Experiment: MoYLS4 with Nickel Chloride 0.75mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mcpD and cheW-1 are separated by 45 nucleotides cheW-1 and LysE are separated by 359 nucleotides LysE and iciA are separated by 97 nucleotides
DVU0591: mcpD - methyl-accepting chemotaxis protein (TIGR), at 659,306 to 661,264
mcpD
DVU0592: cheW-1 - chemotaxis protein CheW (TIGR), at 661,310 to 661,831
cheW-1
DVU0593: LysE - L-lysine exporter, putative (TIGR), at 662,191 to 662,811
LysE
DVU0594: iciA - chromosome initiation inhibitor (TIGR), at 662,909 to 663,811
iciA
Position (kb) 662
663 Strain fitness (log2 ratio) -2
-1
0
1
2 at 661.235 kb on + strand at 661.258 kb on + strand at 661.258 kb on + strand at 661.258 kb on + strand at 661.258 kb on + strand at 661.266 kb on - strand at 661.266 kb on - strand at 661.278 kb on + strand at 661.283 kb on + strand at 661.327 kb on + strand at 661.343 kb on - strand at 661.343 kb on - strand at 661.355 kb on - strand at 661.364 kb on - strand, within cheW-1 at 661.411 kb on - strand, within cheW-1 at 661.433 kb on - strand, within cheW-1 at 661.438 kb on - strand, within cheW-1 at 661.461 kb on + strand, within cheW-1 at 661.475 kb on + strand, within cheW-1 at 661.511 kb on + strand, within cheW-1 at 661.704 kb on + strand, within cheW-1 at 661.750 kb on + strand, within cheW-1 at 661.750 kb on + strand, within cheW-1 at 661.901 kb on - strand at 661.912 kb on - strand at 661.961 kb on + strand at 661.980 kb on + strand at 662.010 kb on + strand at 662.079 kb on + strand at 662.079 kb on + strand at 662.079 kb on + strand at 662.079 kb on + strand at 662.079 kb on + strand at 662.087 kb on - strand at 662.087 kb on - strand at 662.241 kb on + strand at 662.241 kb on + strand at 662.241 kb on - strand at 662.251 kb on + strand at 662.265 kb on - strand, within LysE at 662.306 kb on - strand, within LysE at 662.407 kb on - strand, within LysE at 662.452 kb on - strand, within LysE at 662.468 kb on + strand, within LysE at 662.491 kb on - strand, within LysE at 662.545 kb on - strand, within LysE at 662.641 kb on - strand, within LysE at 662.728 kb on - strand, within LysE at 662.780 kb on - strand at 662.787 kb on + strand at 662.795 kb on - strand at 662.837 kb on + strand at 662.842 kb on + strand at 662.916 kb on + strand at 662.924 kb on - strand at 662.926 kb on + strand at 662.926 kb on + strand at 662.989 kb on - strand at 663.026 kb on + strand, within iciA at 663.034 kb on - strand, within iciA at 663.036 kb on + strand, within iciA at 663.061 kb on + strand, within iciA at 663.069 kb on - strand, within iciA at 663.074 kb on - strand, within iciA at 663.076 kb on + strand, within iciA at 663.111 kb on + strand, within iciA at 663.117 kb on + strand, within iciA at 663.119 kb on - strand, within iciA at 663.119 kb on - strand, within iciA at 663.139 kb on + strand, within iciA at 663.147 kb on - strand, within iciA at 663.173 kb on - strand, within iciA at 663.196 kb on + strand, within iciA at 663.370 kb on + strand, within iciA at 663.378 kb on - strand, within iciA at 663.383 kb on - strand, within iciA at 663.383 kb on - strand, within iciA at 663.383 kb on - strand, within iciA at 663.407 kb on - strand, within iciA at 663.454 kb on - strand, within iciA at 663.626 kb on - strand, within iciA at 663.714 kb on - strand, within iciA at 663.790 kb on - strand at 663.790 kb on - strand at 663.790 kb on - strand at 663.808 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Nickel Chloride 0.75mM remove 661,235 + -1.6 661,258 + +0.3 661,258 + +0.0 661,258 + -0.6 661,258 + -0.2 661,266 - +0.2 661,266 - -0.0 661,278 + -0.2 661,283 + -0.2 661,327 + +0.3 661,343 - -0.1 661,343 - -0.2 661,355 - +0.2 661,364 - cheW-1 DVU0592 0.10 -1.3 661,411 - cheW-1 DVU0592 0.19 +0.7 661,433 - cheW-1 DVU0592 0.24 +0.2 661,438 - cheW-1 DVU0592 0.25 +0.5 661,461 + cheW-1 DVU0592 0.29 -0.3 661,475 + cheW-1 DVU0592 0.32 -0.3 661,511 + cheW-1 DVU0592 0.39 +0.2 661,704 + cheW-1 DVU0592 0.75 -0.9 661,750 + cheW-1 DVU0592 0.84 -0.5 661,750 + cheW-1 DVU0592 0.84 -1.3 661,901 - +2.3 661,912 - +0.4 661,961 + +0.4 661,980 + -0.8 662,010 + +0.0 662,079 + -1.5 662,079 + -1.3 662,079 + -1.4 662,079 + -0.1 662,079 + +0.3 662,087 - +0.3 662,087 - +0.7 662,241 + +0.2 662,241 + -0.1 662,241 - +0.3 662,251 + +1.3 662,265 - LysE DVU0593 0.12 -0.2 662,306 - LysE DVU0593 0.19 -0.0 662,407 - LysE DVU0593 0.35 -1.4 662,452 - LysE DVU0593 0.42 +1.8 662,468 + LysE DVU0593 0.45 -0.0 662,491 - LysE DVU0593 0.48 +1.3 662,545 - LysE DVU0593 0.57 -0.5 662,641 - LysE DVU0593 0.72 -0.6 662,728 - LysE DVU0593 0.86 +2.0 662,780 - +0.8 662,787 + +0.5 662,795 - +1.0 662,837 + +0.7 662,842 + +0.0 662,916 + -1.9 662,924 - +0.3 662,926 + -0.5 662,926 + -0.2 662,989 - -0.1 663,026 + iciA DVU0594 0.13 -1.0 663,034 - iciA DVU0594 0.14 +0.4 663,036 + iciA DVU0594 0.14 -2.2 663,061 + iciA DVU0594 0.17 +0.2 663,069 - iciA DVU0594 0.18 -0.2 663,074 - iciA DVU0594 0.18 -0.8 663,076 + iciA DVU0594 0.18 -0.7 663,111 + iciA DVU0594 0.22 -0.0 663,117 + iciA DVU0594 0.23 -0.9 663,119 - iciA DVU0594 0.23 +1.4 663,119 - iciA DVU0594 0.23 -0.4 663,139 + iciA DVU0594 0.25 -0.2 663,147 - iciA DVU0594 0.26 -0.0 663,173 - iciA DVU0594 0.29 -1.6 663,196 + iciA DVU0594 0.32 -0.0 663,370 + iciA DVU0594 0.51 +0.0 663,378 - iciA DVU0594 0.52 -0.9 663,383 - iciA DVU0594 0.52 +0.3 663,383 - iciA DVU0594 0.52 -0.1 663,383 - iciA DVU0594 0.52 -0.1 663,407 - iciA DVU0594 0.55 +2.3 663,454 - iciA DVU0594 0.60 -0.4 663,626 - iciA DVU0594 0.79 +0.3 663,714 - iciA DVU0594 0.89 -0.8 663,790 - +1.0 663,790 - +0.3 663,790 - -0.2 663,808 + -0.3
Or see this region's nucleotide sequence