Experiment: Recovery broth with Norfloxacin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14190 and BBR_RS14200 are separated by 260 nucleotides BBR_RS14200 and BBR_RS14205 are separated by 45 nucleotides BBR_RS14205 and BBR_RS14210 overlap by 4 nucleotides
BBR_RS14190: BBR_RS14190 - transcriptional regulator, at 958,503 to 959,837
_RS14190
BBR_RS14200: BBR_RS14200 - WhiB family transcriptional regulator, at 960,098 to 960,397
_RS14200
BBR_RS14205: BBR_RS14205 - hypothetical protein, at 960,443 to 963,535
_RS14205
BBR_RS14210: BBR_RS14210 - hypothetical protein, at 963,532 to 965,073
_RS14210
Position (kb) 960
961
962
963
964 Strain fitness (log2 ratio) -2
-1
0
1
2 at 959.444 kb on + strand, within BBR_RS14190 at 959.444 kb on - strand, within BBR_RS14190 at 959.450 kb on + strand, within BBR_RS14190 at 959.452 kb on - strand, within BBR_RS14190 at 959.481 kb on - strand, within BBR_RS14190 at 959.491 kb on - strand, within BBR_RS14190 at 959.527 kb on - strand, within BBR_RS14190 at 959.575 kb on + strand, within BBR_RS14190 at 959.597 kb on + strand, within BBR_RS14190 at 959.597 kb on + strand, within BBR_RS14190 at 959.605 kb on - strand, within BBR_RS14190 at 959.612 kb on + strand, within BBR_RS14190 at 959.620 kb on - strand, within BBR_RS14190 at 959.637 kb on - strand, within BBR_RS14190 at 959.652 kb on - strand, within BBR_RS14190 at 959.688 kb on - strand, within BBR_RS14190 at 959.735 kb on - strand at 959.758 kb on - strand at 959.908 kb on - strand at 959.929 kb on - strand at 959.932 kb on + strand at 959.964 kb on - strand at 960.142 kb on + strand, within BBR_RS14200 at 960.142 kb on + strand, within BBR_RS14200 at 960.150 kb on - strand, within BBR_RS14200 at 960.154 kb on + strand, within BBR_RS14200 at 960.154 kb on + strand, within BBR_RS14200 at 960.162 kb on - strand, within BBR_RS14200 at 960.162 kb on - strand, within BBR_RS14200 at 960.195 kb on + strand, within BBR_RS14200 at 960.205 kb on + strand, within BBR_RS14200 at 960.342 kb on + strand, within BBR_RS14200 at 960.350 kb on - strand, within BBR_RS14200 at 960.379 kb on + strand at 960.397 kb on + strand at 960.439 kb on - strand at 960.516 kb on + strand at 960.946 kb on - strand, within BBR_RS14205 at 961.366 kb on + strand, within BBR_RS14205 at 961.838 kb on - strand, within BBR_RS14205 at 962.195 kb on - strand, within BBR_RS14205 at 962.742 kb on - strand, within BBR_RS14205 at 962.794 kb on + strand, within BBR_RS14205 at 962.802 kb on - strand, within BBR_RS14205 at 962.827 kb on + strand, within BBR_RS14205 at 962.830 kb on + strand, within BBR_RS14205 at 962.836 kb on + strand, within BBR_RS14205 at 962.946 kb on + strand, within BBR_RS14205 at 963.245 kb on + strand at 963.275 kb on - strand at 963.383 kb on + strand at 963.391 kb on + strand at 963.391 kb on + strand at 963.392 kb on + strand at 963.399 kb on - strand at 963.414 kb on + strand at 963.422 kb on - strand at 963.456 kb on - strand at 963.483 kb on - strand at 963.495 kb on + strand at 963.516 kb on + strand at 963.555 kb on + strand at 963.709 kb on - strand, within BBR_RS14210 at 963.993 kb on - strand, within BBR_RS14210 at 964.075 kb on + strand, within BBR_RS14210 at 964.448 kb on - strand, within BBR_RS14210 at 964.526 kb on - strand, within BBR_RS14210
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Norfloxacin 0.2 mM remove 959,444 + BBR_RS14190 0.70 -0.7 959,444 - BBR_RS14190 0.70 +0.4 959,450 + BBR_RS14190 0.71 -1.0 959,452 - BBR_RS14190 0.71 -1.8 959,481 - BBR_RS14190 0.73 -0.6 959,491 - BBR_RS14190 0.74 -2.5 959,527 - BBR_RS14190 0.77 -0.6 959,575 + BBR_RS14190 0.80 -0.2 959,597 + BBR_RS14190 0.82 -1.2 959,597 + BBR_RS14190 0.82 -2.2 959,605 - BBR_RS14190 0.83 +1.0 959,612 + BBR_RS14190 0.83 -0.4 959,620 - BBR_RS14190 0.84 -1.7 959,637 - BBR_RS14190 0.85 +0.7 959,652 - BBR_RS14190 0.86 -0.3 959,688 - BBR_RS14190 0.89 +0.9 959,735 - -0.3 959,758 - -0.3 959,908 - -0.4 959,929 - -0.1 959,932 + +0.2 959,964 - +0.3 960,142 + BBR_RS14200 0.15 +0.5 960,142 + BBR_RS14200 0.15 -0.4 960,150 - BBR_RS14200 0.17 +0.4 960,154 + BBR_RS14200 0.19 -0.2 960,154 + BBR_RS14200 0.19 -0.9 960,162 - BBR_RS14200 0.21 -1.2 960,162 - BBR_RS14200 0.21 +2.1 960,195 + BBR_RS14200 0.32 +0.8 960,205 + BBR_RS14200 0.36 -0.4 960,342 + BBR_RS14200 0.81 +0.5 960,350 - BBR_RS14200 0.84 +0.5 960,379 + +1.5 960,397 + +0.3 960,439 - -0.5 960,516 + +0.4 960,946 - BBR_RS14205 0.16 +0.2 961,366 + BBR_RS14205 0.30 -0.6 961,838 - BBR_RS14205 0.45 +0.8 962,195 - BBR_RS14205 0.57 -0.7 962,742 - BBR_RS14205 0.74 -0.1 962,794 + BBR_RS14205 0.76 -1.4 962,802 - BBR_RS14205 0.76 +1.3 962,827 + BBR_RS14205 0.77 -0.2 962,830 + BBR_RS14205 0.77 +0.2 962,836 + BBR_RS14205 0.77 -0.1 962,946 + BBR_RS14205 0.81 -0.4 963,245 + +0.2 963,275 - +0.3 963,383 + +0.6 963,391 + +0.0 963,391 + +1.5 963,392 + -0.2 963,399 - -0.6 963,414 + -0.1 963,422 - -0.3 963,456 - -0.8 963,483 - +0.2 963,495 + +0.5 963,516 + -0.0 963,555 + +1.1 963,709 - BBR_RS14210 0.11 -0.7 963,993 - BBR_RS14210 0.30 -0.7 964,075 + BBR_RS14210 0.35 -0.3 964,448 - BBR_RS14210 0.59 -0.6 964,526 - BBR_RS14210 0.64 -0.2
Or see this region's nucleotide sequence