Experiment: RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.5 x; and Iron (II) 0.209 mg/mL 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt BWI76_RS18180 and BWI76_RS18185 are separated by 53 nucleotides BWI76_RS18185 and BWI76_RS18190 are separated by 39 nucleotides BWI76_RS18190 and BWI76_RS18195 overlap by 4 nucleotides  
        
        BWI76_RS18180: BWI76_RS18180 - hypothetical protein, at 3,777,514 to 3,778,332 
         
        _RS18180 
         
        
        BWI76_RS18185: BWI76_RS18185 - membrane protein, at 3,778,386 to 3,778,781 
         
        _RS18185 
         
        
        BWI76_RS18190: BWI76_RS18190 - carboxy-S-adenosyl-L-methionine synthase CmoA, at 3,778,821 to 3,779,564 
         
        _RS18190 
         
        
        BWI76_RS18195: BWI76_RS18195 - tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB, at 3,779,561 to 3,780,577 
         
        _RS18195 
         Position (kb)  
3778 
 
3779 
 
3780 Strain fitness (log2 ratio)  
-2 
 
-1 
 
0 
 
1 at 3777.930 kb on + strand, within BWI76_RS18180 at 3777.977 kb on - strand, within BWI76_RS18180 at 3778.454 kb on + strand, within BWI76_RS18185 at 3778.478 kb on - strand, within BWI76_RS18185 at 3778.484 kb on - strand, within BWI76_RS18185 at 3778.603 kb on - strand, within BWI76_RS18185 at 3778.605 kb on + strand, within BWI76_RS18185 at 3778.649 kb on + strand, within BWI76_RS18185 at 3778.744 kb on + strand at 3778.752 kb on - strand at 3779.110 kb on - strand, within BWI76_RS18190 at 3779.150 kb on + strand, within BWI76_RS18190 at 3779.775 kb on + strand, within BWI76_RS18195 at 3779.814 kb on + strand, within BWI76_RS18195 at 3780.107 kb on - strand, within BWI76_RS18195 at 3780.317 kb on - strand, within BWI76_RS18195  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.5 x; and Iron (II) 0.209 mg/mL             remove   3,777,930   +    BWI76_RS18180   0.51  +0.1   3,777,977   -    BWI76_RS18180   0.57  -1.0   3,778,454   +    BWI76_RS18185   0.17  +0.0   3,778,478   -    BWI76_RS18185   0.23  -0.5   3,778,484   -    BWI76_RS18185   0.25  +0.2   3,778,603   -    BWI76_RS18185   0.55  -0.2   3,778,605   +    BWI76_RS18185   0.55  +0.4   3,778,649   +    BWI76_RS18185   0.66  -0.5   3,778,744   +        +0.3   3,778,752   -        -0.3   3,779,110   -    BWI76_RS18190   0.39  -2.0   3,779,150   +    BWI76_RS18190   0.44  -0.4   3,779,775   +    BWI76_RS18195   0.21  -0.1   3,779,814   +    BWI76_RS18195   0.25  -0.2   3,780,107   -    BWI76_RS18195   0.54  -1.1   3,780,317   -    BWI76_RS18195   0.74  -0.2 
 
Or see this region's nucleotide sequence