Experiment: Recovery broth with Norfloxacin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15230 and BBR_RS15235 overlap by 1 nucleotides BBR_RS15235 and BBR_RS20885 are separated by 469 nucleotides BBR_RS20885 and BBR_RS15240 are separated by 33 nucleotides
BBR_RS15230: BBR_RS15230 - elongation factor 4, at 1,184,795 to 1,186,675
_RS15230
BBR_RS15235: BBR_RS15235 - coproporphyrinogen III oxidase, at 1,186,675 to 1,187,988
_RS15235
BBR_RS20885: BBR_RS20885 - hypothetical protein, at 1,188,458 to 1,188,664
_RS20885
BBR_RS15240: BBR_RS15240 - LacI family DNA-binding transcriptional regulator, at 1,188,698 to 1,189,726
_RS15240
Position (kb)
1186
1187
1188 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1185.705 kb on - strand, within BBR_RS15230 at 1185.807 kb on - strand, within BBR_RS15230 at 1185.909 kb on - strand, within BBR_RS15230 at 1185.914 kb on - strand, within BBR_RS15230 at 1185.924 kb on - strand, within BBR_RS15230 at 1185.978 kb on + strand, within BBR_RS15230 at 1186.126 kb on + strand, within BBR_RS15230 at 1186.126 kb on + strand, within BBR_RS15230 at 1186.267 kb on + strand, within BBR_RS15230 at 1186.452 kb on + strand, within BBR_RS15230 at 1186.462 kb on + strand, within BBR_RS15230 at 1186.470 kb on - strand, within BBR_RS15230 at 1186.563 kb on + strand at 1186.598 kb on + strand at 1186.606 kb on - strand at 1186.640 kb on + strand at 1186.655 kb on + strand at 1186.699 kb on - strand at 1186.731 kb on + strand at 1186.789 kb on + strand at 1187.087 kb on - strand, within BBR_RS15235 at 1187.094 kb on - strand, within BBR_RS15235 at 1187.107 kb on - strand, within BBR_RS15235 at 1187.183 kb on + strand, within BBR_RS15235 at 1187.203 kb on - strand, within BBR_RS15235 at 1187.396 kb on + strand, within BBR_RS15235 at 1187.397 kb on + strand, within BBR_RS15235 at 1187.404 kb on - strand, within BBR_RS15235 at 1187.452 kb on - strand, within BBR_RS15235 at 1187.462 kb on + strand, within BBR_RS15235 at 1187.485 kb on - strand, within BBR_RS15235 at 1187.500 kb on + strand, within BBR_RS15235 at 1187.508 kb on - strand, within BBR_RS15235 at 1187.601 kb on - strand, within BBR_RS15235 at 1187.711 kb on - strand, within BBR_RS15235 at 1187.767 kb on - strand, within BBR_RS15235 at 1187.797 kb on - strand, within BBR_RS15235 at 1187.845 kb on - strand, within BBR_RS15235 at 1187.873 kb on - strand at 1188.603 kb on + strand, within BBR_RS20885 at 1188.688 kb on + strand at 1188.716 kb on - strand at 1188.741 kb on + strand at 1188.797 kb on + strand at 1188.799 kb on - strand at 1188.807 kb on - strand, within BBR_RS15240 at 1188.853 kb on - strand, within BBR_RS15240
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Norfloxacin 0.2 mM remove 1,185,705 - BBR_RS15230 0.48 +1.8 1,185,807 - BBR_RS15230 0.54 +1.3 1,185,909 - BBR_RS15230 0.59 -0.5 1,185,914 - BBR_RS15230 0.59 -0.6 1,185,924 - BBR_RS15230 0.60 +0.1 1,185,978 + BBR_RS15230 0.63 -0.5 1,186,126 + BBR_RS15230 0.71 +2.0 1,186,126 + BBR_RS15230 0.71 +1.2 1,186,267 + BBR_RS15230 0.78 +0.5 1,186,452 + BBR_RS15230 0.88 -0.7 1,186,462 + BBR_RS15230 0.89 +0.2 1,186,470 - BBR_RS15230 0.89 +0.5 1,186,563 + -0.4 1,186,598 + +1.3 1,186,606 - +1.8 1,186,640 + +1.5 1,186,655 + -0.6 1,186,699 - -0.7 1,186,731 + -0.9 1,186,789 + -1.4 1,187,087 - BBR_RS15235 0.31 -1.4 1,187,094 - BBR_RS15235 0.32 -1.0 1,187,107 - BBR_RS15235 0.33 -0.7 1,187,183 + BBR_RS15235 0.39 -1.4 1,187,203 - BBR_RS15235 0.40 +1.0 1,187,396 + BBR_RS15235 0.55 -0.7 1,187,397 + BBR_RS15235 0.55 +1.0 1,187,404 - BBR_RS15235 0.55 -0.2 1,187,452 - BBR_RS15235 0.59 -0.4 1,187,462 + BBR_RS15235 0.60 +0.7 1,187,485 - BBR_RS15235 0.62 +0.6 1,187,500 + BBR_RS15235 0.63 -1.8 1,187,508 - BBR_RS15235 0.63 -0.3 1,187,601 - BBR_RS15235 0.70 -0.8 1,187,711 - BBR_RS15235 0.79 +2.0 1,187,767 - BBR_RS15235 0.83 +0.2 1,187,797 - BBR_RS15235 0.85 -0.8 1,187,845 - BBR_RS15235 0.89 +0.7 1,187,873 - +0.3 1,188,603 + BBR_RS20885 0.70 +0.2 1,188,688 + -0.3 1,188,716 - -0.6 1,188,741 + +0.9 1,188,797 + -0.1 1,188,799 - +0.8 1,188,807 - BBR_RS15240 0.11 +0.5 1,188,853 - BBR_RS15240 0.15 +0.5
Or see this region's nucleotide sequence