Experiment: Recovery broth with Hygromycin B 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15045 and BBR_RS15050 are separated by 82 nucleotides BBR_RS15050 and BBR_RS15055 overlap by 1 nucleotides BBR_RS15055 and BBR_RS15060 are separated by 95 nucleotides
BBR_RS15045: BBR_RS15045 - histidine phosphatase family protein, at 1,144,866 to 1,145,540
_RS15045
BBR_RS15050: BBR_RS15050 - DUF948 domain-containing protein, at 1,145,623 to 1,146,027
_RS15050
BBR_RS15055: BBR_RS15055 - hypothetical protein, at 1,146,027 to 1,146,263
_RS15055
BBR_RS15060: BBR_RS15060 - alanine--tRNA ligase, at 1,146,359 to 1,149,037
_RS15060
Position (kb)
1146
1147 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1145.115 kb on + strand, within BBR_RS15045 at 1145.155 kb on + strand, within BBR_RS15045 at 1145.155 kb on - strand, within BBR_RS15045 at 1145.171 kb on + strand, within BBR_RS15045 at 1145.171 kb on + strand, within BBR_RS15045 at 1145.171 kb on + strand, within BBR_RS15045 at 1145.171 kb on + strand, within BBR_RS15045 at 1145.192 kb on - strand, within BBR_RS15045 at 1145.200 kb on + strand, within BBR_RS15045 at 1145.207 kb on + strand, within BBR_RS15045 at 1145.336 kb on - strand, within BBR_RS15045 at 1145.365 kb on + strand, within BBR_RS15045 at 1145.407 kb on + strand, within BBR_RS15045 at 1145.434 kb on + strand, within BBR_RS15045 at 1145.434 kb on + strand, within BBR_RS15045 at 1145.434 kb on + strand, within BBR_RS15045 at 1145.434 kb on + strand, within BBR_RS15045 at 1145.434 kb on + strand, within BBR_RS15045 at 1145.442 kb on - strand, within BBR_RS15045 at 1145.457 kb on + strand, within BBR_RS15045 at 1145.464 kb on - strand, within BBR_RS15045 at 1145.481 kb on - strand at 1145.483 kb on - strand at 1145.483 kb on - strand at 1145.492 kb on + strand at 1145.548 kb on - strand at 1145.567 kb on + strand at 1145.585 kb on + strand at 1145.588 kb on - strand at 1145.588 kb on - strand at 1145.588 kb on - strand at 1145.599 kb on + strand at 1145.750 kb on + strand, within BBR_RS15050 at 1145.771 kb on + strand, within BBR_RS15050 at 1145.938 kb on + strand, within BBR_RS15050 at 1145.953 kb on + strand, within BBR_RS15050 at 1146.038 kb on + strand at 1146.052 kb on + strand, within BBR_RS15055 at 1146.085 kb on + strand, within BBR_RS15055 at 1146.090 kb on + strand, within BBR_RS15055 at 1146.183 kb on + strand, within BBR_RS15055 at 1146.218 kb on + strand, within BBR_RS15055 at 1146.239 kb on + strand, within BBR_RS15055 at 1146.274 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Hygromycin B 0.2 mM remove 1,145,115 + BBR_RS15045 0.37 +0.5 1,145,155 + BBR_RS15045 0.43 +0.2 1,145,155 - BBR_RS15045 0.43 +0.4 1,145,171 + BBR_RS15045 0.45 +0.6 1,145,171 + BBR_RS15045 0.45 +0.7 1,145,171 + BBR_RS15045 0.45 +1.8 1,145,171 + BBR_RS15045 0.45 +0.1 1,145,192 - BBR_RS15045 0.48 +0.2 1,145,200 + BBR_RS15045 0.49 -0.5 1,145,207 + BBR_RS15045 0.51 +0.2 1,145,336 - BBR_RS15045 0.70 -0.2 1,145,365 + BBR_RS15045 0.74 -0.1 1,145,407 + BBR_RS15045 0.80 +0.0 1,145,434 + BBR_RS15045 0.84 -0.4 1,145,434 + BBR_RS15045 0.84 +0.1 1,145,434 + BBR_RS15045 0.84 -1.1 1,145,434 + BBR_RS15045 0.84 -0.9 1,145,434 + BBR_RS15045 0.84 -1.4 1,145,442 - BBR_RS15045 0.85 +0.2 1,145,457 + BBR_RS15045 0.88 +0.3 1,145,464 - BBR_RS15045 0.89 -1.1 1,145,481 - +0.9 1,145,483 - +1.3 1,145,483 - -0.6 1,145,492 + -0.7 1,145,548 - +0.3 1,145,567 + -0.2 1,145,585 + +0.1 1,145,588 - -1.1 1,145,588 - +0.6 1,145,588 - +1.5 1,145,599 + -0.7 1,145,750 + BBR_RS15050 0.31 -1.9 1,145,771 + BBR_RS15050 0.37 -0.5 1,145,938 + BBR_RS15050 0.78 -1.9 1,145,953 + BBR_RS15050 0.81 -0.0 1,146,038 + -1.5 1,146,052 + BBR_RS15055 0.11 -1.2 1,146,085 + BBR_RS15055 0.24 +0.9 1,146,090 + BBR_RS15055 0.27 +0.2 1,146,183 + BBR_RS15055 0.66 -2.3 1,146,218 + BBR_RS15055 0.81 -1.7 1,146,239 + BBR_RS15055 0.89 -2.6 1,146,274 + -0.2
Or see this region's nucleotide sequence