Experiment: Recovery broth with Hygromycin B 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16680 and BBR_RS16685 are separated by 58 nucleotides BBR_RS16685 and BBR_RS16690 are separated by 132 nucleotides
BBR_RS16680: BBR_RS16680 - chorismate mutase, at 1,513,388 to 1,514,368
_RS16680
BBR_RS16685: BBR_RS16685 - alcohol dehydrogenase, at 1,514,427 to 1,514,867
_RS16685
BBR_RS16690: BBR_RS16690 - translational GTPase TypA, at 1,515,000 to 1,516,931
_RS16690
Position (kb)
1514
1515 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1513.431 kb on - strand at 1513.432 kb on + strand at 1513.438 kb on - strand at 1513.449 kb on + strand at 1513.450 kb on - strand at 1513.485 kb on + strand at 1513.527 kb on + strand, within BBR_RS16680 at 1513.527 kb on - strand, within BBR_RS16680 at 1513.533 kb on + strand, within BBR_RS16680 at 1513.533 kb on + strand, within BBR_RS16680 at 1513.535 kb on - strand, within BBR_RS16680 at 1513.541 kb on - strand, within BBR_RS16680 at 1513.541 kb on - strand, within BBR_RS16680 at 1513.544 kb on + strand, within BBR_RS16680 at 1513.544 kb on + strand, within BBR_RS16680 at 1513.583 kb on - strand, within BBR_RS16680 at 1513.583 kb on - strand, within BBR_RS16680 at 1513.648 kb on - strand, within BBR_RS16680 at 1513.686 kb on - strand, within BBR_RS16680 at 1513.686 kb on - strand, within BBR_RS16680 at 1513.717 kb on + strand, within BBR_RS16680 at 1513.717 kb on + strand, within BBR_RS16680 at 1513.733 kb on - strand, within BBR_RS16680 at 1513.733 kb on - strand, within BBR_RS16680 at 1513.773 kb on + strand, within BBR_RS16680 at 1513.878 kb on + strand, within BBR_RS16680 at 1513.894 kb on + strand, within BBR_RS16680 at 1513.895 kb on - strand, within BBR_RS16680 at 1513.902 kb on - strand, within BBR_RS16680 at 1513.905 kb on - strand, within BBR_RS16680 at 1513.905 kb on - strand, within BBR_RS16680 at 1513.929 kb on - strand, within BBR_RS16680 at 1513.995 kb on + strand, within BBR_RS16680 at 1514.022 kb on - strand, within BBR_RS16680 at 1514.022 kb on - strand, within BBR_RS16680 at 1514.022 kb on - strand, within BBR_RS16680 at 1514.072 kb on + strand, within BBR_RS16680 at 1514.117 kb on + strand, within BBR_RS16680 at 1514.125 kb on - strand, within BBR_RS16680 at 1514.125 kb on - strand, within BBR_RS16680 at 1514.152 kb on - strand, within BBR_RS16680 at 1514.152 kb on - strand, within BBR_RS16680 at 1514.175 kb on - strand, within BBR_RS16680 at 1514.176 kb on - strand, within BBR_RS16680 at 1514.213 kb on - strand, within BBR_RS16680 at 1514.213 kb on - strand, within BBR_RS16680 at 1514.273 kb on - strand at 1514.371 kb on + strand at 1514.439 kb on + strand at 1514.452 kb on - strand at 1514.454 kb on + strand at 1514.480 kb on + strand, within BBR_RS16685 at 1514.485 kb on + strand, within BBR_RS16685 at 1514.825 kb on - strand at 1514.861 kb on - strand at 1514.889 kb on - strand at 1514.895 kb on - strand at 1514.919 kb on + strand at 1514.923 kb on - strand at 1514.927 kb on - strand at 1514.972 kb on - strand at 1515.009 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Hygromycin B 0.2 mM remove 1,513,431 - +1.0 1,513,432 + +0.2 1,513,438 - +0.7 1,513,449 + +0.3 1,513,450 - -0.2 1,513,485 + +0.2 1,513,527 + BBR_RS16680 0.14 +0.5 1,513,527 - BBR_RS16680 0.14 -1.2 1,513,533 + BBR_RS16680 0.15 +1.2 1,513,533 + BBR_RS16680 0.15 -0.2 1,513,535 - BBR_RS16680 0.15 +0.6 1,513,541 - BBR_RS16680 0.16 +0.6 1,513,541 - BBR_RS16680 0.16 -0.8 1,513,544 + BBR_RS16680 0.16 +0.7 1,513,544 + BBR_RS16680 0.16 +0.1 1,513,583 - BBR_RS16680 0.20 +0.3 1,513,583 - BBR_RS16680 0.20 +0.9 1,513,648 - BBR_RS16680 0.27 +0.1 1,513,686 - BBR_RS16680 0.30 -0.3 1,513,686 - BBR_RS16680 0.30 -2.8 1,513,717 + BBR_RS16680 0.34 -0.2 1,513,717 + BBR_RS16680 0.34 -1.2 1,513,733 - BBR_RS16680 0.35 +0.0 1,513,733 - BBR_RS16680 0.35 -0.0 1,513,773 + BBR_RS16680 0.39 +0.1 1,513,878 + BBR_RS16680 0.50 +0.3 1,513,894 + BBR_RS16680 0.52 -0.2 1,513,895 - BBR_RS16680 0.52 -0.0 1,513,902 - BBR_RS16680 0.52 +0.8 1,513,905 - BBR_RS16680 0.53 -0.3 1,513,905 - BBR_RS16680 0.53 -0.4 1,513,929 - BBR_RS16680 0.55 -1.8 1,513,995 + BBR_RS16680 0.62 +0.3 1,514,022 - BBR_RS16680 0.65 -0.3 1,514,022 - BBR_RS16680 0.65 -1.3 1,514,022 - BBR_RS16680 0.65 -0.6 1,514,072 + BBR_RS16680 0.70 -0.3 1,514,117 + BBR_RS16680 0.74 +0.2 1,514,125 - BBR_RS16680 0.75 +0.2 1,514,125 - BBR_RS16680 0.75 +0.4 1,514,152 - BBR_RS16680 0.78 -0.5 1,514,152 - BBR_RS16680 0.78 +0.5 1,514,175 - BBR_RS16680 0.80 +0.3 1,514,176 - BBR_RS16680 0.80 -0.3 1,514,213 - BBR_RS16680 0.84 +0.6 1,514,213 - BBR_RS16680 0.84 -0.6 1,514,273 - +0.3 1,514,371 + +0.4 1,514,439 + +0.1 1,514,452 - +0.3 1,514,454 + +0.3 1,514,480 + BBR_RS16685 0.12 -1.8 1,514,485 + BBR_RS16685 0.13 -1.8 1,514,825 - -0.5 1,514,861 - +0.7 1,514,889 - +0.0 1,514,895 - +0.9 1,514,919 + +1.3 1,514,923 - -1.7 1,514,927 - -0.1 1,514,972 - -0.1 1,515,009 - +0.6
Or see this region's nucleotide sequence