Experiment: MoYLS4 with electrocompetent cells (sucrose treatment)+shock
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVUA0131 and DVUA0132 are separated by 2 nucleotides DVUA0132 and DVUA0133 are separated by 1 nucleotides DVUA0133 and cas1 overlap by 17 nucleotides
DVUA0131: DVUA0131 - CRISPR-associated protein, CT1133 family (TIGR), at 171,056 to 172,894
DVUA0131
DVUA0132: DVUA0132 - CRISPR-associated protein, TM1801 family (TIGR), at 172,897 to 173,769
DVUA0132
DVUA0133: DVUA0133 - CRISPR-associated protein Cas4 (TIGR), at 173,771 to 174,409
DVUA0133
DVUA0134: cas1 - CRISPR-associated protein Cas1 (TIGR), at 174,393 to 175,424
cas1
Position (kb) 172
173
174 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 171.930 kb on - strand, within DVUA0131 at 171.991 kb on + strand, within DVUA0131 at 172.079 kb on - strand, within DVUA0131 at 172.089 kb on + strand, within DVUA0131 at 172.118 kb on - strand, within DVUA0131 at 172.184 kb on + strand, within DVUA0131 at 172.189 kb on + strand, within DVUA0131 at 172.294 kb on + strand, within DVUA0131 at 172.336 kb on + strand, within DVUA0131 at 172.336 kb on + strand, within DVUA0131 at 172.344 kb on - strand, within DVUA0131 at 172.407 kb on - strand, within DVUA0131 at 172.414 kb on + strand, within DVUA0131 at 172.422 kb on - strand, within DVUA0131 at 172.556 kb on - strand, within DVUA0131 at 172.575 kb on - strand, within DVUA0131 at 172.625 kb on + strand, within DVUA0131 at 172.831 kb on + strand at 172.848 kb on - strand at 172.863 kb on - strand at 173.031 kb on - strand, within DVUA0132 at 173.086 kb on - strand, within DVUA0132 at 173.139 kb on + strand, within DVUA0132 at 173.232 kb on + strand, within DVUA0132 at 173.268 kb on - strand, within DVUA0132 at 173.268 kb on - strand, within DVUA0132 at 173.274 kb on + strand, within DVUA0132 at 173.274 kb on + strand, within DVUA0132 at 173.282 kb on - strand, within DVUA0132 at 173.490 kb on + strand, within DVUA0132 at 173.523 kb on + strand, within DVUA0132 at 173.523 kb on + strand, within DVUA0132 at 173.523 kb on + strand, within DVUA0132 at 173.523 kb on + strand, within DVUA0132 at 173.531 kb on - strand, within DVUA0132 at 173.531 kb on - strand, within DVUA0132 at 173.611 kb on + strand, within DVUA0132 at 173.672 kb on - strand, within DVUA0132 at 173.723 kb on + strand at 173.736 kb on - strand at 173.744 kb on + strand at 173.751 kb on + strand at 173.816 kb on + strand at 173.821 kb on + strand at 173.821 kb on + strand at 173.871 kb on - strand, within DVUA0133 at 173.876 kb on + strand, within DVUA0133 at 173.889 kb on - strand, within DVUA0133 at 173.889 kb on - strand, within DVUA0133 at 173.924 kb on + strand, within DVUA0133 at 173.930 kb on - strand, within DVUA0133 at 173.964 kb on + strand, within DVUA0133 at 173.964 kb on + strand, within DVUA0133 at 173.993 kb on - strand, within DVUA0133 at 174.003 kb on - strand, within DVUA0133 at 174.014 kb on + strand, within DVUA0133 at 174.033 kb on + strand, within DVUA0133 at 174.040 kb on + strand, within DVUA0133 at 174.095 kb on + strand, within DVUA0133 at 174.163 kb on + strand, within DVUA0133 at 174.325 kb on - strand, within DVUA0133 at 174.352 kb on + strand at 174.367 kb on + strand at 174.367 kb on - strand at 174.404 kb on + strand at 174.412 kb on - strand at 174.442 kb on + strand at 174.450 kb on - strand at 174.473 kb on + strand at 174.548 kb on - strand, within cas1 at 174.558 kb on - strand, within cas1 at 174.630 kb on + strand, within cas1 at 174.660 kb on - strand, within cas1 at 174.677 kb on + strand, within cas1 at 174.711 kb on - strand, within cas1
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with electrocompetent cells (sucrose treatment)+shock remove 171,930 - DVUA0131 0.48 -1.1 171,991 + DVUA0131 0.51 -0.3 172,079 - DVUA0131 0.56 +0.5 172,089 + DVUA0131 0.56 +0.9 172,118 - DVUA0131 0.58 -0.4 172,184 + DVUA0131 0.61 -0.2 172,189 + DVUA0131 0.62 -0.6 172,294 + DVUA0131 0.67 +0.8 172,336 + DVUA0131 0.70 -0.8 172,336 + DVUA0131 0.70 +0.1 172,344 - DVUA0131 0.70 -0.2 172,407 - DVUA0131 0.73 -1.2 172,414 + DVUA0131 0.74 -1.2 172,422 - DVUA0131 0.74 +0.9 172,556 - DVUA0131 0.82 +0.1 172,575 - DVUA0131 0.83 +0.3 172,625 + DVUA0131 0.85 -2.8 172,831 + -0.3 172,848 - -0.2 172,863 - -0.1 173,031 - DVUA0132 0.15 -0.9 173,086 - DVUA0132 0.22 -0.2 173,139 + DVUA0132 0.28 +1.9 173,232 + DVUA0132 0.38 -0.5 173,268 - DVUA0132 0.42 -1.8 173,268 - DVUA0132 0.42 -1.3 173,274 + DVUA0132 0.43 -0.2 173,274 + DVUA0132 0.43 +0.1 173,282 - DVUA0132 0.44 -0.4 173,490 + DVUA0132 0.68 -1.8 173,523 + DVUA0132 0.72 -1.0 173,523 + DVUA0132 0.72 -0.3 173,523 + DVUA0132 0.72 -0.4 173,523 + DVUA0132 0.72 -0.1 173,531 - DVUA0132 0.73 -0.3 173,531 - DVUA0132 0.73 -0.2 173,611 + DVUA0132 0.82 -0.7 173,672 - DVUA0132 0.89 -0.3 173,723 + -0.2 173,736 - -0.2 173,744 + -0.4 173,751 + -0.3 173,816 + -0.2 173,821 + -0.1 173,821 + +1.9 173,871 - DVUA0133 0.16 -1.7 173,876 + DVUA0133 0.16 +0.1 173,889 - DVUA0133 0.18 -0.7 173,889 - DVUA0133 0.18 -0.5 173,924 + DVUA0133 0.24 -0.5 173,930 - DVUA0133 0.25 -0.5 173,964 + DVUA0133 0.30 +0.2 173,964 + DVUA0133 0.30 -1.1 173,993 - DVUA0133 0.35 +0.3 174,003 - DVUA0133 0.36 -0.2 174,014 + DVUA0133 0.38 -0.1 174,033 + DVUA0133 0.41 -1.0 174,040 + DVUA0133 0.42 -0.9 174,095 + DVUA0133 0.51 -0.2 174,163 + DVUA0133 0.61 -0.6 174,325 - DVUA0133 0.87 -1.9 174,352 + +0.3 174,367 + +0.9 174,367 - -0.0 174,404 + -0.1 174,412 - -0.1 174,442 + +0.2 174,450 - -0.6 174,473 + -0.1 174,548 - cas1 DVUA0134 0.15 +0.5 174,558 - cas1 DVUA0134 0.16 -1.1 174,630 + cas1 DVUA0134 0.23 +0.6 174,660 - cas1 DVUA0134 0.26 +0.0 174,677 + cas1 DVUA0134 0.28 +1.5 174,711 - cas1 DVUA0134 0.31 -0.2
Or see this region's nucleotide sequence