Experiment: Recovery broth with Ciprofloxacin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS11805 and BBR_RS11810 are separated by 233 nucleotides BBR_RS11810 and BBR_RS11815 are separated by 85 nucleotides BBR_RS11815 and BBR_RS11820 are separated by 246 nucleotides
BBR_RS11805: BBR_RS11805 - hypothetical protein, at 407,333 to 407,911
_RS11805
BBR_RS11810: BBR_RS11810 - inorganic pyrophosphatase, at 408,145 to 408,639
_RS11810
BBR_RS11815: BBR_RS11815 - DUF3416 domain-containing protein, at 408,725 to 410,953
_RS11815
BBR_RS11820: BBR_RS11820 - hypothetical protein, at 411,200 to 413,134
_RS11820
Position (kb) 408
409
410
411 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 407.736 kb on + strand, within BBR_RS11805 at 407.781 kb on - strand, within BBR_RS11805 at 407.790 kb on + strand, within BBR_RS11805 at 407.809 kb on - strand, within BBR_RS11805 at 407.809 kb on - strand, within BBR_RS11805 at 407.826 kb on + strand, within BBR_RS11805 at 407.826 kb on + strand, within BBR_RS11805 at 407.832 kb on - strand, within BBR_RS11805 at 407.833 kb on - strand, within BBR_RS11805 at 407.848 kb on - strand, within BBR_RS11805 at 407.879 kb on + strand at 407.882 kb on + strand at 407.887 kb on - strand at 407.889 kb on + strand at 407.896 kb on - strand at 407.907 kb on + strand at 407.939 kb on + strand at 407.939 kb on + strand at 407.954 kb on - strand at 407.964 kb on - strand at 407.970 kb on - strand at 407.977 kb on + strand at 408.000 kb on + strand at 408.008 kb on + strand at 408.008 kb on - strand at 408.008 kb on - strand at 408.008 kb on - strand at 408.010 kb on - strand at 408.016 kb on - strand at 408.044 kb on - strand at 408.052 kb on + strand at 408.070 kb on + strand at 408.072 kb on + strand at 408.080 kb on - strand at 408.140 kb on - strand at 408.670 kb on - strand at 408.670 kb on - strand at 408.670 kb on - strand at 408.672 kb on - strand at 408.673 kb on + strand at 408.676 kb on + strand at 408.676 kb on + strand at 408.676 kb on + strand at 408.676 kb on + strand at 408.676 kb on + strand at 408.676 kb on + strand at 408.676 kb on + strand at 408.678 kb on + strand at 408.678 kb on + strand at 408.680 kb on + strand at 408.684 kb on - strand at 408.684 kb on - strand at 408.684 kb on - strand at 408.684 kb on - strand at 408.684 kb on - strand at 408.684 kb on - strand at 408.684 kb on - strand at 408.684 kb on - strand at 408.686 kb on - strand at 408.686 kb on - strand at 408.686 kb on - strand at 408.693 kb on + strand at 408.699 kb on + strand at 408.703 kb on - strand at 408.707 kb on - strand at 408.707 kb on - strand at 408.707 kb on - strand at 408.707 kb on - strand at 408.707 kb on - strand at 408.707 kb on - strand at 408.707 kb on - strand at 408.707 kb on - strand at 408.716 kb on + strand at 408.790 kb on - strand at 409.225 kb on - strand, within BBR_RS11815 at 410.818 kb on - strand at 410.849 kb on - strand at 410.858 kb on - strand at 410.888 kb on - strand at 410.903 kb on - strand at 410.903 kb on - strand at 410.936 kb on + strand at 410.936 kb on + strand at 410.936 kb on + strand at 410.944 kb on - strand at 411.061 kb on - strand at 411.061 kb on - strand at 411.064 kb on - strand at 411.091 kb on + strand at 411.092 kb on + strand at 411.097 kb on + strand at 411.097 kb on + strand at 411.105 kb on - strand at 411.105 kb on - strand at 411.119 kb on + strand at 411.119 kb on + strand at 411.119 kb on + strand at 411.119 kb on + strand at 411.127 kb on - strand at 411.129 kb on + strand at 411.154 kb on + strand at 411.176 kb on - strand at 411.204 kb on - strand at 411.210 kb on - strand at 411.223 kb on + strand at 411.228 kb on + strand at 411.228 kb on + strand at 411.228 kb on + strand at 411.233 kb on + strand at 411.236 kb on - strand at 411.236 kb on - strand at 411.236 kb on - strand at 411.236 kb on - strand at 411.236 kb on - strand at 411.236 kb on - strand at 411.236 kb on - strand at 411.241 kb on - strand at 411.251 kb on + strand at 411.275 kb on + strand at 411.283 kb on - strand at 411.283 kb on - strand at 411.288 kb on - strand at 411.288 kb on - strand at 411.288 kb on - strand at 411.290 kb on + strand at 411.290 kb on + strand at 411.295 kb on + strand at 411.305 kb on - strand at 411.309 kb on + strand at 411.332 kb on + strand at 411.332 kb on - strand at 411.340 kb on - strand at 411.364 kb on + strand at 411.372 kb on + strand at 411.380 kb on + strand at 411.388 kb on - strand at 411.388 kb on - strand at 411.390 kb on + strand at 411.398 kb on - strand, within BBR_RS11820 at 411.424 kb on + strand, within BBR_RS11820 at 411.426 kb on + strand, within BBR_RS11820 at 411.432 kb on - strand, within BBR_RS11820 at 411.436 kb on + strand, within BBR_RS11820 at 411.444 kb on - strand, within BBR_RS11820 at 411.444 kb on - strand, within BBR_RS11820 at 411.508 kb on + strand, within BBR_RS11820 at 411.510 kb on + strand, within BBR_RS11820 at 411.516 kb on - strand, within BBR_RS11820 at 411.516 kb on - strand, within BBR_RS11820 at 411.533 kb on + strand, within BBR_RS11820 at 411.587 kb on + strand, within BBR_RS11820 at 411.593 kb on - strand, within BBR_RS11820 at 411.598 kb on + strand, within BBR_RS11820 at 411.619 kb on + strand, within BBR_RS11820 at 411.641 kb on + strand, within BBR_RS11820 at 411.641 kb on + strand, within BBR_RS11820 at 411.644 kb on - strand, within BBR_RS11820 at 411.649 kb on - strand, within BBR_RS11820 at 411.649 kb on - strand, within BBR_RS11820 at 411.651 kb on + strand, within BBR_RS11820 at 411.654 kb on - strand, within BBR_RS11820 at 411.684 kb on + strand, within BBR_RS11820 at 411.686 kb on + strand, within BBR_RS11820 at 411.705 kb on + strand, within BBR_RS11820 at 411.738 kb on + strand, within BBR_RS11820 at 411.738 kb on + strand, within BBR_RS11820 at 411.745 kb on + strand, within BBR_RS11820 at 411.746 kb on + strand, within BBR_RS11820 at 411.748 kb on + strand, within BBR_RS11820 at 411.748 kb on + strand, within BBR_RS11820 at 411.754 kb on - strand, within BBR_RS11820 at 411.756 kb on - strand, within BBR_RS11820 at 411.756 kb on - strand, within BBR_RS11820 at 411.763 kb on + strand, within BBR_RS11820 at 411.771 kb on - strand, within BBR_RS11820 at 411.778 kb on + strand, within BBR_RS11820 at 411.783 kb on + strand, within BBR_RS11820 at 411.783 kb on + strand, within BBR_RS11820 at 411.789 kb on + strand, within BBR_RS11820 at 411.789 kb on + strand, within BBR_RS11820 at 411.797 kb on - strand, within BBR_RS11820 at 411.832 kb on + strand, within BBR_RS11820 at 411.832 kb on + strand, within BBR_RS11820 at 411.842 kb on + strand, within BBR_RS11820 at 411.842 kb on + strand, within BBR_RS11820 at 411.857 kb on + strand, within BBR_RS11820 at 411.857 kb on + strand, within BBR_RS11820 at 411.862 kb on + strand, within BBR_RS11820 at 411.865 kb on - strand, within BBR_RS11820 at 411.865 kb on - strand, within BBR_RS11820 at 411.934 kb on - strand, within BBR_RS11820 at 411.936 kb on + strand, within BBR_RS11820 at 411.938 kb on + strand, within BBR_RS11820 at 411.953 kb on - strand, within BBR_RS11820 at 411.953 kb on - strand, within BBR_RS11820 at 411.953 kb on - strand, within BBR_RS11820 at 411.953 kb on - strand, within BBR_RS11820
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Ciprofloxacin 0.1 mM remove 407,736 + BBR_RS11805 0.70 +0.7 407,781 - BBR_RS11805 0.77 -0.2 407,790 + BBR_RS11805 0.79 -2.7 407,809 - BBR_RS11805 0.82 +0.0 407,809 - BBR_RS11805 0.82 +1.9 407,826 + BBR_RS11805 0.85 -0.4 407,826 + BBR_RS11805 0.85 -0.8 407,832 - BBR_RS11805 0.86 -1.2 407,833 - BBR_RS11805 0.86 +2.0 407,848 - BBR_RS11805 0.89 -1.5 407,879 + +2.0 407,882 + +2.3 407,887 - -2.0 407,889 + +0.3 407,896 - +1.6 407,907 + +1.6 407,939 + -1.1 407,939 + +2.8 407,954 - -0.4 407,964 - -0.0 407,970 - +0.9 407,977 + +1.7 408,000 + -0.9 408,008 + -1.8 408,008 - +1.0 408,008 - +1.3 408,008 - -1.0 408,010 - +1.5 408,016 - +1.5 408,044 - -1.2 408,052 + -2.1 408,070 + -1.7 408,072 + -2.0 408,080 - -1.3 408,140 - +0.1 408,670 - -1.3 408,670 - +2.3 408,670 - -0.7 408,672 - -1.6 408,673 + -0.2 408,676 + +1.6 408,676 + -2.6 408,676 + -1.3 408,676 + -2.4 408,676 + +0.4 408,676 + -1.6 408,676 + -2.6 408,678 + +1.2 408,678 + +1.8 408,680 + -1.4 408,684 - -2.9 408,684 - +0.0 408,684 - -0.9 408,684 - +1.2 408,684 - +0.6 408,684 - -2.0 408,684 - +0.3 408,684 - +0.2 408,686 - -1.0 408,686 - +3.6 408,686 - +2.8 408,693 + +0.3 408,699 + +1.6 408,703 - -1.2 408,707 - +3.3 408,707 - +0.1 408,707 - -0.6 408,707 - +0.6 408,707 - +1.7 408,707 - -3.2 408,707 - -2.0 408,707 - +1.6 408,716 + -1.9 408,790 - +0.7 409,225 - BBR_RS11815 0.22 +3.4 410,818 - +0.2 410,849 - +0.6 410,858 - +1.8 410,888 - +0.4 410,903 - -1.9 410,903 - +0.7 410,936 + +0.3 410,936 + -1.6 410,936 + +0.1 410,944 - -1.5 411,061 - +2.3 411,061 - +2.2 411,064 - +2.3 411,091 + -0.9 411,092 + -0.4 411,097 + -0.6 411,097 + -0.9 411,105 - -0.2 411,105 - -1.6 411,119 + +0.1 411,119 + -1.6 411,119 + -0.5 411,119 + +0.6 411,127 - +1.2 411,129 + -2.1 411,154 + -1.7 411,176 - +3.2 411,204 - -0.6 411,210 - +1.5 411,223 + +0.2 411,228 + +0.2 411,228 + -0.3 411,228 + +0.4 411,233 + -1.1 411,236 - -2.1 411,236 - -0.5 411,236 - +0.7 411,236 - -2.1 411,236 - -0.1 411,236 - +0.2 411,236 - +1.0 411,241 - -2.1 411,251 + -0.4 411,275 + +2.0 411,283 - +1.8 411,283 - -2.4 411,288 - +2.6 411,288 - +1.0 411,288 - +0.8 411,290 + +0.6 411,290 + +0.8 411,295 + +2.2 411,305 - +1.2 411,309 + -1.4 411,332 + +1.7 411,332 - +1.0 411,340 - +1.1 411,364 + -1.5 411,372 + +0.2 411,380 + +0.4 411,388 - +1.1 411,388 - +1.1 411,390 + -1.9 411,398 - BBR_RS11820 0.10 -1.6 411,424 + BBR_RS11820 0.12 +0.2 411,426 + BBR_RS11820 0.12 +2.0 411,432 - BBR_RS11820 0.12 +0.8 411,436 + BBR_RS11820 0.12 -1.1 411,444 - BBR_RS11820 0.13 +0.8 411,444 - BBR_RS11820 0.13 +0.3 411,508 + BBR_RS11820 0.16 +1.3 411,510 + BBR_RS11820 0.16 -2.3 411,516 - BBR_RS11820 0.16 +0.0 411,516 - BBR_RS11820 0.16 +1.2 411,533 + BBR_RS11820 0.17 -0.3 411,587 + BBR_RS11820 0.20 -1.8 411,593 - BBR_RS11820 0.20 +0.5 411,598 + BBR_RS11820 0.21 +0.9 411,619 + BBR_RS11820 0.22 -2.0 411,641 + BBR_RS11820 0.23 +1.2 411,641 + BBR_RS11820 0.23 -2.3 411,644 - BBR_RS11820 0.23 +0.5 411,649 - BBR_RS11820 0.23 -1.3 411,649 - BBR_RS11820 0.23 +1.4 411,651 + BBR_RS11820 0.23 +0.6 411,654 - BBR_RS11820 0.23 -0.8 411,684 + BBR_RS11820 0.25 +0.8 411,686 + BBR_RS11820 0.25 +0.4 411,705 + BBR_RS11820 0.26 +1.3 411,738 + BBR_RS11820 0.28 -1.0 411,738 + BBR_RS11820 0.28 -0.6 411,745 + BBR_RS11820 0.28 -3.1 411,746 + BBR_RS11820 0.28 +1.7 411,748 + BBR_RS11820 0.28 -0.7 411,748 + BBR_RS11820 0.28 +1.7 411,754 - BBR_RS11820 0.29 -0.8 411,756 - BBR_RS11820 0.29 +2.5 411,756 - BBR_RS11820 0.29 +0.9 411,763 + BBR_RS11820 0.29 -1.9 411,771 - BBR_RS11820 0.30 -1.8 411,778 + BBR_RS11820 0.30 +1.4 411,783 + BBR_RS11820 0.30 -0.9 411,783 + BBR_RS11820 0.30 -1.5 411,789 + BBR_RS11820 0.30 +0.2 411,789 + BBR_RS11820 0.30 -1.0 411,797 - BBR_RS11820 0.31 -1.3 411,832 + BBR_RS11820 0.33 -0.9 411,832 + BBR_RS11820 0.33 -0.4 411,842 + BBR_RS11820 0.33 -0.4 411,842 + BBR_RS11820 0.33 -2.1 411,857 + BBR_RS11820 0.34 -0.9 411,857 + BBR_RS11820 0.34 -0.9 411,862 + BBR_RS11820 0.34 -0.2 411,865 - BBR_RS11820 0.34 +1.0 411,865 - BBR_RS11820 0.34 -0.4 411,934 - BBR_RS11820 0.38 -2.1 411,936 + BBR_RS11820 0.38 -0.4 411,938 + BBR_RS11820 0.38 -0.9 411,953 - BBR_RS11820 0.39 -1.2 411,953 - BBR_RS11820 0.39 +1.0 411,953 - BBR_RS11820 0.39 -0.4 411,953 - BBR_RS11820 0.39 +0.5
Or see this region's nucleotide sequence