Experiment: MoYLS4 with Nickel Chloride 0.75mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt selD and DVU1333 are separated by 27 nucleotides DVU1333 and tRNA-Leu overlap by 18 nucleotides tRNA-Leu and tig are separated by 78 nucleotides tig and clpP are separated by 186 nucleotides clpP and clpX are separated by 10 nucleotides
DVU1332: selD - selenide, water dikinase, selenocysteine-containing (TIGR), at 1,408,431 to 1,409,486
selD
DVU1333: DVU1333 - hypothetical protein (TIGR), at 1,409,514 to 1,409,639
DVU1333
DVU_tRNA-Leu-3: tRNA-Leu - tRNA with anticodon TAG for Leu (VIMSS-AUTO), at 1,409,622 to 1,409,706
tRNA-Leu
DVU1334: tig - trigger factor (TIGR), at 1,409,785 to 1,411,086
tig
DVU1335: clpP - ATP-dependent Clp protease, proteolytic subunit (TIGR), at 1,411,273 to 1,411,878
clpP
DVU1336: clpX - ATP-dependent Clp protease, ATP-binding subunit ClpX (TIGR), at 1,411,889 to 1,413,142
clpX
Position (kb)
1409
1410
1411
1412 Strain fitness (log2 ratio)
-1
0
1
2 at 1408.830 kb on + strand, within selD at 1408.838 kb on - strand, within selD at 1408.838 kb on - strand, within selD at 1408.852 kb on - strand, within selD at 1408.895 kb on + strand, within selD at 1408.928 kb on + strand, within selD at 1408.964 kb on + strand, within selD at 1409.011 kb on + strand, within selD at 1409.057 kb on + strand, within selD at 1409.060 kb on - strand, within selD at 1409.070 kb on - strand, within selD at 1409.070 kb on - strand, within selD at 1409.075 kb on + strand, within selD at 1409.125 kb on + strand, within selD at 1409.127 kb on - strand, within selD at 1409.219 kb on - strand, within selD at 1409.395 kb on - strand at 1409.450 kb on + strand at 1409.471 kb on + strand at 1409.480 kb on + strand at 1409.491 kb on + strand at 1409.706 kb on + strand at 1409.713 kb on + strand at 1409.739 kb on - strand at 1411.081 kb on + strand at 1411.081 kb on + strand at 1411.081 kb on + strand at 1411.081 kb on + strand at 1411.081 kb on + strand at 1411.081 kb on + strand at 1411.089 kb on - strand at 1411.089 kb on - strand at 1411.089 kb on - strand at 1411.104 kb on + strand at 1411.544 kb on + strand, within clpP at 1411.570 kb on - strand, within clpP at 1411.570 kb on - strand, within clpP at 1411.722 kb on - strand, within clpP at 1411.761 kb on + strand, within clpP at 1411.796 kb on + strand, within clpP at 1411.804 kb on - strand, within clpP at 1411.832 kb on - strand at 1411.982 kb on + strand at 1411.990 kb on - strand at 1412.033 kb on + strand, within clpX at 1412.038 kb on - strand, within clpX
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Nickel Chloride 0.75mM remove 1,408,830 + selD DVU1332 0.38 -0.4 1,408,838 - selD DVU1332 0.39 -0.4 1,408,838 - selD DVU1332 0.39 +0.9 1,408,852 - selD DVU1332 0.40 -0.1 1,408,895 + selD DVU1332 0.44 -0.2 1,408,928 + selD DVU1332 0.47 -0.8 1,408,964 + selD DVU1332 0.50 -0.5 1,409,011 + selD DVU1332 0.55 -0.5 1,409,057 + selD DVU1332 0.59 -0.1 1,409,060 - selD DVU1332 0.60 -1.1 1,409,070 - selD DVU1332 0.61 -1.4 1,409,070 - selD DVU1332 0.61 -0.5 1,409,075 + selD DVU1332 0.61 -0.4 1,409,125 + selD DVU1332 0.66 +1.3 1,409,127 - selD DVU1332 0.66 +1.1 1,409,219 - selD DVU1332 0.75 -0.2 1,409,395 - -0.4 1,409,450 + -0.4 1,409,471 + +0.4 1,409,480 + -0.2 1,409,491 + +0.5 1,409,706 + +0.0 1,409,713 + -0.6 1,409,739 - -1.0 1,411,081 + -0.3 1,411,081 + +1.0 1,411,081 + -0.9 1,411,081 + -1.0 1,411,081 + -0.5 1,411,081 + +0.6 1,411,089 - +0.2 1,411,089 - -0.3 1,411,089 - +0.5 1,411,104 + +0.1 1,411,544 + clpP DVU1335 0.45 -1.5 1,411,570 - clpP DVU1335 0.49 -0.9 1,411,570 - clpP DVU1335 0.49 +0.8 1,411,722 - clpP DVU1335 0.74 -0.1 1,411,761 + clpP DVU1335 0.81 +2.4 1,411,796 + clpP DVU1335 0.86 +2.4 1,411,804 - clpP DVU1335 0.88 -0.8 1,411,832 - -0.3 1,411,982 + -1.3 1,411,990 - -0.8 1,412,033 + clpX DVU1336 0.11 +0.1 1,412,038 - clpX DVU1336 0.12 -0.5
Or see this region's nucleotide sequence