Experiment: RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5 x; and 2,2'-Dipyridyl 50 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11270 and BWI76_RS11275 are separated by 86 nucleotides BWI76_RS11275 and BWI76_RS11280 overlap by 4 nucleotides BWI76_RS11280 and BWI76_RS11285 are separated by 84 nucleotides
BWI76_RS11270: BWI76_RS11270 - 50S ribosomal protein L16 arginine hydroxylase, at 2,376,613 to 2,377,734
_RS11270
BWI76_RS11275: BWI76_RS11275 - sensor protein PhoQ, at 2,377,821 to 2,379,287
_RS11275
BWI76_RS11280: BWI76_RS11280 - two-component system response regulator PhoP, at 2,379,284 to 2,379,958
_RS11280
BWI76_RS11285: BWI76_RS11285 - transcriptional regulator, at 2,380,043 to 2,380,489
_RS11285
Position (kb)
2377
2378
2379
2380 Strain fitness (log2 ratio)
-2
-1
0
1 at 2377.628 kb on - strand at 2377.827 kb on - strand at 2377.948 kb on + strand at 2378.339 kb on + strand, within BWI76_RS11275 at 2378.608 kb on + strand, within BWI76_RS11275 at 2378.608 kb on + strand, within BWI76_RS11275 at 2378.759 kb on - strand, within BWI76_RS11275 at 2378.772 kb on - strand, within BWI76_RS11275 at 2378.907 kb on + strand, within BWI76_RS11275 at 2379.027 kb on - strand, within BWI76_RS11275 at 2379.106 kb on + strand, within BWI76_RS11275 at 2379.930 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5 x; and 2,2'-Dipyridyl 50 uM remove 2,377,628 - -0.6 2,377,827 - -0.2 2,377,948 + +0.2 2,378,339 + BWI76_RS11275 0.35 -0.4 2,378,608 + BWI76_RS11275 0.54 -0.7 2,378,608 + BWI76_RS11275 0.54 -1.9 2,378,759 - BWI76_RS11275 0.64 -0.1 2,378,772 - BWI76_RS11275 0.65 -1.3 2,378,907 + BWI76_RS11275 0.74 -1.3 2,379,027 - BWI76_RS11275 0.82 -1.1 2,379,106 + BWI76_RS11275 0.88 -0.1 2,379,930 + -0.7
Or see this region's nucleotide sequence