Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25335

Experiment: RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5 x; and 2,2'-Dipyridyl 50 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25325 and BWI76_RS25330 are separated by 92 nucleotidesBWI76_RS25330 and BWI76_RS25335 are separated by 3 nucleotidesBWI76_RS25335 and BWI76_RS25340 overlap by 2 nucleotidesBWI76_RS25340 and BWI76_RS25345 are separated by 109 nucleotidesBWI76_RS25345 and BWI76_RS25350 are separated by 15 nucleotides BWI76_RS25325: BWI76_RS25325 - hypothetical protein, at 5,267,036 to 5,267,443 _RS25325 BWI76_RS25330: BWI76_RS25330 - stringent starvation protein B, at 5,267,536 to 5,268,024 _RS25330 BWI76_RS25335: BWI76_RS25335 - stringent starvation protein A, at 5,268,028 to 5,268,666 _RS25335 BWI76_RS25340: BWI76_RS25340 - hypothetical protein, at 5,268,665 to 5,268,868 _RS25340 BWI76_RS25345: BWI76_RS25345 - 30S ribosomal protein S9, at 5,268,978 to 5,269,370 _RS25345 BWI76_RS25350: BWI76_RS25350 - 50S ribosomal protein L13, at 5,269,386 to 5,269,814 _RS25350 Position (kb) 5268 5269Strain fitness (log2 ratio) -2 -1 0 1at 5267.041 kb on - strandat 5268.121 kb on - strand, within BWI76_RS25335at 5268.295 kb on - strand, within BWI76_RS25335at 5268.320 kb on + strand, within BWI76_RS25335

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5 x; and 2,2'-Dipyridyl 50 uM
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5,267,041 - +0.4
5,268,121 - BWI76_RS25335 0.15 -2.1
5,268,295 - BWI76_RS25335 0.42 -0.6
5,268,320 + BWI76_RS25335 0.46 -0.4

Or see this region's nucleotide sequence