Experiment: Recovery broth with Levofloxacin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12555 and BBR_RS12560 are separated by 391 nucleotides BBR_RS12560 and BBR_RS12565 are separated by 103 nucleotides BBR_RS12565 and BBR_RS12570 are separated by 2 nucleotides
BBR_RS12555: BBR_RS12555 - protein-tyrosine-phosphatase, at 575,152 to 575,757
_RS12555
BBR_RS12560: BBR_RS12560 - transposase, at 576,149 to 576,799
_RS12560
BBR_RS12565: BBR_RS12565 - ATPase AAA, at 576,903 to 577,688
_RS12565
BBR_RS12570: BBR_RS12570 - IS21 family transposase, at 577,691 to 579,172
_RS12570
Position (kb) 576
577 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 575.194 kb on + strand at 575.265 kb on + strand, within BBR_RS12555 at 575.265 kb on + strand, within BBR_RS12555 at 575.271 kb on + strand, within BBR_RS12555 at 575.287 kb on + strand, within BBR_RS12555 at 575.287 kb on + strand, within BBR_RS12555 at 575.292 kb on + strand, within BBR_RS12555 at 575.337 kb on + strand, within BBR_RS12555 at 575.349 kb on + strand, within BBR_RS12555 at 575.356 kb on + strand, within BBR_RS12555 at 575.430 kb on + strand, within BBR_RS12555 at 575.430 kb on + strand, within BBR_RS12555 at 575.503 kb on + strand, within BBR_RS12555 at 575.508 kb on + strand, within BBR_RS12555 at 575.523 kb on - strand, within BBR_RS12555 at 575.553 kb on - strand, within BBR_RS12555 at 575.657 kb on - strand, within BBR_RS12555 at 575.672 kb on - strand, within BBR_RS12555 at 575.686 kb on + strand, within BBR_RS12555 at 575.750 kb on - strand at 575.760 kb on - strand at 575.776 kb on + strand at 575.805 kb on + strand at 575.805 kb on + strand at 575.949 kb on - strand at 576.027 kb on + strand at 576.049 kb on + strand at 576.064 kb on + strand at 576.064 kb on - strand at 576.072 kb on - strand at 576.083 kb on + strand at 576.091 kb on - strand at 576.100 kb on + strand at 576.121 kb on + strand at 576.182 kb on + strand at 576.192 kb on + strand at 576.208 kb on - strand at 576.210 kb on - strand at 576.210 kb on - strand at 576.210 kb on - strand at 576.240 kb on - strand, within BBR_RS12560 at 576.324 kb on - strand, within BBR_RS12560 at 576.415 kb on - strand, within BBR_RS12560 at 576.415 kb on - strand, within BBR_RS12560 at 576.415 kb on - strand, within BBR_RS12560 at 576.425 kb on - strand, within BBR_RS12560 at 576.455 kb on + strand, within BBR_RS12560 at 576.466 kb on + strand, within BBR_RS12560 at 576.555 kb on + strand, within BBR_RS12560 at 576.555 kb on + strand, within BBR_RS12560 at 576.577 kb on + strand, within BBR_RS12560 at 576.596 kb on - strand, within BBR_RS12560 at 576.649 kb on - strand, within BBR_RS12560 at 576.676 kb on + strand, within BBR_RS12560 at 576.728 kb on - strand, within BBR_RS12560 at 576.754 kb on - strand at 576.764 kb on + strand at 576.765 kb on - strand at 576.765 kb on - strand at 576.786 kb on + strand at 576.794 kb on - strand at 576.794 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Levofloxacin 0.2 mM remove 575,194 + -1.2 575,265 + BBR_RS12555 0.19 -2.2 575,265 + BBR_RS12555 0.19 +0.4 575,271 + BBR_RS12555 0.20 -2.4 575,287 + BBR_RS12555 0.22 -0.9 575,287 + BBR_RS12555 0.22 -1.9 575,292 + BBR_RS12555 0.23 -1.2 575,337 + BBR_RS12555 0.31 -1.6 575,349 + BBR_RS12555 0.33 -0.3 575,356 + BBR_RS12555 0.34 -2.6 575,430 + BBR_RS12555 0.46 -2.6 575,430 + BBR_RS12555 0.46 -0.1 575,503 + BBR_RS12555 0.58 +0.1 575,508 + BBR_RS12555 0.59 -1.5 575,523 - BBR_RS12555 0.61 +0.4 575,553 - BBR_RS12555 0.66 -2.4 575,657 - BBR_RS12555 0.83 -2.9 575,672 - BBR_RS12555 0.86 +2.8 575,686 + BBR_RS12555 0.88 -0.7 575,750 - -1.0 575,760 - +1.1 575,776 + -0.6 575,805 + -0.3 575,805 + +2.8 575,949 - +1.5 576,027 + +1.4 576,049 + +0.2 576,064 + +0.7 576,064 - -1.7 576,072 - -0.7 576,083 + +0.9 576,091 - -0.1 576,100 + -1.0 576,121 + +0.7 576,182 + -0.6 576,192 + -0.2 576,208 - -1.1 576,210 - +0.0 576,210 - +0.6 576,210 - -0.9 576,240 - BBR_RS12560 0.14 -3.2 576,324 - BBR_RS12560 0.27 +0.1 576,415 - BBR_RS12560 0.41 -2.9 576,415 - BBR_RS12560 0.41 -0.8 576,415 - BBR_RS12560 0.41 -2.5 576,425 - BBR_RS12560 0.42 -1.1 576,455 + BBR_RS12560 0.47 +0.1 576,466 + BBR_RS12560 0.49 -0.1 576,555 + BBR_RS12560 0.62 -3.4 576,555 + BBR_RS12560 0.62 -0.9 576,577 + BBR_RS12560 0.66 -2.5 576,596 - BBR_RS12560 0.69 -0.3 576,649 - BBR_RS12560 0.77 -0.6 576,676 + BBR_RS12560 0.81 +0.7 576,728 - BBR_RS12560 0.89 -0.8 576,754 - +1.9 576,764 + -0.6 576,765 - -1.0 576,765 - -2.3 576,786 + -0.1 576,794 - -0.6 576,794 - -0.5
Or see this region's nucleotide sequence