Experiment: Recovery broth with Norfloxacin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13180 and BBR_RS13185 are separated by 48 nucleotides BBR_RS13185 and BBR_RS13190 overlap by 4 nucleotides BBR_RS13190 and BBR_RS13195 are separated by 210 nucleotides
BBR_RS13180: BBR_RS13180 - DNA primase, at 733,651 to 735,753
_RS13180
BBR_RS13185: BBR_RS13185 - PIN domain-containing protein, at 735,802 to 736,224
_RS13185
BBR_RS13190: BBR_RS13190 - hypothetical protein, at 736,221 to 736,451
_RS13190
BBR_RS13195: BBR_RS13195 - pyridoxal 5'-phosphate synthase lyase subunit PdxS, at 736,662 to 737,537
_RS13195
Position (kb) 735
736
737 Strain fitness (log2 ratio) -2
-1
0
1 at 735.794 kb on - strand at 735.915 kb on - strand, within BBR_RS13185 at 735.915 kb on - strand, within BBR_RS13185 at 736.005 kb on + strand, within BBR_RS13185 at 736.019 kb on + strand, within BBR_RS13185 at 736.038 kb on - strand, within BBR_RS13185 at 736.048 kb on - strand, within BBR_RS13185 at 736.099 kb on - strand, within BBR_RS13185 at 736.111 kb on + strand, within BBR_RS13185 at 736.135 kb on + strand, within BBR_RS13185 at 736.143 kb on + strand, within BBR_RS13185 at 736.143 kb on - strand, within BBR_RS13185 at 736.160 kb on + strand, within BBR_RS13185 at 736.164 kb on - strand, within BBR_RS13185 at 736.179 kb on + strand, within BBR_RS13185 at 736.225 kb on + strand at 736.587 kb on + strand at 736.587 kb on + strand at 736.587 kb on + strand at 736.595 kb on - strand at 736.598 kb on + strand at 736.600 kb on - strand at 736.632 kb on + strand at 736.638 kb on - strand at 736.640 kb on + strand at 736.647 kb on + strand at 736.788 kb on - strand, within BBR_RS13195 at 736.851 kb on + strand, within BBR_RS13195 at 736.856 kb on + strand, within BBR_RS13195 at 736.864 kb on - strand, within BBR_RS13195 at 737.001 kb on - strand, within BBR_RS13195 at 737.005 kb on - strand, within BBR_RS13195 at 737.049 kb on + strand, within BBR_RS13195 at 737.058 kb on - strand, within BBR_RS13195 at 737.062 kb on - strand, within BBR_RS13195 at 737.088 kb on + strand, within BBR_RS13195 at 737.137 kb on - strand, within BBR_RS13195 at 737.212 kb on + strand, within BBR_RS13195
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Norfloxacin 0.2 mM remove 735,794 - -1.6 735,915 - BBR_RS13185 0.27 -2.1 735,915 - BBR_RS13185 0.27 -1.3 736,005 + BBR_RS13185 0.48 +0.7 736,019 + BBR_RS13185 0.51 -0.1 736,038 - BBR_RS13185 0.56 -0.7 736,048 - BBR_RS13185 0.58 +0.4 736,099 - BBR_RS13185 0.70 -0.1 736,111 + BBR_RS13185 0.73 -0.5 736,135 + BBR_RS13185 0.79 -0.8 736,143 + BBR_RS13185 0.81 -0.7 736,143 - BBR_RS13185 0.81 -1.1 736,160 + BBR_RS13185 0.85 -1.5 736,164 - BBR_RS13185 0.86 +0.9 736,179 + BBR_RS13185 0.89 +0.2 736,225 + -0.1 736,587 + -0.9 736,587 + -0.3 736,587 + -0.1 736,595 - -0.9 736,598 + +1.1 736,600 - -0.1 736,632 + +1.1 736,638 - -0.1 736,640 + -0.8 736,647 + +0.9 736,788 - BBR_RS13195 0.14 +0.2 736,851 + BBR_RS13195 0.22 +0.2 736,856 + BBR_RS13195 0.22 -0.9 736,864 - BBR_RS13195 0.23 +0.6 737,001 - BBR_RS13195 0.39 -0.5 737,005 - BBR_RS13195 0.39 +0.2 737,049 + BBR_RS13195 0.44 +0.2 737,058 - BBR_RS13195 0.45 +0.4 737,062 - BBR_RS13195 0.46 +1.4 737,088 + BBR_RS13195 0.49 -0.2 737,137 - BBR_RS13195 0.54 +0.4 737,212 + BBR_RS13195 0.63 -0.2
Or see this region's nucleotide sequence