Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS14280

Experiment: Recovery broth with Hygromycin B 0.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBBR_RS14275 and BBR_RS14280 are separated by 147 nucleotidesBBR_RS14280 and BBR_RS14285 are separated by 165 nucleotides BBR_RS14275: BBR_RS14275 - 30S ribosomal protein S1, at 977,009 to 978,484 _RS14275 BBR_RS14280: BBR_RS14280 - dephospho-CoA kinase, at 978,632 to 979,258 _RS14280 BBR_RS14285: BBR_RS14285 - excinuclease ABC subunit UvrB, at 979,424 to 981,535 _RS14285 Position (kb) 978 979 980Strain fitness (log2 ratio) -3 -2 -1 0 1at 977.969 kb on + strand, within BBR_RS14275at 978.593 kb on - strandat 978.626 kb on - strandat 979.109 kb on + strand, within BBR_RS14280at 979.169 kb on + strand, within BBR_RS14280at 979.234 kb on + strandat 979.453 kb on + strandat 979.495 kb on + strandat 979.509 kb on - strandat 979.550 kb on + strandat 979.558 kb on - strandat 979.564 kb on + strandat 979.749 kb on - strand, within BBR_RS14285at 979.772 kb on - strand, within BBR_RS14285at 979.780 kb on - strand, within BBR_RS14285at 979.859 kb on - strand, within BBR_RS14285at 980.031 kb on - strand, within BBR_RS14285at 980.040 kb on - strand, within BBR_RS14285at 980.042 kb on + strand, within BBR_RS14285at 980.175 kb on - strand, within BBR_RS14285at 980.181 kb on + strand, within BBR_RS14285at 980.253 kb on + strand, within BBR_RS14285

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Per-strain Table

Position Strand Gene LocusTag Fraction Recovery broth with Hygromycin B 0.2 mM
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977,969 + BBR_RS14275 0.65 -3.0
978,593 - +0.2
978,626 - -0.2
979,109 + BBR_RS14280 0.76 +0.5
979,169 + BBR_RS14280 0.86 +1.2
979,234 + -0.5
979,453 + +0.2
979,495 + +1.6
979,509 - -1.0
979,550 + +0.2
979,558 - +0.8
979,564 + -0.5
979,749 - BBR_RS14285 0.15 +1.0
979,772 - BBR_RS14285 0.16 -0.4
979,780 - BBR_RS14285 0.17 -0.1
979,859 - BBR_RS14285 0.21 +0.0
980,031 - BBR_RS14285 0.29 -0.6
980,040 - BBR_RS14285 0.29 -0.5
980,042 + BBR_RS14285 0.29 -0.5
980,175 - BBR_RS14285 0.36 -1.6
980,181 + BBR_RS14285 0.36 -0.9
980,253 + BBR_RS14285 0.39 -2.8

Or see this region's nucleotide sequence