Experiment: Recovery broth with Norfloxacin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS18750 and BBR_RS18755 are separated by 92 nucleotides BBR_RS18755 and BBR_RS18760 are separated by 270 nucleotides
BBR_RS18750: BBR_RS18750 - pseudouridine synthase, at 1,997,509 to 1,998,663
_RS18750
BBR_RS18755: BBR_RS18755 - 30S ribosome-binding factor RbfA, at 1,998,756 to 1,999,241
_RS18755
BBR_RS18760: BBR_RS18760 - translation initiation factor IF-2, at 1,999,512 to 2,002,334
_RS18760
Position (kb)
1998
1999
2000 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1997.763 kb on + strand, within BBR_RS18750 at 1997.763 kb on - strand, within BBR_RS18750 at 1997.771 kb on - strand, within BBR_RS18750 at 1997.785 kb on + strand, within BBR_RS18750 at 1997.785 kb on - strand, within BBR_RS18750 at 1997.793 kb on - strand, within BBR_RS18750 at 1997.793 kb on - strand, within BBR_RS18750 at 1997.793 kb on - strand, within BBR_RS18750 at 1997.837 kb on + strand, within BBR_RS18750 at 1997.845 kb on - strand, within BBR_RS18750 at 1997.850 kb on + strand, within BBR_RS18750 at 1997.850 kb on + strand, within BBR_RS18750 at 1997.852 kb on + strand, within BBR_RS18750 at 1997.857 kb on + strand, within BBR_RS18750 at 1997.857 kb on + strand, within BBR_RS18750 at 1997.858 kb on - strand, within BBR_RS18750 at 1997.882 kb on + strand, within BBR_RS18750 at 1997.882 kb on + strand, within BBR_RS18750 at 1997.882 kb on + strand, within BBR_RS18750 at 1997.890 kb on - strand, within BBR_RS18750 at 1997.894 kb on - strand, within BBR_RS18750 at 1997.923 kb on + strand, within BBR_RS18750 at 1997.928 kb on + strand, within BBR_RS18750 at 1997.928 kb on - strand, within BBR_RS18750 at 1997.938 kb on + strand, within BBR_RS18750 at 1997.955 kb on - strand, within BBR_RS18750 at 1997.955 kb on - strand, within BBR_RS18750 at 1997.971 kb on - strand, within BBR_RS18750 at 1998.001 kb on + strand, within BBR_RS18750 at 1998.025 kb on + strand, within BBR_RS18750 at 1998.038 kb on + strand, within BBR_RS18750 at 1998.038 kb on + strand, within BBR_RS18750 at 1998.077 kb on + strand, within BBR_RS18750 at 1998.085 kb on - strand, within BBR_RS18750 at 1998.085 kb on - strand, within BBR_RS18750 at 1998.107 kb on - strand, within BBR_RS18750 at 1998.152 kb on + strand, within BBR_RS18750 at 1998.153 kb on + strand, within BBR_RS18750 at 1998.160 kb on + strand, within BBR_RS18750 at 1998.160 kb on - strand, within BBR_RS18750 at 1998.165 kb on + strand, within BBR_RS18750 at 1998.168 kb on - strand, within BBR_RS18750 at 1998.168 kb on - strand, within BBR_RS18750 at 1998.251 kb on - strand, within BBR_RS18750 at 1998.266 kb on + strand, within BBR_RS18750 at 1998.274 kb on - strand, within BBR_RS18750 at 1998.286 kb on - strand, within BBR_RS18750 at 1998.299 kb on - strand, within BBR_RS18750 at 1998.343 kb on - strand, within BBR_RS18750 at 1998.343 kb on - strand, within BBR_RS18750 at 1998.343 kb on - strand, within BBR_RS18750 at 1998.365 kb on - strand, within BBR_RS18750 at 1998.365 kb on - strand, within BBR_RS18750 at 1998.384 kb on - strand, within BBR_RS18750 at 1998.422 kb on - strand, within BBR_RS18750 at 1998.432 kb on - strand, within BBR_RS18750 at 1998.432 kb on - strand, within BBR_RS18750 at 1998.437 kb on - strand, within BBR_RS18750 at 1998.457 kb on - strand, within BBR_RS18750 at 1998.463 kb on - strand, within BBR_RS18750 at 1998.468 kb on - strand, within BBR_RS18750 at 1998.476 kb on + strand, within BBR_RS18750 at 1998.488 kb on + strand, within BBR_RS18750 at 1998.519 kb on - strand, within BBR_RS18750 at 1998.542 kb on - strand, within BBR_RS18750 at 1998.629 kb on + strand at 1998.651 kb on - strand at 1998.661 kb on + strand at 1998.671 kb on + strand at 1998.706 kb on - strand at 1998.714 kb on + strand at 1998.715 kb on + strand at 1998.722 kb on - strand at 1998.722 kb on - strand at 1998.722 kb on - strand at 1998.723 kb on - strand at 1998.723 kb on - strand at 1998.780 kb on - strand at 1998.780 kb on - strand at 1998.782 kb on - strand at 1998.784 kb on + strand at 1998.825 kb on - strand, within BBR_RS18755 at 1998.826 kb on - strand, within BBR_RS18755 at 1998.846 kb on - strand, within BBR_RS18755 at 1999.301 kb on - strand at 1999.308 kb on - strand at 1999.316 kb on - strand at 1999.321 kb on - strand at 1999.321 kb on - strand at 1999.321 kb on - strand at 1999.323 kb on - strand at 1999.335 kb on + strand at 1999.336 kb on - strand at 1999.343 kb on - strand at 1999.343 kb on - strand at 1999.343 kb on - strand at 1999.345 kb on - strand at 1999.455 kb on + strand at 1999.467 kb on - strand at 1999.471 kb on - strand at 1999.498 kb on - strand at 1999.504 kb on - strand at 1999.518 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Norfloxacin 0.2 mM remove 1,997,763 + BBR_RS18750 0.22 -1.0 1,997,763 - BBR_RS18750 0.22 -0.4 1,997,771 - BBR_RS18750 0.23 -0.4 1,997,785 + BBR_RS18750 0.24 -0.7 1,997,785 - BBR_RS18750 0.24 +0.1 1,997,793 - BBR_RS18750 0.25 +0.7 1,997,793 - BBR_RS18750 0.25 -0.1 1,997,793 - BBR_RS18750 0.25 -2.2 1,997,837 + BBR_RS18750 0.28 +0.8 1,997,845 - BBR_RS18750 0.29 -0.4 1,997,850 + BBR_RS18750 0.30 -0.3 1,997,850 + BBR_RS18750 0.30 +0.4 1,997,852 + BBR_RS18750 0.30 -0.2 1,997,857 + BBR_RS18750 0.30 +1.6 1,997,857 + BBR_RS18750 0.30 +0.5 1,997,858 - BBR_RS18750 0.30 +0.3 1,997,882 + BBR_RS18750 0.32 +0.1 1,997,882 + BBR_RS18750 0.32 -0.9 1,997,882 + BBR_RS18750 0.32 +0.8 1,997,890 - BBR_RS18750 0.33 -0.8 1,997,894 - BBR_RS18750 0.33 +0.5 1,997,923 + BBR_RS18750 0.36 +1.5 1,997,928 + BBR_RS18750 0.36 -0.8 1,997,928 - BBR_RS18750 0.36 -0.1 1,997,938 + BBR_RS18750 0.37 -0.5 1,997,955 - BBR_RS18750 0.39 -0.3 1,997,955 - BBR_RS18750 0.39 -0.1 1,997,971 - BBR_RS18750 0.40 +0.8 1,998,001 + BBR_RS18750 0.43 -0.9 1,998,025 + BBR_RS18750 0.45 +2.0 1,998,038 + BBR_RS18750 0.46 +0.2 1,998,038 + BBR_RS18750 0.46 +0.5 1,998,077 + BBR_RS18750 0.49 +0.4 1,998,085 - BBR_RS18750 0.50 +0.6 1,998,085 - BBR_RS18750 0.50 -0.3 1,998,107 - BBR_RS18750 0.52 +1.4 1,998,152 + BBR_RS18750 0.56 -0.3 1,998,153 + BBR_RS18750 0.56 +0.1 1,998,160 + BBR_RS18750 0.56 -0.7 1,998,160 - BBR_RS18750 0.56 +0.0 1,998,165 + BBR_RS18750 0.57 -0.2 1,998,168 - BBR_RS18750 0.57 -0.9 1,998,168 - BBR_RS18750 0.57 +0.5 1,998,251 - BBR_RS18750 0.64 +0.8 1,998,266 + BBR_RS18750 0.66 +1.0 1,998,274 - BBR_RS18750 0.66 -0.6 1,998,286 - BBR_RS18750 0.67 -1.0 1,998,299 - BBR_RS18750 0.68 -0.4 1,998,343 - BBR_RS18750 0.72 -0.8 1,998,343 - BBR_RS18750 0.72 +0.1 1,998,343 - BBR_RS18750 0.72 -0.7 1,998,365 - BBR_RS18750 0.74 -0.0 1,998,365 - BBR_RS18750 0.74 +0.3 1,998,384 - BBR_RS18750 0.76 -1.0 1,998,422 - BBR_RS18750 0.79 -1.1 1,998,432 - BBR_RS18750 0.80 -1.0 1,998,432 - BBR_RS18750 0.80 -0.4 1,998,437 - BBR_RS18750 0.80 -0.0 1,998,457 - BBR_RS18750 0.82 +2.4 1,998,463 - BBR_RS18750 0.83 +0.1 1,998,468 - BBR_RS18750 0.83 -0.0 1,998,476 + BBR_RS18750 0.84 +0.4 1,998,488 + BBR_RS18750 0.85 -0.2 1,998,519 - BBR_RS18750 0.87 +0.2 1,998,542 - BBR_RS18750 0.89 -0.8 1,998,629 + -0.3 1,998,651 - -1.5 1,998,661 + -1.1 1,998,671 + +0.4 1,998,706 - -0.7 1,998,714 + +0.6 1,998,715 + -0.7 1,998,722 - -0.1 1,998,722 - +0.1 1,998,722 - +0.4 1,998,723 - -0.2 1,998,723 - +0.1 1,998,780 - +1.1 1,998,780 - -0.6 1,998,782 - +0.7 1,998,784 + +0.2 1,998,825 - BBR_RS18755 0.14 +0.5 1,998,826 - BBR_RS18755 0.14 -0.4 1,998,846 - BBR_RS18755 0.19 -1.9 1,999,301 - -0.7 1,999,308 - -1.3 1,999,316 - +0.4 1,999,321 - -0.4 1,999,321 - -0.5 1,999,321 - +1.9 1,999,323 - +1.1 1,999,335 + +0.9 1,999,336 - -1.2 1,999,343 - -0.1 1,999,343 - -0.2 1,999,343 - -0.6 1,999,345 - +0.1 1,999,455 + +0.8 1,999,467 - +0.2 1,999,471 - -1.5 1,999,498 - +0.4 1,999,504 - +0.2 1,999,518 - +0.5
Or see this region's nucleotide sequence