Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS17000

Experiment: RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.5 x; and 2,2'-Dipyridyl 50 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16995 and BWI76_RS17000 are separated by 2 nucleotidesBWI76_RS17000 and BWI76_RS17005 are separated by 442 nucleotidesBWI76_RS17005 and BWI76_RS17010 are separated by 11 nucleotides BWI76_RS16995: BWI76_RS16995 - Fe-S cluster assembly protein SufB, at 3,524,329 to 3,525,816 _RS16995 BWI76_RS17000: BWI76_RS17000 - Fe-S cluster assembly scaffold SufA, at 3,525,819 to 3,526,196 _RS17000 BWI76_RS17005: BWI76_RS17005 - hypothetical protein, at 3,526,639 to 3,527,151 _RS17005 BWI76_RS17010: BWI76_RS17010 - fimbrial protein, at 3,527,163 to 3,528,110 _RS17010 Position (kb) 3525 3526 3527Strain fitness (log2 ratio) -2 -1 0 1at 3524.899 kb on - strand, within BWI76_RS16995at 3525.230 kb on + strand, within BWI76_RS16995at 3525.336 kb on - strand, within BWI76_RS16995at 3525.491 kb on + strand, within BWI76_RS16995at 3525.532 kb on + strand, within BWI76_RS16995at 3525.545 kb on - strand, within BWI76_RS16995at 3525.897 kb on + strand, within BWI76_RS17000at 3525.998 kb on - strand, within BWI76_RS17000at 3526.143 kb on - strand, within BWI76_RS17000at 3526.205 kb on + strandat 3526.459 kb on + strandat 3526.630 kb on - strandat 3526.671 kb on + strandat 3526.854 kb on + strand, within BWI76_RS17005at 3526.958 kb on + strand, within BWI76_RS17005at 3526.965 kb on + strand, within BWI76_RS17005at 3527.044 kb on + strand, within BWI76_RS17005at 3527.050 kb on + strand, within BWI76_RS17005at 3527.188 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.5 x; and 2,2'-Dipyridyl 50 uM
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3,524,899 - BWI76_RS16995 0.38 -0.2
3,525,230 + BWI76_RS16995 0.61 -2.2
3,525,336 - BWI76_RS16995 0.68 -1.4
3,525,491 + BWI76_RS16995 0.78 -1.8
3,525,532 + BWI76_RS16995 0.81 -0.6
3,525,545 - BWI76_RS16995 0.82 -0.5
3,525,897 + BWI76_RS17000 0.21 -2.4
3,525,998 - BWI76_RS17000 0.47 -1.5
3,526,143 - BWI76_RS17000 0.86 -2.1
3,526,205 + -1.5
3,526,459 + +0.4
3,526,630 - +0.3
3,526,671 + -0.8
3,526,854 + BWI76_RS17005 0.42 -0.1
3,526,958 + BWI76_RS17005 0.62 -0.7
3,526,965 + BWI76_RS17005 0.64 +0.2
3,527,044 + BWI76_RS17005 0.79 +0.3
3,527,050 + BWI76_RS17005 0.80 -0.7
3,527,188 + +0.1

Or see this region's nucleotide sequence