,125 - BBR_RS17750 0.10 +0.2 1,765,141 + BBR_RS17750 0.12 -0.5 1,765,149 - BBR_RS17750 0.12 +0.2 1,765,149 - BBR_RS17750 0.12 +0.5 1,765,170 - BBR_RS17750 0.15 -0.7 1,765,217 + BBR_RS17750 0.19 -0.5 1,765,218 - BBR_RS17750 0.19 +0.0 1,765,260 + BBR_RS17750 0.24 +0.6 1,765,296 + BBR_RS17750 0.27 +1.0 1,765,329 + BBR_RS17750 0.30 -1.9 1,765,329 + BBR_RS17750 0.30 +0.3 1,765,337 - BBR_RS17750 0.31 +0.7 1,765,339 + BBR_RS17750 0.31 +0.5 1,765,347 - BBR_RS17750 0.32 -0.4 1,765,347 - BBR_RS17750 0.32 -0.6 1,765,352 - BBR_RS17750 0.33 -2.2 1,765,357 - BBR_RS17750 0.33 -0.4 1,765,437 + BBR_RS17750 0.41 -0.1 1,765,452 - BBR_RS17750 0.43 -0.4 1,765,457 - BBR_RS17750 0.43 +1.0 1,765,499 + BBR_RS17750 0.47 +1.0 1,765,540 + BBR_RS17750 0.51 +0.6 1,765,549 - BBR_RS17750 0.52 -0.6 1,765,549 - BBR_RS17750 0.52 -0.7 1,765,549 - BBR_RS17750 0.52 +2.0 1,765,586 - BBR_RS17750 0.56 +0.7 1,765,617 - BBR_RS17750 0.59 -0.7 1,765,720 - BBR_RS17750 0.69 +0.1 1,765,722 + BBR_RS17750 0.70 +0.1 1,765,730 - BBR_RS17750 0.70 +0.4
Or see this region's nucleotide sequence