Experiment: Recovery broth with Norfloxacin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS11200 and BBR_RS11205 are separated by 123 nucleotides BBR_RS11205 and BBR_RS11210 are separated by 257 nucleotides BBR_RS11210 and BBR_RS11215 are separated by 852 nucleotides
BBR_RS11200: BBR_RS11200 - LacI family transcriptional regulator, at 257,965 to 258,594
_RS11200
BBR_RS11205: BBR_RS11205 - HPr family phosphocarrier protein, at 258,718 to 258,978
_RS11205
BBR_RS11210: BBR_RS11210 - phosphoenolpyruvate--protein phosphotransferase, at 259,236 to 260,885
_RS11210
BBR_RS11215: BBR_RS11215 - hypothetical protein, at 261,738 to 262,544
_RS11215
Position (kb) 259
260
261 Strain fitness (log2 ratio) -2
-1
0
1
2 at 258.243 kb on - strand, within BBR_RS11200 at 258.261 kb on + strand, within BBR_RS11200 at 258.273 kb on + strand, within BBR_RS11200 at 258.294 kb on + strand, within BBR_RS11200 at 258.318 kb on + strand, within BBR_RS11200 at 258.318 kb on + strand, within BBR_RS11200 at 258.318 kb on + strand, within BBR_RS11200 at 258.318 kb on + strand, within BBR_RS11200 at 258.318 kb on + strand, within BBR_RS11200 at 258.318 kb on + strand, within BBR_RS11200 at 258.326 kb on - strand, within BBR_RS11200 at 258.326 kb on - strand, within BBR_RS11200 at 258.326 kb on - strand, within BBR_RS11200 at 258.326 kb on - strand, within BBR_RS11200 at 258.326 kb on - strand, within BBR_RS11200 at 258.359 kb on - strand, within BBR_RS11200 at 258.377 kb on - strand, within BBR_RS11200 at 258.391 kb on - strand, within BBR_RS11200 at 258.391 kb on - strand, within BBR_RS11200 at 258.391 kb on - strand, within BBR_RS11200 at 258.391 kb on - strand, within BBR_RS11200 at 258.397 kb on + strand, within BBR_RS11200 at 258.403 kb on - strand, within BBR_RS11200 at 258.403 kb on - strand, within BBR_RS11200 at 258.403 kb on - strand, within BBR_RS11200 at 258.403 kb on - strand, within BBR_RS11200 at 258.403 kb on - strand, within BBR_RS11200 at 258.403 kb on - strand, within BBR_RS11200 at 258.432 kb on + strand, within BBR_RS11200 at 258.432 kb on + strand, within BBR_RS11200 at 258.432 kb on + strand, within BBR_RS11200 at 258.440 kb on - strand, within BBR_RS11200 at 258.465 kb on + strand, within BBR_RS11200 at 258.503 kb on + strand, within BBR_RS11200 at 258.508 kb on + strand, within BBR_RS11200 at 258.516 kb on - strand, within BBR_RS11200 at 258.516 kb on - strand, within BBR_RS11200 at 258.540 kb on + strand at 258.553 kb on + strand at 258.571 kb on - strand at 258.571 kb on - strand at 258.598 kb on - strand at 258.653 kb on + strand at 258.745 kb on - strand, within BBR_RS11205 at 258.797 kb on + strand, within BBR_RS11205 at 258.797 kb on + strand, within BBR_RS11205 at 258.805 kb on - strand, within BBR_RS11205 at 258.805 kb on - strand, within BBR_RS11205 at 258.912 kb on - strand, within BBR_RS11205 at 258.961 kb on - strand at 258.983 kb on - strand at 258.983 kb on - strand at 258.997 kb on - strand at 259.045 kb on - strand at 259.045 kb on - strand at 259.045 kb on - strand at 259.046 kb on + strand at 259.052 kb on - strand at 259.087 kb on + strand at 259.101 kb on - strand at 259.162 kb on + strand at 259.170 kb on + strand at 259.170 kb on + strand at 259.183 kb on + strand at 259.211 kb on - strand at 259.213 kb on - strand at 259.214 kb on - strand at 259.214 kb on - strand at 259.231 kb on + strand at 259.347 kb on - strand at 259.481 kb on - strand, within BBR_RS11210 at 259.523 kb on + strand, within BBR_RS11210 at 259.567 kb on - strand, within BBR_RS11210 at 259.575 kb on + strand, within BBR_RS11210 at 259.644 kb on - strand, within BBR_RS11210 at 259.644 kb on - strand, within BBR_RS11210 at 259.644 kb on - strand, within BBR_RS11210 at 259.657 kb on - strand, within BBR_RS11210 at 259.667 kb on - strand, within BBR_RS11210 at 259.669 kb on - strand, within BBR_RS11210 at 259.767 kb on + strand, within BBR_RS11210 at 259.768 kb on - strand, within BBR_RS11210 at 259.768 kb on - strand, within BBR_RS11210 at 259.869 kb on + strand, within BBR_RS11210 at 259.874 kb on + strand, within BBR_RS11210 at 259.874 kb on + strand, within BBR_RS11210 at 259.890 kb on - strand, within BBR_RS11210 at 259.897 kb on - strand, within BBR_RS11210 at 259.983 kb on + strand, within BBR_RS11210 at 259.985 kb on - strand, within BBR_RS11210 at 260.018 kb on + strand, within BBR_RS11210 at 260.047 kb on - strand, within BBR_RS11210 at 260.076 kb on + strand, within BBR_RS11210 at 260.084 kb on - strand, within BBR_RS11210 at 260.084 kb on - strand, within BBR_RS11210 at 260.105 kb on - strand, within BBR_RS11210 at 260.106 kb on - strand, within BBR_RS11210 at 260.111 kb on - strand, within BBR_RS11210 at 260.137 kb on + strand, within BBR_RS11210 at 260.165 kb on + strand, within BBR_RS11210 at 260.171 kb on + strand, within BBR_RS11210 at 260.171 kb on + strand, within BBR_RS11210 at 260.171 kb on + strand, within BBR_RS11210 at 260.171 kb on + strand, within BBR_RS11210 at 260.171 kb on + strand, within BBR_RS11210 at 260.171 kb on + strand, within BBR_RS11210 at 260.179 kb on - strand, within BBR_RS11210 at 260.192 kb on - strand, within BBR_RS11210 at 260.194 kb on - strand, within BBR_RS11210 at 260.211 kb on + strand, within BBR_RS11210 at 260.295 kb on - strand, within BBR_RS11210 at 260.301 kb on + strand, within BBR_RS11210 at 260.311 kb on + strand, within BBR_RS11210 at 260.311 kb on + strand, within BBR_RS11210 at 260.353 kb on - strand, within BBR_RS11210 at 260.365 kb on - strand, within BBR_RS11210 at 260.370 kb on + strand, within BBR_RS11210 at 260.375 kb on + strand, within BBR_RS11210 at 260.409 kb on - strand, within BBR_RS11210 at 260.409 kb on - strand, within BBR_RS11210 at 260.410 kb on - strand, within BBR_RS11210 at 260.473 kb on - strand, within BBR_RS11210 at 260.551 kb on + strand, within BBR_RS11210 at 260.612 kb on + strand, within BBR_RS11210 at 260.620 kb on - strand, within BBR_RS11210 at 260.655 kb on - strand, within BBR_RS11210 at 260.691 kb on - strand, within BBR_RS11210 at 260.702 kb on + strand, within BBR_RS11210 at 260.765 kb on + strand at 260.834 kb on + strand at 260.855 kb on + strand at 260.862 kb on + strand at 260.864 kb on + strand at 260.864 kb on + strand at 260.864 kb on + strand at 260.909 kb on - strand at 260.929 kb on + strand at 260.929 kb on + strand at 260.989 kb on + strand at 261.054 kb on - strand at 261.093 kb on - strand at 261.100 kb on + strand at 261.101 kb on - strand at 261.101 kb on - strand at 261.101 kb on - strand at 261.110 kb on - strand at 261.110 kb on - strand at 261.129 kb on - strand at 261.129 kb on - strand at 261.159 kb on - strand at 261.180 kb on - strand at 261.180 kb on - strand at 261.182 kb on + strand at 261.190 kb on - strand at 261.220 kb on + strand at 261.220 kb on + strand at 261.250 kb on + strand at 261.255 kb on + strand at 261.264 kb on - strand at 261.264 kb on - strand at 261.267 kb on + strand at 261.270 kb on - strand at 261.270 kb on - strand at 261.270 kb on - strand at 261.292 kb on + strand at 261.292 kb on + strand at 261.298 kb on + strand at 261.305 kb on + strand at 261.308 kb on + strand at 261.308 kb on + strand at 261.316 kb on - strand at 261.323 kb on - strand at 261.323 kb on - strand at 261.346 kb on + strand at 261.354 kb on - strand at 261.354 kb on - strand at 261.354 kb on - strand at 261.354 kb on - strand at 261.364 kb on - strand at 261.364 kb on - strand at 261.381 kb on - strand at 261.388 kb on - strand at 261.394 kb on - strand at 261.394 kb on - strand at 261.427 kb on + strand at 261.427 kb on + strand at 261.430 kb on - strand at 261.431 kb on - strand at 261.431 kb on - strand at 261.433 kb on + strand at 261.435 kb on - strand at 261.439 kb on + strand at 261.439 kb on + strand at 261.439 kb on + strand at 261.439 kb on + strand at 261.439 kb on + strand at 261.439 kb on + strand at 261.440 kb on + strand at 261.440 kb on + strand at 261.440 kb on + strand at 261.447 kb on - strand at 261.447 kb on - strand at 261.448 kb on - strand at 261.448 kb on - strand at 261.448 kb on - strand at 261.453 kb on + strand at 261.461 kb on - strand at 261.466 kb on - strand at 261.523 kb on - strand at 261.551 kb on - strand at 261.564 kb on + strand at 261.564 kb on + strand at 261.566 kb on + strand at 261.569 kb on + strand at 261.572 kb on - strand at 261.572 kb on - strand at 261.579 kb on - strand at 261.582 kb on - strand at 261.585 kb on - strand at 261.592 kb on + strand at 261.592 kb on + strand at 261.592 kb on + strand at 261.592 kb on + strand at 261.592 kb on + strand at 261.600 kb on - strand at 261.607 kb on - strand at 261.607 kb on - strand at 261.612 kb on + strand at 261.624 kb on - strand at 261.677 kb on + strand at 261.695 kb on + strand at 261.695 kb on + strand at 261.695 kb on + strand at 261.695 kb on + strand at 261.710 kb on - strand at 261.753 kb on + strand at 261.760 kb on + strand at 261.767 kb on - strand at 261.770 kb on + strand at 261.777 kb on + strand at 261.777 kb on - strand at 261.777 kb on - strand at 261.777 kb on - strand at 261.813 kb on - strand at 261.813 kb on - strand at 261.820 kb on + strand, within BBR_RS11215 at 261.825 kb on + strand, within BBR_RS11215 at 261.825 kb on + strand, within BBR_RS11215 at 261.825 kb on + strand, within BBR_RS11215 at 261.825 kb on + strand, within BBR_RS11215 at 261.825 kb on + strand, within BBR_RS11215 at 261.825 kb on + strand, within BBR_RS11215 at 261.825 kb on + strand, within BBR_RS11215 at 261.833 kb on - strand, within BBR_RS11215 at 261.833 kb on - strand, within BBR_RS11215 at 261.833 kb on - strand, within BBR_RS11215 at 261.833 kb on - strand, within BBR_RS11215 at 261.833 kb on - strand, within BBR_RS11215 at 261.833 kb on - strand, within BBR_RS11215 at 261.833 kb on - strand, within BBR_RS11215 at 261.836 kb on - strand, within BBR_RS11215 at 261.836 kb on - strand, within BBR_RS11215 at 261.844 kb on - strand, within BBR_RS11215 at 261.846 kb on + strand, within BBR_RS11215 at 261.851 kb on + strand, within BBR_RS11215 at 261.851 kb on + strand, within BBR_RS11215 at 261.854 kb on - strand, within BBR_RS11215 at 261.854 kb on - strand, within BBR_RS11215 at 261.858 kb on + strand, within BBR_RS11215 at 261.859 kb on - strand, within BBR_RS11215 at 261.859 kb on - strand, within BBR_RS11215 at 261.859 kb on - strand, within BBR_RS11215 at 261.859 kb on - strand, within BBR_RS11215 at 261.859 kb on - strand, within BBR_RS11215 at 261.859 kb on - strand, within BBR_RS11215 at 261.871 kb on - strand, within BBR_RS11215 at 261.876 kb on - strand, within BBR_RS11215
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Norfloxacin 0.2 mM remove 258,243 - BBR_RS11200 0.44 +1.3 258,261 + BBR_RS11200 0.47 +0.3 258,273 + BBR_RS11200 0.49 +1.2 258,294 + BBR_RS11200 0.52 +0.9 258,318 + BBR_RS11200 0.56 -0.6 258,318 + BBR_RS11200 0.56 -0.1 258,318 + BBR_RS11200 0.56 -0.2 258,318 + BBR_RS11200 0.56 -0.4 258,318 + BBR_RS11200 0.56 +0.4 258,318 + BBR_RS11200 0.56 -0.7 258,326 - BBR_RS11200 0.57 +0.7 258,326 - BBR_RS11200 0.57 +0.5 258,326 - BBR_RS11200 0.57 -0.4 258,326 - BBR_RS11200 0.57 -1.2 258,326 - BBR_RS11200 0.57 +0.2 258,359 - BBR_RS11200 0.63 +0.6 258,377 - BBR_RS11200 0.65 -1.3 258,391 - BBR_RS11200 0.68 -0.2 258,391 - BBR_RS11200 0.68 -2.5 258,391 - BBR_RS11200 0.68 -1.6 258,391 - BBR_RS11200 0.68 +0.2 258,397 + BBR_RS11200 0.69 -0.8 258,403 - BBR_RS11200 0.70 -0.7 258,403 - BBR_RS11200 0.70 -2.5 258,403 - BBR_RS11200 0.70 -0.5 258,403 - BBR_RS11200 0.70 +0.1 258,403 - BBR_RS11200 0.70 +1.1 258,403 - BBR_RS11200 0.70 -0.3 258,432 + BBR_RS11200 0.74 +1.2 258,432 + BBR_RS11200 0.74 -0.4 258,432 + BBR_RS11200 0.74 -1.0 258,440 - BBR_RS11200 0.75 -0.4 258,465 + BBR_RS11200 0.79 +0.4 258,503 + BBR_RS11200 0.85 -0.5 258,508 + BBR_RS11200 0.86 -0.3 258,516 - BBR_RS11200 0.87 -0.1 258,516 - BBR_RS11200 0.87 -1.4 258,540 + -0.4 258,553 + -1.5 258,571 - -0.5 258,571 - -0.9 258,598 - +0.7 258,653 + +1.6 258,745 - BBR_RS11205 0.10 -0.8 258,797 + BBR_RS11205 0.30 +0.1 258,797 + BBR_RS11205 0.30 -0.6 258,805 - BBR_RS11205 0.33 -2.3 258,805 - BBR_RS11205 0.33 -0.4 258,912 - BBR_RS11205 0.74 -0.5 258,961 - -1.2 258,983 - -1.0 258,983 - -1.3 258,997 - -1.6 259,045 - -0.1 259,045 - -0.6 259,045 - -0.7 259,046 + +0.4 259,052 - -1.4 259,087 + +0.0 259,101 - +0.6 259,162 + -2.5 259,170 + +0.4 259,170 + -0.1 259,183 + -0.3 259,211 - +0.2 259,213 - -0.2 259,214 - -0.9 259,214 - +0.5 259,231 + +1.0 259,347 - +1.9 259,481 - BBR_RS11210 0.15 -0.5 259,523 + BBR_RS11210 0.17 -1.3 259,567 - BBR_RS11210 0.20 +0.1 259,575 + BBR_RS11210 0.21 -1.4 259,644 - BBR_RS11210 0.25 -0.5 259,644 - BBR_RS11210 0.25 -1.9 259,644 - BBR_RS11210 0.25 -1.4 259,657 - BBR_RS11210 0.26 -0.3 259,667 - BBR_RS11210 0.26 -1.2 259,669 - BBR_RS11210 0.26 -0.8 259,767 + BBR_RS11210 0.32 +0.4 259,768 - BBR_RS11210 0.32 +1.7 259,768 - BBR_RS11210 0.32 -0.9 259,869 + BBR_RS11210 0.38 -2.4 259,874 + BBR_RS11210 0.39 +0.0 259,874 + BBR_RS11210 0.39 -1.0 259,890 - BBR_RS11210 0.40 +0.1 259,897 - BBR_RS11210 0.40 +0.8 259,983 + BBR_RS11210 0.45 -1.0 259,985 - BBR_RS11210 0.45 -2.0 260,018 + BBR_RS11210 0.47 -1.6 260,047 - BBR_RS11210 0.49 -1.1 260,076 + BBR_RS11210 0.51 -0.3 260,084 - BBR_RS11210 0.51 -0.6 260,084 - BBR_RS11210 0.51 +0.3 260,105 - BBR_RS11210 0.53 -0.3 260,106 - BBR_RS11210 0.53 -0.7 260,111 - BBR_RS11210 0.53 +0.3 260,137 + BBR_RS11210 0.55 -0.8 260,165 + BBR_RS11210 0.56 -0.4 260,171 + BBR_RS11210 0.57 -0.5 260,171 + BBR_RS11210 0.57 -2.0 260,171 + BBR_RS11210 0.57 -0.4 260,171 + BBR_RS11210 0.57 +0.6 260,171 + BBR_RS11210 0.57 -0.9 260,171 + BBR_RS11210 0.57 -1.2 260,179 - BBR_RS11210 0.57 +0.2 260,192 - BBR_RS11210 0.58 +0.2 260,194 - BBR_RS11210 0.58 -1.1 260,211 + BBR_RS11210 0.59 -0.6 260,295 - BBR_RS11210 0.64 +0.4 260,301 + BBR_RS11210 0.65 -1.2 260,311 + BBR_RS11210 0.65 -0.0 260,311 + BBR_RS11210 0.65 -1.5 260,353 - BBR_RS11210 0.68 -0.1 260,365 - BBR_RS11210 0.68 -0.8 260,370 + BBR_RS11210 0.69 -0.4 260,375 + BBR_RS11210 0.69 -0.9 260,409 - BBR_RS11210 0.71 -0.9 260,409 - BBR_RS11210 0.71 -0.9 260,410 - BBR_RS11210 0.71 +2.4 260,473 - BBR_RS11210 0.75 -0.9 260,551 + BBR_RS11210 0.80 -1.1 260,612 + BBR_RS11210 0.83 -1.4 260,620 - BBR_RS11210 0.84 -0.7 260,655 - BBR_RS11210 0.86 -1.1 260,691 - BBR_RS11210 0.88 -1.1 260,702 + BBR_RS11210 0.89 -0.5 260,765 + -1.9 260,834 + -1.9 260,855 + +0.1 260,862 + -0.5 260,864 + -0.1 260,864 + -0.9 260,864 + +0.2 260,909 - -0.8 260,929 + -1.3 260,929 + -0.2 260,989 + +0.9 261,054 - +1.1 261,093 - +0.3 261,100 + -0.4 261,101 - -1.1 261,101 - -0.1 261,101 - +0.1 261,110 - -1.4 261,110 - +1.7 261,129 - -0.1 261,129 - +1.6 261,159 - -0.1 261,180 - +0.0 261,180 - -0.5 261,182 + -0.5 261,190 - +0.3 261,220 + +0.1 261,220 + +1.1 261,250 + -0.5 261,255 + +0.8 261,264 - -0.8 261,264 - -0.3 261,267 + -0.2 261,270 - +0.2 261,270 - +0.6 261,270 - -0.2 261,292 + -0.6 261,292 + -0.3 261,298 + -0.3 261,305 + -0.4 261,308 + +0.1 261,308 + -0.6 261,316 - -0.3 261,323 - +0.8 261,323 - +0.4 261,346 + -0.8 261,354 - +0.0 261,354 - +0.6 261,354 - -1.1 261,354 - -1.0 261,364 - +0.7 261,364 - +0.5 261,381 - -0.4 261,388 - +0.0 261,394 - +0.1 261,394 - -0.2 261,427 + -0.5 261,427 + +0.4 261,430 - -0.8 261,431 - +0.9 261,431 - +0.8 261,433 + +0.5 261,435 - -0.6 261,439 + -0.0 261,439 + +0.2 261,439 + +1.4 261,439 + +0.4 261,439 + -0.3 261,439 + +0.5 261,440 + +0.6 261,440 + -0.3 261,440 + -0.7 261,447 - -0.1 261,447 - +0.1 261,448 - -0.1 261,448 - -0.3 261,448 - -0.0 261,453 + -0.5 261,461 - -1.0 261,466 - -0.8 261,523 - -0.2 261,551 - +0.2 261,564 + -2.3 261,564 + -0.2 261,566 + +0.7 261,569 + +0.0 261,572 - -1.3 261,572 - +0.1 261,579 - +0.9 261,582 - +1.0 261,585 - -1.5 261,592 + +0.5 261,592 + -0.5 261,592 + -0.2 261,592 + +0.6 261,592 + -0.9 261,600 - -0.5 261,607 - -0.0 261,607 - -0.8 261,612 + +0.6 261,624 - +0.2 261,677 + -0.1 261,695 + +0.0 261,695 + +0.7 261,695 + -1.3 261,695 + +0.2 261,710 - -0.9 261,753 + -0.3 261,760 + +0.6 261,767 - +0.8 261,770 + -0.7 261,777 + -0.3 261,777 - -0.3 261,777 - +0.5 261,777 - +0.5 261,813 - +0.7 261,813 - -0.1 261,820 + BBR_RS11215 0.10 -0.3 261,825 + BBR_RS11215 0.11 -0.4 261,825 + BBR_RS11215 0.11 -0.2 261,825 + BBR_RS11215 0.11 -0.9 261,825 + BBR_RS11215 0.11 +1.6 261,825 + BBR_RS11215 0.11 -0.6 261,825 + BBR_RS11215 0.11 -0.1 261,825 + BBR_RS11215 0.11 +1.1 261,833 - BBR_RS11215 0.12 +0.3 261,833 - BBR_RS11215 0.12 +0.9 261,833 - BBR_RS11215 0.12 -0.7 261,833 - BBR_RS11215 0.12 -0.0 261,833 - BBR_RS11215 0.12 +0.3 261,833 - BBR_RS11215 0.12 +0.1 261,833 - BBR_RS11215 0.12 +0.9 261,836 - BBR_RS11215 0.12 +0.2 261,836 - BBR_RS11215 0.12 +0.1 261,844 - BBR_RS11215 0.13 -1.2 261,846 + BBR_RS11215 0.13 -1.4 261,851 + BBR_RS11215 0.14 -0.7 261,851 + BBR_RS11215 0.14 +0.1 261,854 - BBR_RS11215 0.14 -0.7 261,854 - BBR_RS11215 0.14 -0.5 261,858 + BBR_RS11215 0.15 -0.0 261,859 - BBR_RS11215 0.15 -0.5 261,859 - BBR_RS11215 0.15 +0.2 261,859 - BBR_RS11215 0.15 +0.1 261,859 - BBR_RS11215 0.15 -1.3 261,859 - BBR_RS11215 0.15 +1.1 261,859 - BBR_RS11215 0.15 +0.5 261,871 - BBR_RS11215 0.16 +1.2 261,876 - BBR_RS11215 0.17 +0.5
Or see this region's nucleotide sequence