Experiment: Recovery broth with Hygromycin B 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14250 and BBR_RS14255 are separated by 72 nucleotides BBR_RS14255 and BBR_RS14260 are separated by 90 nucleotides BBR_RS14260 and BBR_RS14265 are separated by 157 nucleotides
BBR_RS14250: BBR_RS14250 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 972,068 to 972,577
_RS14250
BBR_RS14255: BBR_RS14255 - metal ABC transporter permease, at 972,650 to 973,483
_RS14255
BBR_RS14260: BBR_RS14260 - ABC transporter ATP-binding protein, at 973,574 to 974,446
_RS14260
BBR_RS14265: BBR_RS14265 - Solute-binding protein of ABC transporter system for metals, at 974,604 to 975,776
_RS14265
Position (kb) 973
974
975 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 972.662 kb on + strand at 972.672 kb on + strand at 972.692 kb on - strand at 972.692 kb on - strand at 973.555 kb on - strand at 973.560 kb on - strand at 973.595 kb on + strand at 973.727 kb on - strand, within BBR_RS14260 at 973.761 kb on - strand, within BBR_RS14260 at 974.456 kb on - strand at 974.523 kb on - strand at 974.562 kb on + strand at 974.584 kb on - strand at 974.608 kb on - strand at 974.628 kb on - strand at 974.646 kb on - strand at 974.648 kb on + strand at 974.659 kb on + strand at 974.662 kb on - strand at 974.662 kb on - strand at 974.682 kb on - strand at 974.738 kb on - strand, within BBR_RS14265 at 974.776 kb on - strand, within BBR_RS14265 at 974.834 kb on + strand, within BBR_RS14265 at 974.841 kb on + strand, within BBR_RS14265 at 974.860 kb on - strand, within BBR_RS14265 at 974.893 kb on - strand, within BBR_RS14265 at 974.903 kb on + strand, within BBR_RS14265 at 974.915 kb on - strand, within BBR_RS14265 at 974.926 kb on + strand, within BBR_RS14265 at 975.051 kb on + strand, within BBR_RS14265 at 975.102 kb on - strand, within BBR_RS14265 at 975.102 kb on - strand, within BBR_RS14265 at 975.113 kb on - strand, within BBR_RS14265 at 975.151 kb on + strand, within BBR_RS14265 at 975.159 kb on + strand, within BBR_RS14265 at 975.191 kb on - strand, within BBR_RS14265 at 975.288 kb on + strand, within BBR_RS14265 at 975.295 kb on + strand, within BBR_RS14265 at 975.296 kb on - strand, within BBR_RS14265 at 975.324 kb on + strand, within BBR_RS14265
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Hygromycin B 0.2 mM remove 972,662 + -0.8 972,672 + +0.0 972,692 - +1.2 972,692 - -1.0 973,555 - -0.0 973,560 - +0.4 973,595 + -0.2 973,727 - BBR_RS14260 0.18 -1.6 973,761 - BBR_RS14260 0.21 -1.7 974,456 - +1.8 974,523 - -0.4 974,562 + +0.5 974,584 - -0.4 974,608 - -1.0 974,628 - -0.3 974,646 - -1.3 974,648 + +0.8 974,659 + -1.0 974,662 - -0.3 974,662 - +0.8 974,682 - -0.1 974,738 - BBR_RS14265 0.11 -0.4 974,776 - BBR_RS14265 0.15 -1.9 974,834 + BBR_RS14265 0.20 -0.2 974,841 + BBR_RS14265 0.20 -2.2 974,860 - BBR_RS14265 0.22 +0.1 974,893 - BBR_RS14265 0.25 +0.5 974,903 + BBR_RS14265 0.25 -2.6 974,915 - BBR_RS14265 0.27 -1.3 974,926 + BBR_RS14265 0.27 -0.6 975,051 + BBR_RS14265 0.38 -2.9 975,102 - BBR_RS14265 0.42 -0.6 975,102 - BBR_RS14265 0.42 -0.3 975,113 - BBR_RS14265 0.43 -2.4 975,151 + BBR_RS14265 0.47 +0.3 975,159 + BBR_RS14265 0.47 -1.7 975,191 - BBR_RS14265 0.50 -1.0 975,288 + BBR_RS14265 0.58 -2.1 975,295 + BBR_RS14265 0.59 -2.3 975,296 - BBR_RS14265 0.59 -0.9 975,324 + BBR_RS14265 0.61 -1.2
Or see this region's nucleotide sequence