Experiment: Recovery broth with Norfloxacin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS20675 and BBR_RS20680 overlap by 16 nucleotides BBR_RS20680 and BBR_RS12235 are separated by 86 nucleotides BBR_RS12235 and BBR_RS12240 are separated by 129 nucleotides BBR_RS12240 and BBR_RS20685 overlap by 40 nucleotides BBR_RS20685 and BBR_RS12245 are separated by 40 nucleotides
BBR_RS20675: BBR_RS20675 - hypothetical protein, at 493,926 to 494,420
_RS20675
BBR_RS20680: BBR_RS20680 - hypothetical protein, at 494,405 to 494,641
_RS20680
BBR_RS12235: BBR_RS12235 - hypothetical protein, at 494,728 to 495,414
_RS12235
BBR_RS12240: BBR_RS12240 - 30S ribosomal protein S15, at 495,544 to 495,813
_RS12240
BBR_RS20685: BBR_RS20685 - hypothetical protein, at 495,774 to 496,049
_RS20685
BBR_RS12245: BBR_RS12245 - polyribonucleotide nucleotidyltransferase, at 496,090 to 498,801
_RS12245
Position (kb) 494
495
496 Strain fitness (log2 ratio) -2
-1
0
1 at 493.742 kb on + strand at 493.742 kb on + strand at 493.763 kb on + strand at 493.771 kb on - strand at 493.773 kb on + strand at 493.773 kb on + strand at 493.814 kb on + strand at 493.820 kb on - strand at 493.825 kb on - strand at 493.825 kb on - strand at 493.850 kb on + strand at 493.876 kb on + strand at 493.884 kb on - strand at 493.884 kb on - strand at 493.901 kb on + strand at 493.910 kb on + strand at 493.910 kb on + strand at 493.910 kb on + strand at 493.911 kb on - strand at 493.918 kb on - strand at 493.920 kb on + strand at 493.920 kb on - strand at 493.959 kb on - strand at 493.959 kb on - strand at 493.992 kb on + strand, within BBR_RS20675 at 494.001 kb on + strand, within BBR_RS20675 at 494.001 kb on + strand, within BBR_RS20675 at 494.021 kb on - strand, within BBR_RS20675 at 494.037 kb on - strand, within BBR_RS20675 at 494.114 kb on + strand, within BBR_RS20675 at 494.127 kb on - strand, within BBR_RS20675 at 494.129 kb on + strand, within BBR_RS20675 at 494.163 kb on - strand, within BBR_RS20675 at 494.163 kb on - strand, within BBR_RS20675 at 494.224 kb on - strand, within BBR_RS20675 at 494.248 kb on + strand, within BBR_RS20675 at 494.266 kb on + strand, within BBR_RS20675 at 494.390 kb on + strand at 494.390 kb on + strand at 494.390 kb on + strand at 494.390 kb on + strand at 494.395 kb on + strand at 494.395 kb on + strand at 494.423 kb on + strand at 494.440 kb on - strand, within BBR_RS20680 at 494.440 kb on - strand, within BBR_RS20680 at 494.470 kb on + strand, within BBR_RS20680 at 494.470 kb on + strand, within BBR_RS20680 at 494.470 kb on + strand, within BBR_RS20680 at 494.478 kb on - strand, within BBR_RS20680 at 494.482 kb on + strand, within BBR_RS20680 at 494.491 kb on - strand, within BBR_RS20680 at 494.588 kb on + strand, within BBR_RS20680 at 494.604 kb on + strand, within BBR_RS20680 at 494.618 kb on - strand at 494.626 kb on + strand at 494.626 kb on + strand at 494.628 kb on - strand at 494.634 kb on - strand at 494.636 kb on - strand at 494.636 kb on - strand at 494.657 kb on - strand at 494.672 kb on + strand at 494.676 kb on + strand at 494.684 kb on - strand at 494.716 kb on + strand at 494.726 kb on + strand at 494.731 kb on + strand at 494.733 kb on + strand at 494.755 kb on + strand at 494.787 kb on + strand at 494.787 kb on + strand at 494.871 kb on + strand, within BBR_RS12235 at 495.834 kb on + strand, within BBR_RS20685 at 495.840 kb on + strand, within BBR_RS20685 at 495.845 kb on + strand, within BBR_RS20685 at 495.845 kb on + strand, within BBR_RS20685 at 495.845 kb on + strand, within BBR_RS20685 at 495.892 kb on + strand, within BBR_RS20685 at 495.893 kb on + strand, within BBR_RS20685 at 495.893 kb on + strand, within BBR_RS20685 at 495.911 kb on + strand, within BBR_RS20685 at 495.927 kb on - strand, within BBR_RS20685 at 495.941 kb on - strand, within BBR_RS20685 at 495.988 kb on + strand, within BBR_RS20685 at 496.029 kb on + strand at 496.031 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Norfloxacin 0.2 mM remove 493,742 + -1.3 493,742 + -0.3 493,763 + -0.4 493,771 - +1.0 493,773 + +1.0 493,773 + -0.4 493,814 + +0.5 493,820 - -1.4 493,825 - -1.0 493,825 - -0.5 493,850 + +1.1 493,876 + +0.3 493,884 - -0.3 493,884 - +0.8 493,901 + +0.0 493,910 + -1.7 493,910 + +0.1 493,910 + +1.0 493,911 - +0.7 493,918 - -2.1 493,920 + +0.1 493,920 - +0.6 493,959 - +0.4 493,959 - -0.5 493,992 + BBR_RS20675 0.13 +0.3 494,001 + BBR_RS20675 0.15 +0.4 494,001 + BBR_RS20675 0.15 -0.8 494,021 - BBR_RS20675 0.19 -0.2 494,037 - BBR_RS20675 0.22 -0.3 494,114 + BBR_RS20675 0.38 +0.3 494,127 - BBR_RS20675 0.41 +1.3 494,129 + BBR_RS20675 0.41 +0.1 494,163 - BBR_RS20675 0.48 +0.0 494,163 - BBR_RS20675 0.48 -0.6 494,224 - BBR_RS20675 0.60 +0.0 494,248 + BBR_RS20675 0.65 -0.3 494,266 + BBR_RS20675 0.69 -1.2 494,390 + -0.5 494,390 + +0.7 494,390 + +0.2 494,390 + -0.1 494,395 + -0.7 494,395 + -0.9 494,423 + +1.5 494,440 - BBR_RS20680 0.15 -0.4 494,440 - BBR_RS20680 0.15 -0.4 494,470 + BBR_RS20680 0.27 +0.7 494,470 + BBR_RS20680 0.27 -0.1 494,470 + BBR_RS20680 0.27 +1.1 494,478 - BBR_RS20680 0.31 -1.0 494,482 + BBR_RS20680 0.32 +0.0 494,491 - BBR_RS20680 0.36 +1.2 494,588 + BBR_RS20680 0.77 +1.4 494,604 + BBR_RS20680 0.84 +0.5 494,618 - +0.1 494,626 + -1.4 494,626 + +0.2 494,628 - -0.2 494,634 - +0.4 494,636 - +1.0 494,636 - -0.5 494,657 - -0.6 494,672 + +1.0 494,676 + +0.9 494,684 - -1.0 494,716 + +0.8 494,726 + -0.3 494,731 + +0.8 494,733 + +0.2 494,755 + -0.4 494,787 + -0.1 494,787 + -0.0 494,871 + BBR_RS12235 0.21 -1.8 495,834 + BBR_RS20685 0.22 +0.2 495,840 + BBR_RS20685 0.24 -0.1 495,845 + BBR_RS20685 0.26 -1.3 495,845 + BBR_RS20685 0.26 +1.1 495,845 + BBR_RS20685 0.26 +0.2 495,892 + BBR_RS20685 0.43 +0.8 495,893 + BBR_RS20685 0.43 +0.7 495,893 + BBR_RS20685 0.43 -0.2 495,911 + BBR_RS20685 0.50 -1.2 495,927 - BBR_RS20685 0.55 +1.1 495,941 - BBR_RS20685 0.61 -1.1 495,988 + BBR_RS20685 0.78 -0.2 496,029 + -0.0 496,031 + +0.3
Or see this region's nucleotide sequence