Experiment: RCH2_defined_glucose_5mM with Adenine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_003679 and ABIE53_003680 are separated by 180 nucleotides ABIE53_003680 and ABIE53_003862 are separated by 224 nucleotides ABIE53_003862 and ABIE53_003681 are separated by 19 nucleotides ABIE53_003681 and ABIE53_003682 are separated by 70 nucleotides
ABIE53_003679: ABIE53_003679 - putative flavoprotein (TIGR03862 family), at 4,135,341 to 4,136,633
_003679
ABIE53_003680: ABIE53_003680 - methylase of polypeptide subunit release factors, at 4,136,814 to 4,137,941
_003680
ABIE53_003862: ABIE53_003862 - tRNA-Thr, at 4,138,166 to 4,138,241
_003862
ABIE53_003681: ABIE53_003681 - stringent starvation protein B, at 4,138,261 to 4,138,800
_003681
ABIE53_003682: ABIE53_003682 - RNA polymerase-associated protein, at 4,138,871 to 4,139,482
_003682
Position (kb) 4136
4137
4138 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 4136.004 kb on + strand, within ABIE53_003679 at 4136.004 kb on + strand, within ABIE53_003679 at 4136.004 kb on + strand, within ABIE53_003679 at 4136.004 kb on + strand, within ABIE53_003679 at 4136.004 kb on + strand, within ABIE53_003679 at 4136.004 kb on + strand, within ABIE53_003679 at 4136.005 kb on - strand, within ABIE53_003679 at 4136.005 kb on - strand, within ABIE53_003679 at 4136.005 kb on - strand, within ABIE53_003679 at 4136.005 kb on - strand, within ABIE53_003679 at 4136.005 kb on - strand, within ABIE53_003679 at 4136.136 kb on + strand, within ABIE53_003679 at 4136.136 kb on + strand, within ABIE53_003679 at 4136.136 kb on + strand, within ABIE53_003679 at 4136.136 kb on + strand, within ABIE53_003679 at 4136.136 kb on + strand, within ABIE53_003679 at 4136.136 kb on + strand, within ABIE53_003679 at 4136.137 kb on - strand, within ABIE53_003679 at 4136.137 kb on - strand, within ABIE53_003679 at 4136.137 kb on - strand, within ABIE53_003679 at 4136.137 kb on - strand, within ABIE53_003679 at 4136.137 kb on - strand, within ABIE53_003679 at 4136.308 kb on + strand, within ABIE53_003679 at 4136.309 kb on - strand, within ABIE53_003679 at 4136.309 kb on - strand, within ABIE53_003679 at 4136.309 kb on - strand, within ABIE53_003679 at 4136.547 kb on + strand at 4136.548 kb on - strand at 4136.548 kb on - strand at 4136.548 kb on - strand at 4136.554 kb on + strand at 4136.555 kb on - strand at 4136.710 kb on + strand at 4136.711 kb on - strand at 4136.753 kb on + strand at 4136.754 kb on - strand at 4136.790 kb on + strand at 4136.790 kb on + strand at 4136.791 kb on - strand at 4136.791 kb on - strand at 4136.791 kb on - strand at 4136.867 kb on + strand at 4136.867 kb on + strand at 4136.867 kb on + strand at 4136.868 kb on - strand at 4136.868 kb on - strand at 4136.915 kb on + strand at 4136.915 kb on + strand at 4136.916 kb on - strand at 4136.916 kb on - strand at 4136.916 kb on - strand at 4137.094 kb on + strand, within ABIE53_003680 at 4137.094 kb on + strand, within ABIE53_003680 at 4137.094 kb on + strand, within ABIE53_003680 at 4137.094 kb on + strand, within ABIE53_003680 at 4137.094 kb on + strand, within ABIE53_003680 at 4137.094 kb on + strand, within ABIE53_003680 at 4137.094 kb on + strand, within ABIE53_003680 at 4137.095 kb on - strand, within ABIE53_003680 at 4137.095 kb on - strand, within ABIE53_003680 at 4137.095 kb on - strand, within ABIE53_003680 at 4137.103 kb on + strand, within ABIE53_003680 at 4137.103 kb on + strand, within ABIE53_003680 at 4137.104 kb on - strand, within ABIE53_003680 at 4137.104 kb on - strand, within ABIE53_003680 at 4137.104 kb on - strand, within ABIE53_003680 at 4137.214 kb on + strand, within ABIE53_003680 at 4137.214 kb on + strand, within ABIE53_003680 at 4137.391 kb on + strand, within ABIE53_003680 at 4137.391 kb on + strand, within ABIE53_003680 at 4137.391 kb on + strand, within ABIE53_003680 at 4137.391 kb on + strand, within ABIE53_003680 at 4137.391 kb on + strand, within ABIE53_003680 at 4137.391 kb on + strand, within ABIE53_003680 at 4137.391 kb on + strand, within ABIE53_003680 at 4137.391 kb on + strand, within ABIE53_003680 at 4137.391 kb on + strand, within ABIE53_003680 at 4137.391 kb on + strand, within ABIE53_003680 at 4137.391 kb on + strand, within ABIE53_003680 at 4137.391 kb on + strand, within ABIE53_003680 at 4137.391 kb on + strand, within ABIE53_003680 at 4137.391 kb on + strand, within ABIE53_003680 at 4137.391 kb on + strand, within ABIE53_003680 at 4137.391 kb on + strand, within ABIE53_003680 at 4137.392 kb on - strand, within ABIE53_003680 at 4137.392 kb on - strand, within ABIE53_003680 at 4137.392 kb on - strand, within ABIE53_003680 at 4137.392 kb on - strand, within ABIE53_003680 at 4137.392 kb on - strand, within ABIE53_003680 at 4137.392 kb on - strand, within ABIE53_003680 at 4137.392 kb on - strand, within ABIE53_003680 at 4137.392 kb on - strand, within ABIE53_003680 at 4137.392 kb on - strand, within ABIE53_003680 at 4137.392 kb on - strand, within ABIE53_003680 at 4137.392 kb on - strand, within ABIE53_003680 at 4137.392 kb on - strand, within ABIE53_003680 at 4137.392 kb on - strand, within ABIE53_003680 at 4137.421 kb on + strand, within ABIE53_003680 at 4137.421 kb on + strand, within ABIE53_003680 at 4137.421 kb on + strand, within ABIE53_003680 at 4137.421 kb on + strand, within ABIE53_003680 at 4137.421 kb on + strand, within ABIE53_003680 at 4137.548 kb on - strand, within ABIE53_003680 at 4137.548 kb on - strand, within ABIE53_003680 at 4137.548 kb on - strand, within ABIE53_003680 at 4137.674 kb on + strand, within ABIE53_003680 at 4137.674 kb on + strand, within ABIE53_003680 at 4137.674 kb on + strand, within ABIE53_003680 at 4137.675 kb on - strand, within ABIE53_003680 at 4137.675 kb on - strand, within ABIE53_003680 at 4137.675 kb on - strand, within ABIE53_003680 at 4137.675 kb on - strand, within ABIE53_003680 at 4138.047 kb on - strand at 4138.057 kb on + strand at 4138.057 kb on + strand at 4138.057 kb on + strand at 4138.057 kb on + strand at 4138.058 kb on - strand at 4138.121 kb on - strand at 4138.257 kb on + strand at 4138.258 kb on - strand at 4138.267 kb on + strand at 4138.267 kb on + strand at 4138.532 kb on + strand, within ABIE53_003681 at 4138.532 kb on + strand, within ABIE53_003681 at 4138.532 kb on + strand, within ABIE53_003681 at 4138.532 kb on + strand, within ABIE53_003681 at 4138.532 kb on + strand, within ABIE53_003681 at 4138.533 kb on - strand, within ABIE53_003681 at 4138.533 kb on - strand, within ABIE53_003681 at 4138.533 kb on - strand, within ABIE53_003681 at 4138.533 kb on - strand, within ABIE53_003681 at 4138.533 kb on - strand, within ABIE53_003681 at 4138.533 kb on - strand, within ABIE53_003681 at 4138.533 kb on - strand, within ABIE53_003681 at 4138.533 kb on - strand, within ABIE53_003681 at 4138.533 kb on - strand, within ABIE53_003681 at 4138.533 kb on - strand, within ABIE53_003681 at 4138.545 kb on + strand, within ABIE53_003681 at 4138.545 kb on + strand, within ABIE53_003681 at 4138.545 kb on + strand, within ABIE53_003681 at 4138.546 kb on - strand, within ABIE53_003681 at 4138.546 kb on - strand, within ABIE53_003681 at 4138.555 kb on - strand, within ABIE53_003681 at 4138.733 kb on + strand, within ABIE53_003681 at 4138.734 kb on - strand, within ABIE53_003681 at 4138.757 kb on + strand at 4138.757 kb on + strand at 4138.757 kb on + strand at 4138.758 kb on - strand at 4138.758 kb on - strand at 4138.758 kb on - strand at 4138.775 kb on + strand at 4138.775 kb on + strand at 4138.775 kb on + strand at 4138.775 kb on + strand at 4138.776 kb on - strand at 4138.776 kb on - strand at 4138.776 kb on - strand at 4138.776 kb on - strand at 4138.776 kb on - strand at 4138.776 kb on - strand at 4138.776 kb on - strand at 4138.776 kb on - strand at 4138.776 kb on - strand at 4138.776 kb on - strand at 4138.776 kb on - strand at 4138.776 kb on - strand at 4138.813 kb on + strand at 4138.814 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose_5mM with Adenine 5 mM remove 4,136,004 + ABIE53_003679 0.51 +1.1 4,136,004 + ABIE53_003679 0.51 -2.3 4,136,004 + ABIE53_003679 0.51 -2.4 4,136,004 + ABIE53_003679 0.51 +1.1 4,136,004 + ABIE53_003679 0.51 -0.6 4,136,004 + ABIE53_003679 0.51 +1.1 4,136,005 - ABIE53_003679 0.51 +0.2 4,136,005 - ABIE53_003679 0.51 +1.1 4,136,005 - ABIE53_003679 0.51 +2.6 4,136,005 - ABIE53_003679 0.51 +0.4 4,136,005 - ABIE53_003679 0.51 -1.0 4,136,136 + ABIE53_003679 0.61 +0.8 4,136,136 + ABIE53_003679 0.61 +0.9 4,136,136 + ABIE53_003679 0.61 +2.0 4,136,136 + ABIE53_003679 0.61 -0.1 4,136,136 + ABIE53_003679 0.61 -0.7 4,136,136 + ABIE53_003679 0.61 -0.5 4,136,137 - ABIE53_003679 0.62 +1.7 4,136,137 - ABIE53_003679 0.62 -0.6 4,136,137 - ABIE53_003679 0.62 +0.2 4,136,137 - ABIE53_003679 0.62 -3.7 4,136,137 - ABIE53_003679 0.62 +1.2 4,136,308 + ABIE53_003679 0.75 -0.6 4,136,309 - ABIE53_003679 0.75 +1.0 4,136,309 - ABIE53_003679 0.75 -0.7 4,136,309 - ABIE53_003679 0.75 -0.4 4,136,547 + -1.6 4,136,548 - +1.1 4,136,548 - -0.2 4,136,548 - +0.1 4,136,554 + +0.0 4,136,555 - +0.2 4,136,710 + +1.6 4,136,711 - +1.4 4,136,753 + +0.1 4,136,754 - -0.5 4,136,790 + -2.1 4,136,790 + +1.8 4,136,791 - -0.6 4,136,791 - +2.0 4,136,791 - -0.3 4,136,867 + +1.7 4,136,867 + -1.7 4,136,867 + +0.7 4,136,868 - -0.9 4,136,868 - +0.7 4,136,915 + +1.1 4,136,915 + -0.2 4,136,916 - +1.9 4,136,916 - -0.7 4,136,916 - -0.2 4,137,094 + ABIE53_003680 0.25 -0.0 4,137,094 + ABIE53_003680 0.25 +0.6 4,137,094 + ABIE53_003680 0.25 +0.7 4,137,094 + ABIE53_003680 0.25 +0.2 4,137,094 + ABIE53_003680 0.25 -1.1 4,137,094 + ABIE53_003680 0.25 +1.1 4,137,094 + ABIE53_003680 0.25 +0.1 4,137,095 - ABIE53_003680 0.25 -1.1 4,137,095 - ABIE53_003680 0.25 -0.8 4,137,095 - ABIE53_003680 0.25 -1.0 4,137,103 + ABIE53_003680 0.26 -0.2 4,137,103 + ABIE53_003680 0.26 +0.1 4,137,104 - ABIE53_003680 0.26 -1.6 4,137,104 - ABIE53_003680 0.26 +1.5 4,137,104 - ABIE53_003680 0.26 -2.5 4,137,214 + ABIE53_003680 0.35 +0.6 4,137,214 + ABIE53_003680 0.35 +0.4 4,137,391 + ABIE53_003680 0.51 +2.6 4,137,391 + ABIE53_003680 0.51 -0.2 4,137,391 + ABIE53_003680 0.51 -0.4 4,137,391 + ABIE53_003680 0.51 +0.4 4,137,391 + ABIE53_003680 0.51 +0.2 4,137,391 + ABIE53_003680 0.51 +0.2 4,137,391 + ABIE53_003680 0.51 -0.2 4,137,391 + ABIE53_003680 0.51 +2.1 4,137,391 + ABIE53_003680 0.51 +0.5 4,137,391 + ABIE53_003680 0.51 +0.7 4,137,391 + ABIE53_003680 0.51 -0.3 4,137,391 + ABIE53_003680 0.51 -1.3 4,137,391 + ABIE53_003680 0.51 -0.5 4,137,391 + ABIE53_003680 0.51 +0.5 4,137,391 + ABIE53_003680 0.51 -1.1 4,137,391 + ABIE53_003680 0.51 +0.6 4,137,392 - ABIE53_003680 0.51 +0.1 4,137,392 - ABIE53_003680 0.51 -1.6 4,137,392 - ABIE53_003680 0.51 -0.3 4,137,392 - ABIE53_003680 0.51 -0.7 4,137,392 - ABIE53_003680 0.51 +1.6 4,137,392 - ABIE53_003680 0.51 -1.3 4,137,392 - ABIE53_003680 0.51 -0.1 4,137,392 - ABIE53_003680 0.51 +0.6 4,137,392 - ABIE53_003680 0.51 -1.0 4,137,392 - ABIE53_003680 0.51 +3.3 4,137,392 - ABIE53_003680 0.51 -0.0 4,137,392 - ABIE53_003680 0.51 -0.4 4,137,392 - ABIE53_003680 0.51 -0.4 4,137,421 + ABIE53_003680 0.54 +0.1 4,137,421 + ABIE53_003680 0.54 -0.0 4,137,421 + ABIE53_003680 0.54 +1.7 4,137,421 + ABIE53_003680 0.54 -0.0 4,137,421 + ABIE53_003680 0.54 -1.4 4,137,548 - ABIE53_003680 0.65 +0.3 4,137,548 - ABIE53_003680 0.65 -0.9 4,137,548 - ABIE53_003680 0.65 +0.7 4,137,674 + ABIE53_003680 0.76 +0.7 4,137,674 + ABIE53_003680 0.76 -0.4 4,137,674 + ABIE53_003680 0.76 -0.0 4,137,675 - ABIE53_003680 0.76 +0.3 4,137,675 - ABIE53_003680 0.76 +1.7 4,137,675 - ABIE53_003680 0.76 +0.1 4,137,675 - ABIE53_003680 0.76 -1.5 4,138,047 - -1.2 4,138,057 + -1.3 4,138,057 + -0.6 4,138,057 + +1.1 4,138,057 + +0.1 4,138,058 - -1.4 4,138,121 - -0.2 4,138,257 + +3.3 4,138,258 - +1.8 4,138,267 + +5.5 4,138,267 + +5.1 4,138,532 + ABIE53_003681 0.50 +3.1 4,138,532 + ABIE53_003681 0.50 +3.5 4,138,532 + ABIE53_003681 0.50 +1.9 4,138,532 + ABIE53_003681 0.50 -0.9 4,138,532 + ABIE53_003681 0.50 +0.6 4,138,533 - ABIE53_003681 0.50 +0.6 4,138,533 - ABIE53_003681 0.50 -0.1 4,138,533 - ABIE53_003681 0.50 +0.4 4,138,533 - ABIE53_003681 0.50 +0.3 4,138,533 - ABIE53_003681 0.50 +0.4 4,138,533 - ABIE53_003681 0.50 -0.2 4,138,533 - ABIE53_003681 0.50 +0.5 4,138,533 - ABIE53_003681 0.50 -0.0 4,138,533 - ABIE53_003681 0.50 +2.1 4,138,533 - ABIE53_003681 0.50 +2.1 4,138,545 + ABIE53_003681 0.53 +1.5 4,138,545 + ABIE53_003681 0.53 -0.3 4,138,545 + ABIE53_003681 0.53 -0.1 4,138,546 - ABIE53_003681 0.53 -1.2 4,138,546 - ABIE53_003681 0.53 +0.9 4,138,555 - ABIE53_003681 0.54 +2.1 4,138,733 + ABIE53_003681 0.87 -0.4 4,138,734 - ABIE53_003681 0.88 -0.3 4,138,757 + +0.1 4,138,757 + +0.2 4,138,757 + -0.3 4,138,758 - +1.1 4,138,758 - +1.3 4,138,758 - -2.9 4,138,775 + -1.4 4,138,775 + -1.1 4,138,775 + -0.4 4,138,775 + -0.4 4,138,776 - +2.4 4,138,776 - +1.1 4,138,776 - +1.5 4,138,776 - +0.4 4,138,776 - +0.4 4,138,776 - -0.1 4,138,776 - -0.4 4,138,776 - -1.4 4,138,776 - +0.2 4,138,776 - +0.8 4,138,776 - +0.5 4,138,776 - +0.3 4,138,813 + +0.0 4,138,814 - +0.1
Or see this region's nucleotide sequence