Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS21415 and HSERO_RS21420 overlap by 2 nucleotides HSERO_RS21420 and HSERO_RS21425 are separated by 63 nucleotides HSERO_RS21425 and HSERO_RS21430 overlap by 4 nucleotides
HSERO_RS21415: HSERO_RS21415 - lytic transglycosylase, at 4,902,614 to 4,904,614
_RS21415
HSERO_RS21420: HSERO_RS21420 - 5-formyltetrahydrofolate cyclo-ligase, at 4,904,613 to 4,905,245
_RS21420
HSERO_RS21425: HSERO_RS21425 - 5,10-methylenetetrahydrofolate reductase, at 4,905,309 to 4,906,157
_RS21425
HSERO_RS21430: HSERO_RS21430 - membrane protein, at 4,906,154 to 4,906,507
_RS21430
Position (kb) 4904
4905
4906 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4903.744 kb on - strand at 4903.788 kb on + strand, within HSERO_RS21415 at 4903.872 kb on + strand at 4904.055 kb on + strand, within HSERO_RS21415 at 4904.159 kb on - strand, within HSERO_RS21415 at 4904.262 kb on + strand, within HSERO_RS21415 at 4904.267 kb on + strand, within HSERO_RS21415 at 4904.654 kb on + strand at 4904.709 kb on - strand, within HSERO_RS21420 at 4904.726 kb on + strand, within HSERO_RS21420 at 4904.747 kb on + strand, within HSERO_RS21420 at 4904.747 kb on + strand, within HSERO_RS21420 at 4904.839 kb on + strand, within HSERO_RS21420 at 4905.002 kb on + strand, within HSERO_RS21420 at 4905.018 kb on - strand, within HSERO_RS21420 at 4905.275 kb on - strand at 4905.343 kb on + strand at 4905.436 kb on - strand, within HSERO_RS21425 at 4905.626 kb on - strand, within HSERO_RS21425 at 4905.671 kb on - strand, within HSERO_RS21425 at 4905.863 kb on + strand, within HSERO_RS21425 at 4905.917 kb on + strand, within HSERO_RS21425 at 4905.960 kb on + strand, within HSERO_RS21425 at 4905.989 kb on - strand, within HSERO_RS21425 at 4906.015 kb on + strand, within HSERO_RS21425 at 4906.206 kb on + strand, within HSERO_RS21430 at 4906.209 kb on + strand, within HSERO_RS21430 at 4906.234 kb on - strand, within HSERO_RS21430
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 4,903,744 - +1.3 4,903,788 + HSERO_RS21415 0.59 +0.0 4,903,872 + -0.1 4,904,055 + HSERO_RS21415 0.72 +0.0 4,904,159 - HSERO_RS21415 0.77 -0.1 4,904,262 + HSERO_RS21415 0.82 +0.5 4,904,267 + HSERO_RS21415 0.83 -1.2 4,904,654 + +1.0 4,904,709 - HSERO_RS21420 0.15 +2.5 4,904,726 + HSERO_RS21420 0.18 +0.7 4,904,747 + HSERO_RS21420 0.21 +0.9 4,904,747 + HSERO_RS21420 0.21 +0.8 4,904,839 + HSERO_RS21420 0.36 -0.2 4,905,002 + HSERO_RS21420 0.61 +1.3 4,905,018 - HSERO_RS21420 0.64 +0.5 4,905,275 - -0.3 4,905,343 + -0.1 4,905,436 - HSERO_RS21425 0.15 -1.2 4,905,626 - HSERO_RS21425 0.37 -0.5 4,905,671 - HSERO_RS21425 0.43 -0.2 4,905,863 + HSERO_RS21425 0.65 -2.0 4,905,917 + HSERO_RS21425 0.72 +2.3 4,905,960 + HSERO_RS21425 0.77 -1.3 4,905,989 - HSERO_RS21425 0.80 +1.3 4,906,015 + HSERO_RS21425 0.83 -0.8 4,906,206 + HSERO_RS21430 0.15 +0.6 4,906,209 + HSERO_RS21430 0.16 -0.7 4,906,234 - HSERO_RS21430 0.23 -1.3
Or see this region's nucleotide sequence