Experiment: Recovery broth with Norfloxacin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15440 and BBR_RS15445 are separated by 151 nucleotides BBR_RS15445 and BBR_RS15450 are separated by 2 nucleotides BBR_RS15450 and BBR_RS15455 are separated by 3 nucleotides BBR_RS15455 and BBR_RS15460 are separated by 107 nucleotides
BBR_RS15440: BBR_RS15440 - orotidine-5'-phosphate decarboxylase, at 1,235,672 to 1,236,625
_RS15440
BBR_RS15445: BBR_RS15445 - dihydroorotate dehydrogenase electron transfer subunit, at 1,236,777 to 1,237,601
_RS15445
BBR_RS15450: BBR_RS15450 - dihydroorotate dehydrogenase, at 1,237,604 to 1,238,575
_RS15450
BBR_RS15455: BBR_RS15455 - orotate phosphoribosyltransferase, at 1,238,579 to 1,239,274
_RS15455
BBR_RS15460: BBR_RS15460 - hypothetical protein, at 1,239,382 to 1,239,570
_RS15460
Position (kb) 1237
1238
1239 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1236.653 kb on + strand at 1236.796 kb on + strand at 1236.917 kb on - strand, within BBR_RS15445 at 1236.923 kb on + strand, within BBR_RS15445 at 1236.937 kb on - strand, within BBR_RS15445 at 1236.956 kb on + strand, within BBR_RS15445 at 1237.007 kb on + strand, within BBR_RS15445 at 1237.007 kb on + strand, within BBR_RS15445 at 1237.030 kb on - strand, within BBR_RS15445 at 1237.062 kb on - strand, within BBR_RS15445 at 1237.187 kb on - strand, within BBR_RS15445 at 1237.192 kb on - strand, within BBR_RS15445 at 1237.192 kb on - strand, within BBR_RS15445 at 1237.194 kb on + strand, within BBR_RS15445 at 1237.281 kb on - strand, within BBR_RS15445 at 1237.308 kb on + strand, within BBR_RS15445 at 1237.405 kb on - strand, within BBR_RS15445 at 1237.426 kb on - strand, within BBR_RS15445 at 1237.426 kb on - strand, within BBR_RS15445 at 1237.496 kb on - strand, within BBR_RS15445 at 1237.529 kb on - strand at 1237.573 kb on + strand at 1237.596 kb on - strand at 1237.606 kb on - strand at 1237.623 kb on - strand at 1237.625 kb on + strand at 1237.630 kb on + strand at 1237.645 kb on + strand at 1237.688 kb on - strand at 1237.724 kb on + strand, within BBR_RS15450 at 1237.910 kb on + strand, within BBR_RS15450 at 1237.986 kb on + strand, within BBR_RS15450 at 1238.233 kb on - strand, within BBR_RS15450 at 1238.254 kb on - strand, within BBR_RS15450 at 1238.348 kb on - strand, within BBR_RS15450 at 1238.406 kb on + strand, within BBR_RS15450 at 1238.425 kb on + strand, within BBR_RS15450 at 1238.556 kb on + strand at 1238.652 kb on + strand, within BBR_RS15455 at 1238.866 kb on + strand, within BBR_RS15455 at 1238.866 kb on + strand, within BBR_RS15455 at 1238.941 kb on + strand, within BBR_RS15455 at 1239.030 kb on - strand, within BBR_RS15455 at 1239.384 kb on + strand at 1239.398 kb on + strand at 1239.398 kb on + strand at 1239.398 kb on + strand at 1239.404 kb on - strand, within BBR_RS15460 at 1239.406 kb on - strand, within BBR_RS15460 at 1239.406 kb on - strand, within BBR_RS15460 at 1239.406 kb on - strand, within BBR_RS15460 at 1239.485 kb on - strand, within BBR_RS15460 at 1239.560 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Norfloxacin 0.2 mM remove 1,236,653 + +0.1 1,236,796 + -1.5 1,236,917 - BBR_RS15445 0.17 -1.4 1,236,923 + BBR_RS15445 0.18 -1.1 1,236,937 - BBR_RS15445 0.19 +0.1 1,236,956 + BBR_RS15445 0.22 +0.8 1,237,007 + BBR_RS15445 0.28 +0.2 1,237,007 + BBR_RS15445 0.28 -0.1 1,237,030 - BBR_RS15445 0.31 -0.5 1,237,062 - BBR_RS15445 0.35 +1.0 1,237,187 - BBR_RS15445 0.50 -0.7 1,237,192 - BBR_RS15445 0.50 -0.2 1,237,192 - BBR_RS15445 0.50 -0.9 1,237,194 + BBR_RS15445 0.51 -0.0 1,237,281 - BBR_RS15445 0.61 +0.8 1,237,308 + BBR_RS15445 0.64 +1.6 1,237,405 - BBR_RS15445 0.76 +1.3 1,237,426 - BBR_RS15445 0.79 -0.4 1,237,426 - BBR_RS15445 0.79 +0.4 1,237,496 - BBR_RS15445 0.87 +2.1 1,237,529 - +1.0 1,237,573 + +0.9 1,237,596 - +0.4 1,237,606 - -0.6 1,237,623 - +0.6 1,237,625 + -0.4 1,237,630 + +0.6 1,237,645 + -1.0 1,237,688 - -1.7 1,237,724 + BBR_RS15450 0.12 -0.8 1,237,910 + BBR_RS15450 0.31 -0.5 1,237,986 + BBR_RS15450 0.39 +0.9 1,238,233 - BBR_RS15450 0.65 +3.1 1,238,254 - BBR_RS15450 0.67 -1.0 1,238,348 - BBR_RS15450 0.77 +1.1 1,238,406 + BBR_RS15450 0.83 -2.2 1,238,425 + BBR_RS15450 0.84 -1.1 1,238,556 + +0.9 1,238,652 + BBR_RS15455 0.10 -0.1 1,238,866 + BBR_RS15455 0.41 -0.3 1,238,866 + BBR_RS15455 0.41 -0.1 1,238,941 + BBR_RS15455 0.52 +0.2 1,239,030 - BBR_RS15455 0.65 +0.9 1,239,384 + -1.3 1,239,398 + +0.5 1,239,398 + -0.0 1,239,398 + +0.4 1,239,404 - BBR_RS15460 0.12 +1.2 1,239,406 - BBR_RS15460 0.13 -0.6 1,239,406 - BBR_RS15460 0.13 +0.4 1,239,406 - BBR_RS15460 0.13 -0.3 1,239,485 - BBR_RS15460 0.54 -0.8 1,239,560 - +0.6
Or see this region's nucleotide sequence