Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS06485 and HSERO_RS06490 are separated by 153 nucleotides HSERO_RS06490 and HSERO_RS06495 are separated by 65 nucleotides HSERO_RS06495 and HSERO_RS06500 overlap by 22 nucleotides
HSERO_RS06485: HSERO_RS06485 - filamentous hemagglutinin protein, at 1,465,642 to 1,473,618
_RS06485
HSERO_RS06490: HSERO_RS06490 - hypothetical protein, at 1,473,772 to 1,474,428
_RS06490
HSERO_RS06495: HSERO_RS06495 - nuclease, at 1,474,494 to 1,476,149
_RS06495
HSERO_RS06500: HSERO_RS06500 - ATP-dependent DNA helicase, at 1,476,128 to 1,478,401
_RS06500
Position (kb)
1474
1475
1476
1477 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5
6 at 1473.549 kb on - strand at 1473.555 kb on + strand at 1473.563 kb on - strand at 1473.563 kb on - strand at 1473.595 kb on - strand at 1473.631 kb on + strand at 1473.652 kb on - strand at 1474.386 kb on - strand at 1474.509 kb on - strand at 1474.539 kb on + strand at 1474.554 kb on + strand at 1474.554 kb on + strand at 1474.595 kb on + strand at 1474.723 kb on - strand, within HSERO_RS06495 at 1474.898 kb on - strand, within HSERO_RS06495 at 1475.097 kb on - strand, within HSERO_RS06495 at 1475.197 kb on - strand, within HSERO_RS06495 at 1475.278 kb on - strand, within HSERO_RS06495 at 1475.323 kb on + strand, within HSERO_RS06495 at 1475.395 kb on - strand, within HSERO_RS06495 at 1475.395 kb on - strand, within HSERO_RS06495 at 1475.395 kb on - strand, within HSERO_RS06495 at 1475.399 kb on + strand, within HSERO_RS06495 at 1475.399 kb on + strand, within HSERO_RS06495 at 1475.407 kb on - strand, within HSERO_RS06495 at 1475.594 kb on - strand, within HSERO_RS06495 at 1475.732 kb on - strand, within HSERO_RS06495 at 1475.784 kb on - strand, within HSERO_RS06495 at 1475.906 kb on - strand, within HSERO_RS06495 at 1476.084 kb on - strand at 1476.138 kb on - strand at 1476.236 kb on - strand at 1476.241 kb on - strand at 1476.321 kb on - strand at 1476.436 kb on - strand at 1476.468 kb on + strand, within HSERO_RS06500 at 1476.494 kb on + strand at 1476.496 kb on + strand, within HSERO_RS06500 at 1476.536 kb on - strand, within HSERO_RS06500 at 1476.538 kb on + strand, within HSERO_RS06500 at 1476.538 kb on + strand, within HSERO_RS06500 at 1476.546 kb on - strand, within HSERO_RS06500 at 1476.546 kb on - strand, within HSERO_RS06500 at 1476.621 kb on - strand, within HSERO_RS06500 at 1476.846 kb on - strand, within HSERO_RS06500 at 1476.880 kb on - strand, within HSERO_RS06500 at 1477.080 kb on + strand, within HSERO_RS06500 at 1477.097 kb on - strand, within HSERO_RS06500 at 1477.104 kb on + strand, within HSERO_RS06500 at 1477.112 kb on - strand, within HSERO_RS06500 at 1477.122 kb on - strand, within HSERO_RS06500
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 1,473,549 - -1.4 1,473,555 + -3.0 1,473,563 - -3.8 1,473,563 - -5.0 1,473,595 - -2.7 1,473,631 + -2.4 1,473,652 - +2.3 1,474,386 - -0.5 1,474,509 - +1.3 1,474,539 + +0.1 1,474,554 + +0.1 1,474,554 + -0.4 1,474,595 + +0.1 1,474,723 - HSERO_RS06495 0.14 -1.3 1,474,898 - HSERO_RS06495 0.24 +0.3 1,475,097 - HSERO_RS06495 0.36 +0.5 1,475,197 - HSERO_RS06495 0.42 +1.4 1,475,278 - HSERO_RS06495 0.47 -0.5 1,475,323 + HSERO_RS06495 0.50 +1.0 1,475,395 - HSERO_RS06495 0.54 +0.3 1,475,395 - HSERO_RS06495 0.54 +0.3 1,475,395 - HSERO_RS06495 0.54 -1.6 1,475,399 + HSERO_RS06495 0.55 +0.0 1,475,399 + HSERO_RS06495 0.55 +2.0 1,475,407 - HSERO_RS06495 0.55 +0.1 1,475,594 - HSERO_RS06495 0.66 +0.3 1,475,732 - HSERO_RS06495 0.75 +0.2 1,475,784 - HSERO_RS06495 0.78 +0.8 1,475,906 - HSERO_RS06495 0.85 -0.6 1,476,084 - -0.1 1,476,138 - +0.4 1,476,236 - +0.7 1,476,241 - -1.3 1,476,321 - -0.5 1,476,436 - -1.3 1,476,468 + HSERO_RS06500 0.15 -0.3 1,476,494 + +5.9 1,476,496 + HSERO_RS06500 0.16 +0.3 1,476,536 - HSERO_RS06500 0.18 -0.1 1,476,538 + HSERO_RS06500 0.18 -0.1 1,476,538 + HSERO_RS06500 0.18 +0.2 1,476,546 - HSERO_RS06500 0.18 +0.9 1,476,546 - HSERO_RS06500 0.18 -0.8 1,476,621 - HSERO_RS06500 0.22 +0.2 1,476,846 - HSERO_RS06500 0.32 +0.8 1,476,880 - HSERO_RS06500 0.33 -2.1 1,477,080 + HSERO_RS06500 0.42 +0.3 1,477,097 - HSERO_RS06500 0.43 +0.6 1,477,104 + HSERO_RS06500 0.43 -0.1 1,477,112 - HSERO_RS06500 0.43 +0.8 1,477,122 - HSERO_RS06500 0.44 -0.5
Or see this region's nucleotide sequence