Experiment: Recovery broth with Norfloxacin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15420 and BBR_RS15425 are separated by 310 nucleotides BBR_RS15425 and BBR_RS15430 overlap by 1 nucleotides BBR_RS15430 and BBR_RS15435 overlap by 4 nucleotides
BBR_RS15420: BBR_RS15420 - bifunctional glutamine-synthetase adenylyltransferase/deadenyltransferase, at 1,229,269 to 1,232,451
_RS15420
BBR_RS15425: BBR_RS15425 - aspartate carbamoyltransferase, at 1,232,762 to 1,233,724
_RS15425
BBR_RS15430: BBR_RS15430 - aspartate carbamoyltransferase regulatory subunit, at 1,233,724 to 1,234,143
_RS15430
BBR_RS15435: BBR_RS15435 - dihydroorotase, at 1,234,140 to 1,235,654
_RS15435
Position (kb)
1232
1233
1234 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1231.764 kb on - strand, within BBR_RS15420 at 1231.774 kb on + strand, within BBR_RS15420 at 1231.788 kb on - strand, within BBR_RS15420 at 1231.806 kb on - strand, within BBR_RS15420 at 1231.812 kb on + strand, within BBR_RS15420 at 1231.888 kb on + strand, within BBR_RS15420 at 1231.999 kb on + strand, within BBR_RS15420 at 1232.021 kb on - strand, within BBR_RS15420 at 1232.035 kb on - strand, within BBR_RS15420 at 1232.137 kb on + strand at 1232.160 kb on + strand at 1232.166 kb on - strand at 1232.216 kb on + strand at 1232.216 kb on + strand at 1232.307 kb on + strand at 1232.360 kb on - strand at 1232.364 kb on + strand at 1232.428 kb on + strand at 1232.428 kb on + strand at 1232.453 kb on - strand at 1232.462 kb on - strand at 1232.646 kb on + strand at 1232.646 kb on + strand at 1232.882 kb on + strand, within BBR_RS15425 at 1232.923 kb on - strand, within BBR_RS15425 at 1233.009 kb on - strand, within BBR_RS15425 at 1233.156 kb on - strand, within BBR_RS15425 at 1233.156 kb on - strand, within BBR_RS15425 at 1233.333 kb on - strand, within BBR_RS15425 at 1233.535 kb on + strand, within BBR_RS15425 at 1233.622 kb on + strand, within BBR_RS15425 at 1233.702 kb on + strand at 1234.025 kb on + strand, within BBR_RS15430 at 1234.138 kb on - strand at 1234.570 kb on + strand, within BBR_RS15435 at 1234.578 kb on - strand, within BBR_RS15435 at 1234.667 kb on + strand, within BBR_RS15435 at 1234.667 kb on + strand, within BBR_RS15435
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Norfloxacin 0.2 mM remove 1,231,764 - BBR_RS15420 0.78 +0.2 1,231,774 + BBR_RS15420 0.79 -1.0 1,231,788 - BBR_RS15420 0.79 +0.0 1,231,806 - BBR_RS15420 0.80 +0.5 1,231,812 + BBR_RS15420 0.80 -0.1 1,231,888 + BBR_RS15420 0.82 +0.8 1,231,999 + BBR_RS15420 0.86 -0.3 1,232,021 - BBR_RS15420 0.86 -0.0 1,232,035 - BBR_RS15420 0.87 -0.7 1,232,137 + -0.1 1,232,160 + -1.0 1,232,166 - +1.0 1,232,216 + +0.1 1,232,216 + -0.8 1,232,307 + -0.1 1,232,360 - -0.3 1,232,364 + +0.4 1,232,428 + +0.7 1,232,428 + -0.7 1,232,453 - +0.2 1,232,462 - +1.0 1,232,646 + -0.6 1,232,646 + +0.9 1,232,882 + BBR_RS15425 0.12 +1.3 1,232,923 - BBR_RS15425 0.17 -0.4 1,233,009 - BBR_RS15425 0.26 +0.9 1,233,156 - BBR_RS15425 0.41 +0.9 1,233,156 - BBR_RS15425 0.41 -0.3 1,233,333 - BBR_RS15425 0.59 +0.2 1,233,535 + BBR_RS15425 0.80 +2.8 1,233,622 + BBR_RS15425 0.89 +0.8 1,233,702 + +0.2 1,234,025 + BBR_RS15430 0.72 +0.8 1,234,138 - -0.2 1,234,570 + BBR_RS15435 0.28 -0.1 1,234,578 - BBR_RS15435 0.29 +0.0 1,234,667 + BBR_RS15435 0.35 -1.6 1,234,667 + BBR_RS15435 0.35 +0.6
Or see this region's nucleotide sequence