Experiment: Recovery broth with Hygromycin B 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14265 and BBR_RS14270 are separated by 104 nucleotides BBR_RS14270 and BBR_RS14275 are separated by 249 nucleotides
BBR_RS14265: BBR_RS14265 - Solute-binding protein of ABC transporter system for metals, at 974,604 to 975,776
_RS14265
BBR_RS14270: BBR_RS14270 - bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, at 975,881 to 976,759
_RS14270
BBR_RS14275: BBR_RS14275 - 30S ribosomal protein S1, at 977,009 to 978,484
_RS14275
Position (kb) 975
976
977 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 974.893 kb on - strand, within BBR_RS14265 at 974.903 kb on + strand, within BBR_RS14265 at 974.915 kb on - strand, within BBR_RS14265 at 974.926 kb on + strand, within BBR_RS14265 at 975.051 kb on + strand, within BBR_RS14265 at 975.102 kb on - strand, within BBR_RS14265 at 975.102 kb on - strand, within BBR_RS14265 at 975.113 kb on - strand, within BBR_RS14265 at 975.151 kb on + strand, within BBR_RS14265 at 975.159 kb on + strand, within BBR_RS14265 at 975.191 kb on - strand, within BBR_RS14265 at 975.288 kb on + strand, within BBR_RS14265 at 975.295 kb on + strand, within BBR_RS14265 at 975.296 kb on - strand, within BBR_RS14265 at 975.324 kb on + strand, within BBR_RS14265 at 975.469 kb on + strand, within BBR_RS14265 at 975.486 kb on + strand, within BBR_RS14265 at 975.489 kb on - strand, within BBR_RS14265 at 975.489 kb on - strand, within BBR_RS14265 at 975.516 kb on - strand, within BBR_RS14265 at 975.611 kb on + strand, within BBR_RS14265 at 975.674 kb on - strand at 975.716 kb on + strand at 975.818 kb on + strand at 975.860 kb on + strand at 975.860 kb on + strand at 975.875 kb on + strand at 975.875 kb on + strand at 975.875 kb on + strand at 976.749 kb on + strand at 976.757 kb on - strand at 976.766 kb on + strand at 976.791 kb on + strand at 976.813 kb on + strand at 976.848 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Hygromycin B 0.2 mM remove 974,893 - BBR_RS14265 0.25 +0.5 974,903 + BBR_RS14265 0.25 -2.6 974,915 - BBR_RS14265 0.27 -1.3 974,926 + BBR_RS14265 0.27 -0.6 975,051 + BBR_RS14265 0.38 -2.9 975,102 - BBR_RS14265 0.42 -0.6 975,102 - BBR_RS14265 0.42 -0.3 975,113 - BBR_RS14265 0.43 -2.4 975,151 + BBR_RS14265 0.47 +0.3 975,159 + BBR_RS14265 0.47 -1.7 975,191 - BBR_RS14265 0.50 -1.0 975,288 + BBR_RS14265 0.58 -2.1 975,295 + BBR_RS14265 0.59 -2.3 975,296 - BBR_RS14265 0.59 -0.9 975,324 + BBR_RS14265 0.61 -1.2 975,469 + BBR_RS14265 0.74 +0.1 975,486 + BBR_RS14265 0.75 +0.3 975,489 - BBR_RS14265 0.75 -3.0 975,489 - BBR_RS14265 0.75 +0.2 975,516 - BBR_RS14265 0.78 -0.5 975,611 + BBR_RS14265 0.86 +0.1 975,674 - -1.0 975,716 + -0.5 975,818 + -2.1 975,860 + -0.1 975,860 + -0.4 975,875 + -0.8 975,875 + +0.9 975,875 + +0.2 976,749 + +0.7 976,757 - -0.3 976,766 + +0.7 976,791 + +0.7 976,813 + -0.1 976,848 + +0.1
Or see this region's nucleotide sequence