Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS21745

Experiment: RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.5 x; and 2,2'-Dipyridyl 50 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS21740 and BWI76_RS21745 are separated by 185 nucleotidesBWI76_RS21745 and BWI76_RS21750 are separated by 141 nucleotides BWI76_RS21740: BWI76_RS21740 - transcriptional regulator, LysR family protein, at 4,543,871 to 4,544,764 _RS21740 BWI76_RS21745: BWI76_RS21745 - LysR family transcriptional regulator, at 4,544,950 to 4,545,837 _RS21745 BWI76_RS21750: BWI76_RS21750 - amidohydrolase family protein, at 4,545,979 to 4,547,376 _RS21750 Position (kb) 4544 4545 4546Strain fitness (log2 ratio) -2 -1 0 1at 4543.956 kb on - strandat 4544.193 kb on - strand, within BWI76_RS21740at 4544.230 kb on + strand, within BWI76_RS21740at 4544.378 kb on - strand, within BWI76_RS21740at 4544.485 kb on + strand, within BWI76_RS21740at 4544.696 kb on - strandat 4544.702 kb on - strandat 4544.755 kb on + strandat 4544.820 kb on + strandat 4544.828 kb on - strandat 4545.017 kb on + strandat 4545.025 kb on - strandat 4545.049 kb on - strand, within BWI76_RS21745at 4545.083 kb on - strand, within BWI76_RS21745at 4545.176 kb on + strand, within BWI76_RS21745at 4545.185 kb on + strand, within BWI76_RS21745at 4545.194 kb on + strand, within BWI76_RS21745at 4545.202 kb on - strand, within BWI76_RS21745at 4545.299 kb on - strand, within BWI76_RS21745at 4545.336 kb on + strandat 4545.381 kb on + strand, within BWI76_RS21745at 4545.418 kb on + strand, within BWI76_RS21745at 4545.418 kb on + strandat 4545.426 kb on - strand, within BWI76_RS21745at 4545.427 kb on + strand, within BWI76_RS21745at 4545.428 kb on + strand, within BWI76_RS21745at 4545.515 kb on - strandat 4545.646 kb on - strand, within BWI76_RS21745at 4545.660 kb on + strand, within BWI76_RS21745at 4545.668 kb on - strand, within BWI76_RS21745at 4545.668 kb on - strand, within BWI76_RS21745at 4545.673 kb on - strand, within BWI76_RS21745at 4545.673 kb on - strand, within BWI76_RS21745at 4545.702 kb on + strand, within BWI76_RS21745at 4545.821 kb on + strandat 4545.846 kb on + strandat 4545.848 kb on + strandat 4545.848 kb on + strandat 4546.041 kb on - strandat 4546.059 kb on - strandat 4546.067 kb on - strandat 4546.083 kb on + strandat 4546.111 kb on + strandat 4546.126 kb on + strand, within BWI76_RS21750at 4546.137 kb on + strand, within BWI76_RS21750at 4546.697 kb on + strand, within BWI76_RS21750at 4546.736 kb on + strand, within BWI76_RS21750at 4546.744 kb on - strand, within BWI76_RS21750at 4546.781 kb on + strand, within BWI76_RS21750at 4546.801 kb on - strand, within BWI76_RS21750

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.5 x; and 2,2'-Dipyridyl 50 uM
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4,543,956 - -0.6
4,544,193 - BWI76_RS21740 0.36 -1.2
4,544,230 + BWI76_RS21740 0.40 -0.8
4,544,378 - BWI76_RS21740 0.57 -0.6
4,544,485 + BWI76_RS21740 0.69 -0.5
4,544,696 - +0.2
4,544,702 - -0.1
4,544,755 + -0.2
4,544,820 + +0.0
4,544,828 - -0.5
4,545,017 + -0.5
4,545,025 - +0.0
4,545,049 - BWI76_RS21745 0.11 +1.1
4,545,083 - BWI76_RS21745 0.15 -0.1
4,545,176 + BWI76_RS21745 0.25 -1.7
4,545,185 + BWI76_RS21745 0.26 -0.2
4,545,194 + BWI76_RS21745 0.27 -0.2
4,545,202 - BWI76_RS21745 0.28 +0.7
4,545,299 - BWI76_RS21745 0.39 -0.1
4,545,336 + -0.0
4,545,381 + BWI76_RS21745 0.49 -0.3
4,545,418 + BWI76_RS21745 0.53 -0.7
4,545,418 + +0.8
4,545,426 - BWI76_RS21745 0.54 +0.2
4,545,427 + BWI76_RS21745 0.54 -0.3
4,545,428 + BWI76_RS21745 0.54 -0.4
4,545,515 - -0.0
4,545,646 - BWI76_RS21745 0.78 +0.8
4,545,660 + BWI76_RS21745 0.80 -2.1
4,545,668 - BWI76_RS21745 0.81 -0.2
4,545,668 - BWI76_RS21745 0.81 +0.2
4,545,673 - BWI76_RS21745 0.81 -0.0
4,545,673 - BWI76_RS21745 0.81 +0.4
4,545,702 + BWI76_RS21745 0.85 +0.1
4,545,821 + -0.5
4,545,846 + +1.1
4,545,848 + -0.4
4,545,848 + +0.1
4,546,041 - -0.5
4,546,059 - +0.4
4,546,067 - +1.2
4,546,083 + +0.5
4,546,111 + +0.1
4,546,126 + BWI76_RS21750 0.11 -0.4
4,546,137 + BWI76_RS21750 0.11 -0.2
4,546,697 + BWI76_RS21750 0.51 +0.1
4,546,736 + BWI76_RS21750 0.54 -0.1
4,546,744 - BWI76_RS21750 0.55 +0.3
4,546,781 + BWI76_RS21750 0.57 +0.1
4,546,801 - BWI76_RS21750 0.59 +0.0

Or see this region's nucleotide sequence