Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS20095 and HSERO_RS20100 overlap by 4 nucleotides HSERO_RS20100 and HSERO_RS20105 are separated by 44 nucleotides HSERO_RS20105 and HSERO_RS20110 are separated by 93 nucleotides
HSERO_RS20095: HSERO_RS20095 - ABC transporter permease, at 4,610,550 to 4,611,500
_RS20095
HSERO_RS20100: HSERO_RS20100 - ABC transporter permease, at 4,611,497 to 4,612,444
_RS20100
HSERO_RS20105: HSERO_RS20105 - ABC transporter ATP-binding protein, at 4,612,489 to 4,613,997
_RS20105
HSERO_RS20110: HSERO_RS20110 - ABC transporter substrate-binding protein, at 4,614,091 to 4,615,158
_RS20110
Position (kb)
4612
4613
4614 Strain fitness (log2 ratio)
-1
0
1
2 at 4611.613 kb on - strand, within HSERO_RS20100 at 4611.637 kb on + strand, within HSERO_RS20100 at 4611.645 kb on - strand, within HSERO_RS20100 at 4611.771 kb on - strand, within HSERO_RS20100 at 4611.931 kb on + strand, within HSERO_RS20100 at 4611.931 kb on + strand, within HSERO_RS20100 at 4611.945 kb on + strand, within HSERO_RS20100 at 4612.260 kb on - strand, within HSERO_RS20100 at 4612.303 kb on - strand, within HSERO_RS20100 at 4612.338 kb on - strand, within HSERO_RS20100 at 4612.398 kb on - strand at 4612.418 kb on + strand at 4612.502 kb on + strand at 4612.510 kb on - strand at 4612.590 kb on - strand at 4612.596 kb on + strand at 4612.604 kb on - strand at 4612.604 kb on - strand at 4612.748 kb on - strand, within HSERO_RS20105 at 4612.802 kb on - strand, within HSERO_RS20105 at 4612.865 kb on - strand, within HSERO_RS20105 at 4612.984 kb on + strand, within HSERO_RS20105 at 4613.182 kb on + strand, within HSERO_RS20105 at 4613.212 kb on - strand, within HSERO_RS20105 at 4613.249 kb on + strand, within HSERO_RS20105 at 4613.350 kb on + strand, within HSERO_RS20105 at 4613.573 kb on + strand, within HSERO_RS20105 at 4613.660 kb on - strand, within HSERO_RS20105 at 4613.812 kb on + strand, within HSERO_RS20105 at 4613.820 kb on - strand, within HSERO_RS20105 at 4613.836 kb on + strand, within HSERO_RS20105 at 4613.882 kb on - strand at 4613.952 kb on + strand at 4613.962 kb on + strand at 4614.007 kb on + strand at 4614.148 kb on - strand at 4614.178 kb on - strand at 4614.178 kb on - strand at 4614.195 kb on + strand at 4614.206 kb on + strand, within HSERO_RS20110 at 4614.207 kb on + strand, within HSERO_RS20110 at 4614.275 kb on + strand, within HSERO_RS20110 at 4614.340 kb on + strand, within HSERO_RS20110 at 4614.478 kb on - strand, within HSERO_RS20110 at 4614.518 kb on + strand, within HSERO_RS20110 at 4614.762 kb on + strand, within HSERO_RS20110 at 4614.982 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 4,611,613 - HSERO_RS20100 0.12 -0.3 4,611,637 + HSERO_RS20100 0.15 +0.7 4,611,645 - HSERO_RS20100 0.16 -0.6 4,611,771 - HSERO_RS20100 0.29 +0.7 4,611,931 + HSERO_RS20100 0.46 -0.5 4,611,931 + HSERO_RS20100 0.46 +0.6 4,611,945 + HSERO_RS20100 0.47 +0.0 4,612,260 - HSERO_RS20100 0.80 -0.2 4,612,303 - HSERO_RS20100 0.85 +0.2 4,612,338 - HSERO_RS20100 0.89 +1.0 4,612,398 - +0.2 4,612,418 + -0.2 4,612,502 + +0.0 4,612,510 - +0.0 4,612,590 - -0.1 4,612,596 + +0.1 4,612,604 - -0.1 4,612,604 - -0.5 4,612,748 - HSERO_RS20105 0.17 +0.4 4,612,802 - HSERO_RS20105 0.21 -0.3 4,612,865 - HSERO_RS20105 0.25 +2.2 4,612,984 + HSERO_RS20105 0.33 +0.8 4,613,182 + HSERO_RS20105 0.46 -0.1 4,613,212 - HSERO_RS20105 0.48 +0.5 4,613,249 + HSERO_RS20105 0.50 -0.3 4,613,350 + HSERO_RS20105 0.57 +1.1 4,613,573 + HSERO_RS20105 0.72 +1.3 4,613,660 - HSERO_RS20105 0.78 +0.0 4,613,812 + HSERO_RS20105 0.88 +1.5 4,613,820 - HSERO_RS20105 0.88 +0.3 4,613,836 + HSERO_RS20105 0.89 +1.2 4,613,882 - -0.1 4,613,952 + -0.5 4,613,962 + +0.0 4,614,007 + +0.1 4,614,148 - -0.3 4,614,178 - +0.2 4,614,178 - -0.2 4,614,195 + -1.4 4,614,206 + HSERO_RS20110 0.11 -1.0 4,614,207 + HSERO_RS20110 0.11 +0.1 4,614,275 + HSERO_RS20110 0.17 -0.1 4,614,340 + HSERO_RS20110 0.23 -0.1 4,614,478 - HSERO_RS20110 0.36 -0.4 4,614,518 + HSERO_RS20110 0.40 +0.9 4,614,762 + HSERO_RS20110 0.63 -0.2 4,614,982 + +0.5
Or see this region's nucleotide sequence