Experiment: RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.5 x; and Iron (II) 0.209 mg/mL 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt BWI76_RS20435 and BWI76_RS20440 are separated by 37 nucleotides BWI76_RS20440 and BWI76_RS20445 are separated by 167 nucleotides BWI76_RS20445 and BWI76_RS20450 are separated by 20 nucleotides 
        BWI76_RS20435: BWI76_RS20435 - chorismate synthase, at 4,277,326 to 4,278,411 
        _RS20435 
         
        
        BWI76_RS20440: BWI76_RS20440 - 50S ribosomal protein L3 N(5)-glutamine methyltransferase, at 4,278,449 to 4,279,381 
        _RS20440 
         
        
        BWI76_RS20445: BWI76_RS20445 - endonuclease SmrB, at 4,279,549 to 4,280,100 
        _RS20445 
         
        
        BWI76_RS20450: BWI76_RS20450 - phosphohistidine phosphatase, at 4,280,121 to 4,280,606 
        _RS20450 
         Position (kb) 4278 
4279 
4280 Strain fitness (log2 ratio) -4 
-3 
-2 
-1 
0 
1 at 4277.528 kb on + strand, within BWI76_RS20435 at 4278.248 kb on - strand, within BWI76_RS20435 at 4278.348 kb on - strand at 4278.588 kb on - strand, within BWI76_RS20440 at 4278.639 kb on + strand, within BWI76_RS20440 at 4278.867 kb on + strand, within BWI76_RS20440 at 4278.867 kb on + strand, within BWI76_RS20440 at 4279.395 kb on - strand at 4279.396 kb on - strand  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.5 x; and Iron (II) 0.209 mg/mL remove 4,277,528 +  BWI76_RS20435 0.19  -1.8 4,278,248 -  BWI76_RS20435 0.85  -3.3 4,278,348 -  -4.4 4,278,588 -  BWI76_RS20440 0.15  +0.1 4,278,639 +  BWI76_RS20440 0.20  -4.4 4,278,867 +  BWI76_RS20440 0.45  -2.3 4,278,867 +  BWI76_RS20440 0.45  -1.4 4,279,395 -  -1.2 4,279,396 -  -0.9 
Or see this region's nucleotide sequence