Experiment: MoYLS4 with Nickel Chloride 0.75mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU3349 and oorD are separated by 3 nucleotides oorD and queA are separated by 363 nucleotides queA and DVU3352 are separated by 112 nucleotides DVU3352 and coaBC overlap by 1 nucleotides
DVU3349: DVU3349 - pyruvate flavodoxin/ferredoxin oxidoreductase, thiamine diP-binding domain protein (TIGR), at 3,520,596 to 3,521,669
DVU3349
DVU3350: oorD - iron-sulfur cluster-binding protein (TIGR), at 3,521,673 to 3,521,906
oorD
DVU3351: queA - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (TIGR), at 3,522,270 to 3,523,385
queA
DVU3352: DVU3352 - lipoprotein, putative (TIGR), at 3,523,498 to 3,524,067
DVU3352
DVU3353: coaBC - phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase (TIGR), at 3,524,067 to 3,525,281
coaBC
Position (kb)
3522
3523
3524 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3521.403 kb on - strand, within DVU3349 at 3521.691 kb on - strand at 3521.716 kb on - strand, within oorD at 3521.745 kb on + strand, within oorD at 3521.851 kb on + strand, within oorD at 3521.854 kb on + strand, within oorD at 3521.854 kb on + strand, within oorD at 3521.859 kb on - strand, within oorD at 3522.051 kb on - strand at 3522.051 kb on - strand at 3522.120 kb on - strand at 3522.122 kb on - strand at 3522.122 kb on - strand at 3522.137 kb on + strand at 3522.137 kb on + strand at 3522.196 kb on + strand at 3522.209 kb on + strand at 3522.209 kb on + strand at 3522.217 kb on - strand at 3522.246 kb on + strand at 3522.254 kb on - strand at 3522.266 kb on + strand at 3522.319 kb on - strand at 3522.366 kb on + strand at 3522.367 kb on + strand at 3522.375 kb on - strand at 3522.400 kb on - strand, within queA at 3522.401 kb on + strand, within queA at 3522.439 kb on - strand, within queA at 3522.480 kb on + strand, within queA at 3522.488 kb on - strand, within queA at 3522.488 kb on - strand, within queA at 3522.490 kb on - strand, within queA at 3522.490 kb on - strand, within queA at 3522.492 kb on + strand, within queA at 3522.513 kb on + strand, within queA at 3522.530 kb on + strand, within queA at 3522.558 kb on + strand, within queA at 3522.558 kb on + strand, within queA at 3522.565 kb on + strand, within queA at 3522.565 kb on + strand, within queA at 3522.565 kb on + strand, within queA at 3522.592 kb on - strand, within queA at 3522.592 kb on - strand, within queA at 3522.621 kb on + strand, within queA at 3522.653 kb on + strand, within queA at 3522.671 kb on - strand, within queA at 3522.680 kb on + strand, within queA at 3522.680 kb on + strand, within queA at 3522.688 kb on - strand, within queA at 3522.692 kb on + strand, within queA at 3522.694 kb on - strand, within queA at 3522.786 kb on + strand, within queA at 3522.813 kb on - strand, within queA at 3522.822 kb on + strand, within queA at 3522.832 kb on - strand, within queA at 3522.887 kb on - strand, within queA at 3522.912 kb on - strand, within queA at 3522.917 kb on + strand, within queA at 3522.925 kb on - strand, within queA at 3522.935 kb on - strand, within queA at 3522.945 kb on - strand, within queA at 3522.958 kb on + strand, within queA at 3522.959 kb on + strand, within queA at 3522.971 kb on + strand, within queA at 3523.004 kb on + strand, within queA at 3523.012 kb on - strand, within queA at 3523.022 kb on + strand, within queA at 3523.032 kb on - strand, within queA at 3523.032 kb on - strand, within queA at 3523.052 kb on + strand, within queA at 3523.057 kb on + strand, within queA at 3523.067 kb on + strand, within queA at 3523.126 kb on + strand, within queA at 3523.127 kb on - strand, within queA at 3523.144 kb on - strand, within queA at 3523.179 kb on - strand, within queA at 3523.260 kb on - strand, within queA at 3523.305 kb on + strand at 3523.338 kb on - strand at 3523.339 kb on - strand at 3523.346 kb on - strand at 3523.374 kb on - strand at 3523.428 kb on - strand at 3523.435 kb on + strand at 3523.444 kb on + strand at 3523.444 kb on + strand at 3523.444 kb on + strand at 3523.456 kb on + strand at 3523.488 kb on + strand at 3523.496 kb on - strand at 3523.535 kb on + strand at 3523.574 kb on + strand, within DVU3352 at 3523.635 kb on + strand, within DVU3352 at 3523.675 kb on + strand, within DVU3352 at 3523.827 kb on + strand, within DVU3352 at 3523.827 kb on + strand, within DVU3352 at 3523.901 kb on + strand, within DVU3352 at 3523.901 kb on + strand, within DVU3352 at 3523.959 kb on + strand, within DVU3352 at 3524.064 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Nickel Chloride 0.75mM remove 3,521,403 - DVU3349 0.75 +0.8 3,521,691 - +0.2 3,521,716 - oorD DVU3350 0.18 +1.3 3,521,745 + oorD DVU3350 0.31 -0.3 3,521,851 + oorD DVU3350 0.76 -0.6 3,521,854 + oorD DVU3350 0.77 -1.2 3,521,854 + oorD DVU3350 0.77 +1.2 3,521,859 - oorD DVU3350 0.79 +0.2 3,522,051 - -3.0 3,522,051 - +0.1 3,522,120 - -0.4 3,522,122 - -0.2 3,522,122 - -0.8 3,522,137 + +1.2 3,522,137 + -1.0 3,522,196 + -0.7 3,522,209 + +1.6 3,522,209 + -0.6 3,522,217 - +1.2 3,522,246 + -0.4 3,522,254 - +0.2 3,522,266 + -0.6 3,522,319 - +0.3 3,522,366 + -0.1 3,522,367 + -0.4 3,522,375 - +0.4 3,522,400 - queA DVU3351 0.12 -0.1 3,522,401 + queA DVU3351 0.12 +0.5 3,522,439 - queA DVU3351 0.15 -0.8 3,522,480 + queA DVU3351 0.19 +3.4 3,522,488 - queA DVU3351 0.20 +0.2 3,522,488 - queA DVU3351 0.20 -0.2 3,522,490 - queA DVU3351 0.20 +0.2 3,522,490 - queA DVU3351 0.20 -1.0 3,522,492 + queA DVU3351 0.20 +1.7 3,522,513 + queA DVU3351 0.22 -0.4 3,522,530 + queA DVU3351 0.23 +0.0 3,522,558 + queA DVU3351 0.26 +0.1 3,522,558 + queA DVU3351 0.26 -0.4 3,522,565 + queA DVU3351 0.26 +0.1 3,522,565 + queA DVU3351 0.26 -1.1 3,522,565 + queA DVU3351 0.26 +0.5 3,522,592 - queA DVU3351 0.29 +0.0 3,522,592 - queA DVU3351 0.29 -0.6 3,522,621 + queA DVU3351 0.31 -0.1 3,522,653 + queA DVU3351 0.34 +0.0 3,522,671 - queA DVU3351 0.36 +0.4 3,522,680 + queA DVU3351 0.37 +0.2 3,522,680 + queA DVU3351 0.37 -0.4 3,522,688 - queA DVU3351 0.37 -1.4 3,522,692 + queA DVU3351 0.38 +0.1 3,522,694 - queA DVU3351 0.38 +1.6 3,522,786 + queA DVU3351 0.46 -0.7 3,522,813 - queA DVU3351 0.49 -0.8 3,522,822 + queA DVU3351 0.49 +0.1 3,522,832 - queA DVU3351 0.50 +0.5 3,522,887 - queA DVU3351 0.55 +0.2 3,522,912 - queA DVU3351 0.58 -0.3 3,522,917 + queA DVU3351 0.58 -0.4 3,522,925 - queA DVU3351 0.59 +1.4 3,522,935 - queA DVU3351 0.60 -0.3 3,522,945 - queA DVU3351 0.60 +0.1 3,522,958 + queA DVU3351 0.62 +0.1 3,522,959 + queA DVU3351 0.62 +0.4 3,522,971 + queA DVU3351 0.63 +0.8 3,523,004 + queA DVU3351 0.66 +0.2 3,523,012 - queA DVU3351 0.66 +1.2 3,523,022 + queA DVU3351 0.67 +0.3 3,523,032 - queA DVU3351 0.68 -0.3 3,523,032 - queA DVU3351 0.68 +0.1 3,523,052 + queA DVU3351 0.70 +0.5 3,523,057 + queA DVU3351 0.71 -1.4 3,523,067 + queA DVU3351 0.71 -1.6 3,523,126 + queA DVU3351 0.77 +0.5 3,523,127 - queA DVU3351 0.77 +0.8 3,523,144 - queA DVU3351 0.78 +0.4 3,523,179 - queA DVU3351 0.81 +0.7 3,523,260 - queA DVU3351 0.89 +0.7 3,523,305 + -0.0 3,523,338 - -1.2 3,523,339 - +0.2 3,523,346 - -0.2 3,523,374 - +0.2 3,523,428 - -0.3 3,523,435 + -0.4 3,523,444 + +0.6 3,523,444 + +0.2 3,523,444 + +0.4 3,523,456 + -1.3 3,523,488 + +0.3 3,523,496 - +0.2 3,523,535 + +0.0 3,523,574 + DVU3352 0.13 +0.5 3,523,635 + DVU3352 0.24 -0.2 3,523,675 + DVU3352 0.31 -0.3 3,523,827 + DVU3352 0.58 -0.7 3,523,827 + DVU3352 0.58 +0.4 3,523,901 + DVU3352 0.71 -0.1 3,523,901 + DVU3352 0.71 +0.3 3,523,959 + DVU3352 0.81 -0.6 3,524,064 + +0.9
Or see this region's nucleotide sequence