Experiment: Recovery broth with Ciprofloxacin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13210 and BBR_RS13215 are separated by 3 nucleotides BBR_RS13215 and BBR_RS13220 are separated by 292 nucleotides BBR_RS13220 and BBR_RS13225 are separated by 147 nucleotides
BBR_RS13210: BBR_RS13210 - anion transporter, at 739,864 to 741,042
_RS13210
BBR_RS13215: BBR_RS13215 - hypothetical protein, at 741,046 to 741,267
_RS13215
BBR_RS13220: BBR_RS13220 - hypothetical protein, at 741,560 to 742,018
_RS13220
BBR_RS13225: BBR_RS13225 - MurR/RpiR family transcriptional regulator, at 742,166 to 743,035
_RS13225
Position (kb) 741
742
743 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 740.595 kb on + strand, within BBR_RS13210 at 740.659 kb on + strand, within BBR_RS13210 at 740.672 kb on - strand, within BBR_RS13210 at 740.677 kb on + strand, within BBR_RS13210 at 740.753 kb on - strand, within BBR_RS13210 at 740.770 kb on - strand, within BBR_RS13210 at 740.815 kb on - strand, within BBR_RS13210 at 740.878 kb on - strand, within BBR_RS13210 at 741.000 kb on + strand at 741.072 kb on - strand, within BBR_RS13215 at 741.111 kb on - strand, within BBR_RS13215 at 741.226 kb on + strand, within BBR_RS13215 at 741.276 kb on + strand at 741.278 kb on + strand at 741.278 kb on + strand at 741.284 kb on - strand at 741.286 kb on - strand at 741.478 kb on + strand at 741.478 kb on + strand at 741.486 kb on - strand at 741.486 kb on - strand at 741.493 kb on - strand at 741.500 kb on + strand at 741.500 kb on + strand at 741.618 kb on + strand, within BBR_RS13220 at 741.742 kb on + strand, within BBR_RS13220 at 741.750 kb on - strand, within BBR_RS13220 at 741.751 kb on - strand, within BBR_RS13220 at 741.823 kb on + strand, within BBR_RS13220 at 741.848 kb on - strand, within BBR_RS13220 at 741.850 kb on - strand, within BBR_RS13220 at 741.956 kb on + strand, within BBR_RS13220 at 741.963 kb on + strand, within BBR_RS13220 at 741.963 kb on + strand, within BBR_RS13220 at 741.963 kb on + strand, within BBR_RS13220 at 741.965 kb on + strand, within BBR_RS13220 at 741.988 kb on - strand at 741.990 kb on - strand at 741.990 kb on - strand at 742.006 kb on - strand at 742.023 kb on - strand at 742.041 kb on - strand at 742.072 kb on + strand at 742.096 kb on - strand at 742.115 kb on + strand at 742.115 kb on + strand at 742.133 kb on - strand at 742.137 kb on + strand at 742.151 kb on - strand at 742.158 kb on + strand at 742.174 kb on - strand at 742.190 kb on - strand at 742.205 kb on + strand at 742.237 kb on - strand at 742.246 kb on + strand at 742.248 kb on + strand at 742.254 kb on - strand, within BBR_RS13225 at 742.321 kb on + strand, within BBR_RS13225 at 742.321 kb on + strand, within BBR_RS13225 at 742.342 kb on - strand, within BBR_RS13225 at 742.375 kb on + strand, within BBR_RS13225 at 742.460 kb on + strand, within BBR_RS13225 at 742.466 kb on - strand, within BBR_RS13225 at 742.539 kb on - strand, within BBR_RS13225 at 742.625 kb on - strand, within BBR_RS13225 at 742.671 kb on - strand, within BBR_RS13225 at 742.685 kb on + strand, within BBR_RS13225 at 742.693 kb on - strand, within BBR_RS13225 at 742.693 kb on - strand, within BBR_RS13225 at 742.698 kb on - strand, within BBR_RS13225 at 742.749 kb on - strand, within BBR_RS13225 at 742.762 kb on + strand, within BBR_RS13225 at 742.832 kb on + strand, within BBR_RS13225 at 742.832 kb on + strand, within BBR_RS13225 at 742.832 kb on + strand, within BBR_RS13225 at 742.850 kb on + strand, within BBR_RS13225 at 742.856 kb on + strand, within BBR_RS13225 at 742.864 kb on - strand, within BBR_RS13225 at 742.869 kb on + strand, within BBR_RS13225 at 742.872 kb on - strand, within BBR_RS13225 at 742.877 kb on - strand, within BBR_RS13225 at 742.894 kb on - strand, within BBR_RS13225 at 742.894 kb on - strand, within BBR_RS13225 at 742.894 kb on - strand, within BBR_RS13225 at 742.940 kb on + strand, within BBR_RS13225 at 742.947 kb on - strand, within BBR_RS13225 at 742.947 kb on - strand, within BBR_RS13225 at 742.948 kb on - strand, within BBR_RS13225 at 742.948 kb on - strand, within BBR_RS13225 at 742.950 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Ciprofloxacin 0.1 mM remove 740,595 + BBR_RS13210 0.62 +1.9 740,659 + BBR_RS13210 0.67 -0.3 740,672 - BBR_RS13210 0.69 +0.8 740,677 + BBR_RS13210 0.69 -2.4 740,753 - BBR_RS13210 0.75 +2.5 740,770 - BBR_RS13210 0.77 -0.3 740,815 - BBR_RS13210 0.81 -1.4 740,878 - BBR_RS13210 0.86 -0.9 741,000 + +0.4 741,072 - BBR_RS13215 0.12 -1.1 741,111 - BBR_RS13215 0.29 -0.4 741,226 + BBR_RS13215 0.81 -1.4 741,276 + -1.1 741,278 + +1.2 741,278 + -1.2 741,284 - -1.4 741,286 - -1.6 741,478 + -1.9 741,478 + +1.5 741,486 - +0.7 741,486 - -0.6 741,493 - +1.6 741,500 + -0.8 741,500 + +0.2 741,618 + BBR_RS13220 0.13 +1.4 741,742 + BBR_RS13220 0.40 -0.4 741,750 - BBR_RS13220 0.41 -1.8 741,751 - BBR_RS13220 0.42 +1.0 741,823 + BBR_RS13220 0.57 +0.3 741,848 - BBR_RS13220 0.63 +0.4 741,850 - BBR_RS13220 0.63 -2.4 741,956 + BBR_RS13220 0.86 -1.0 741,963 + BBR_RS13220 0.88 -1.7 741,963 + BBR_RS13220 0.88 -1.9 741,963 + BBR_RS13220 0.88 +1.8 741,965 + BBR_RS13220 0.88 -1.0 741,988 - +0.8 741,990 - -0.7 741,990 - +1.4 742,006 - -0.8 742,023 - -0.5 742,041 - +1.8 742,072 + +1.3 742,096 - -1.9 742,115 + +1.4 742,115 + -1.6 742,133 - +1.3 742,137 + +1.0 742,151 - +0.4 742,158 + -1.6 742,174 - -1.8 742,190 - +3.2 742,205 + -2.7 742,237 - +2.2 742,246 + -2.4 742,248 + +1.9 742,254 - BBR_RS13225 0.10 -0.8 742,321 + BBR_RS13225 0.18 +0.3 742,321 + BBR_RS13225 0.18 -0.2 742,342 - BBR_RS13225 0.20 -1.2 742,375 + BBR_RS13225 0.24 +0.2 742,460 + BBR_RS13225 0.34 -2.8 742,466 - BBR_RS13225 0.34 +1.5 742,539 - BBR_RS13225 0.43 +1.0 742,625 - BBR_RS13225 0.53 +2.9 742,671 - BBR_RS13225 0.58 -0.4 742,685 + BBR_RS13225 0.60 -0.7 742,693 - BBR_RS13225 0.61 -2.2 742,693 - BBR_RS13225 0.61 -1.9 742,698 - BBR_RS13225 0.61 -1.5 742,749 - BBR_RS13225 0.67 -0.8 742,762 + BBR_RS13225 0.69 -0.3 742,832 + BBR_RS13225 0.77 -3.3 742,832 + BBR_RS13225 0.77 +1.5 742,832 + BBR_RS13225 0.77 -1.5 742,850 + BBR_RS13225 0.79 +2.6 742,856 + BBR_RS13225 0.79 -0.7 742,864 - BBR_RS13225 0.80 -0.6 742,869 + BBR_RS13225 0.81 -1.3 742,872 - BBR_RS13225 0.81 -2.8 742,877 - BBR_RS13225 0.82 +0.0 742,894 - BBR_RS13225 0.84 +0.5 742,894 - BBR_RS13225 0.84 -0.9 742,894 - BBR_RS13225 0.84 -0.9 742,940 + BBR_RS13225 0.89 +0.4 742,947 - BBR_RS13225 0.90 +0.5 742,947 - BBR_RS13225 0.90 +0.7 742,948 - BBR_RS13225 0.90 -0.3 742,948 - BBR_RS13225 0.90 -1.9 742,950 - +2.7
Or see this region's nucleotide sequence