Strain Fitness in Paraburkholderia graminis OAS925 around ABIE53_001315

Experiment: RCH2_defined_glucose_5mM with Adenine 2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABIE53_001314 and ABIE53_001315 are separated by 471 nucleotidesABIE53_001315 and ABIE53_001316 overlap by 1 nucleotides ABIE53_001314: ABIE53_001314 - phosphoenolpyruvate carboxylase, at 1,468,432 to 1,471,659 _001314 ABIE53_001315: ABIE53_001315 - hydroxymethylbilane synthase, at 1,472,131 to 1,473,156 _001315 ABIE53_001316: ABIE53_001316 - uroporphyrinogen III methyltransferase/synthase, at 1,473,156 to 1,475,189 _001316 Position (kb) 1472 1473 1474Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1471.196 kb on + strand, within ABIE53_001314at 1471.691 kb on + strandat 1471.691 kb on + strandat 1471.707 kb on - strandat 1471.758 kb on + strandat 1471.759 kb on - strandat 1471.813 kb on - strandat 1471.927 kb on + strandat 1472.021 kb on - strandat 1472.021 kb on - strandat 1472.087 kb on + strandat 1472.087 kb on + strandat 1472.100 kb on + strandat 1472.100 kb on + strandat 1472.100 kb on + strandat 1472.100 kb on + strandat 1472.100 kb on + strandat 1472.129 kb on + strandat 1472.129 kb on + strandat 1473.196 kb on + strandat 1473.962 kb on + strand, within ABIE53_001316at 1474.024 kb on + strand, within ABIE53_001316at 1474.024 kb on + strand, within ABIE53_001316at 1474.024 kb on + strand, within ABIE53_001316at 1474.024 kb on + strand, within ABIE53_001316at 1474.025 kb on - strand, within ABIE53_001316

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose_5mM with Adenine 2 mM
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1,471,196 + ABIE53_001314 0.86 -2.5
1,471,691 + -1.2
1,471,691 + -0.0
1,471,707 - -2.3
1,471,758 + -0.0
1,471,759 - -0.7
1,471,813 - +1.5
1,471,927 + -2.0
1,472,021 - -0.0
1,472,021 - +0.7
1,472,087 + -1.1
1,472,087 + -0.4
1,472,100 + -0.3
1,472,100 + -1.0
1,472,100 + +1.0
1,472,100 + -1.5
1,472,100 + -2.7
1,472,129 + -2.6
1,472,129 + +1.0
1,473,196 + -0.4
1,473,962 + ABIE53_001316 0.40 +0.4
1,474,024 + ABIE53_001316 0.43 +0.1
1,474,024 + ABIE53_001316 0.43 +1.3
1,474,024 + ABIE53_001316 0.43 -0.7
1,474,024 + ABIE53_001316 0.43 -1.0
1,474,025 - ABIE53_001316 0.43 -4.3

Or see this region's nucleotide sequence